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Divalent toxoids loaded stable chitosan-glucomannan nanoassemblies for efficient systemic, mucosal and cellular immunostimulatory response following oral administration.
Int J Pharm. 2015 Jun 20; 487(1-2):292-304.IJ

Abstract

The present study reports dual tetanus and diphtheria toxoids loaded stable chitosan-glucomannan nanoassemblies (sCh-GM-NAs) formulated using tandem ionic gelation technique for oral mucosal immunization. The stable, lyophilized sCh-GM-NAs exhibited ~152 nm particle size and ~85% EE of both the toxoids. The lyophilized sCh-GM-NAs displayed excellent stability in biomimetic media and preserved chemical, conformation and biological stability of encapsulated toxoids. The higher intracellular APCs uptake of sCh-GM-NAs was concentration and time dependent which may be attributed to the receptor mediated endocytosis via mannose and glucose receptor. The higher Caco-2 uptake of sCh-GM-NAs was further confirmed by ex vivo intestinal uptake studies. The in vivo evaluation revealed that sCh-GM-NAs posed significantly (p<0.001) higher humoral, mucosal and cellular immune response than other counterparts by eliciting complete protective levels of anti-TT and anti-DT (~0.1 IU/mL) antibodies. Importantly, commercial 'Dual antigen' vaccine administered through oral or intramuscular route was unable to elicit all type of immune response. Conclusively, sCh-GM-NAs could be considered as promising vaccine adjuvant for oral mucosal immunization.

Authors+Show Affiliations

Centre for Pharmaceutical Nanotechnology, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, SAS Nagar, Punjab 160062, India.Centre for Pharmaceutical Nanotechnology, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, SAS Nagar, Punjab 160062, India.Centre for Pharmaceutical Nanotechnology, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, SAS Nagar, Punjab 160062, India.Centre for Pharmaceutical Nanotechnology, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, SAS Nagar, Punjab 160062, India. Electronic address: sanyogjain@niper.ac.in.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25895719

Citation

Harde, Harshad, et al. "Divalent Toxoids Loaded Stable Chitosan-glucomannan Nanoassemblies for Efficient Systemic, Mucosal and Cellular Immunostimulatory Response Following Oral Administration." International Journal of Pharmaceutics, vol. 487, no. 1-2, 2015, pp. 292-304.
Harde H, Siddhapura K, Agrawal AK, et al. Divalent toxoids loaded stable chitosan-glucomannan nanoassemblies for efficient systemic, mucosal and cellular immunostimulatory response following oral administration. Int J Pharm. 2015;487(1-2):292-304.
Harde, H., Siddhapura, K., Agrawal, A. K., & Jain, S. (2015). Divalent toxoids loaded stable chitosan-glucomannan nanoassemblies for efficient systemic, mucosal and cellular immunostimulatory response following oral administration. International Journal of Pharmaceutics, 487(1-2), 292-304. https://doi.org/10.1016/j.ijpharm.2015.04.042
Harde H, et al. Divalent Toxoids Loaded Stable Chitosan-glucomannan Nanoassemblies for Efficient Systemic, Mucosal and Cellular Immunostimulatory Response Following Oral Administration. Int J Pharm. 2015 Jun 20;487(1-2):292-304. PubMed PMID: 25895719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Divalent toxoids loaded stable chitosan-glucomannan nanoassemblies for efficient systemic, mucosal and cellular immunostimulatory response following oral administration. AU - Harde,Harshad, AU - Siddhapura,Krupa, AU - Agrawal,Ashish Kumar, AU - Jain,Sanyog, Y1 - 2015/04/17/ PY - 2014/12/27/received PY - 2015/04/14/revised PY - 2015/04/15/accepted PY - 2015/4/22/entrez PY - 2015/4/22/pubmed PY - 2016/2/18/medline KW - Chitosan-glucomannan nanoassemblies KW - Glucomannosylation KW - Mucosal immunity KW - Stability KW - Vaccine SP - 292 EP - 304 JF - International journal of pharmaceutics JO - Int J Pharm VL - 487 IS - 1-2 N2 - The present study reports dual tetanus and diphtheria toxoids loaded stable chitosan-glucomannan nanoassemblies (sCh-GM-NAs) formulated using tandem ionic gelation technique for oral mucosal immunization. The stable, lyophilized sCh-GM-NAs exhibited ~152 nm particle size and ~85% EE of both the toxoids. The lyophilized sCh-GM-NAs displayed excellent stability in biomimetic media and preserved chemical, conformation and biological stability of encapsulated toxoids. The higher intracellular APCs uptake of sCh-GM-NAs was concentration and time dependent which may be attributed to the receptor mediated endocytosis via mannose and glucose receptor. The higher Caco-2 uptake of sCh-GM-NAs was further confirmed by ex vivo intestinal uptake studies. The in vivo evaluation revealed that sCh-GM-NAs posed significantly (p<0.001) higher humoral, mucosal and cellular immune response than other counterparts by eliciting complete protective levels of anti-TT and anti-DT (~0.1 IU/mL) antibodies. Importantly, commercial 'Dual antigen' vaccine administered through oral or intramuscular route was unable to elicit all type of immune response. Conclusively, sCh-GM-NAs could be considered as promising vaccine adjuvant for oral mucosal immunization. SN - 1873-3476 UR - https://www.unboundmedicine.com/medline/citation/25895719/Divalent_toxoids_loaded_stable_chitosan_glucomannan_nanoassemblies_for_efficient_systemic_mucosal_and_cellular_immunostimulatory_response_following_oral_administration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-5173(15)00350-6 DB - PRIME DP - Unbound Medicine ER -