Tags

Type your tag names separated by a space and hit enter

Diallyl trisufide protects against oxygen glucose deprivation -induced apoptosis by scavenging free radicals via the PI3K/Akt -mediated Nrf2/HO-1 signaling pathway in B35 neural cells.
Brain Res. 2015 Jul 21; 1614:38-50.BR

Abstract

Oxidative stress contributes to development of ischemic brain damage. Many antioxidants have been proven effective in ameliorating cerebral ischemia injury by inhibiting oxidative stress. DATS, an organosulfuric component of garlic oil, exhibits antioxidative effects. In present study, we used OGD model to investigate the neuroprotective effects of DATS and the mechanisms related to these effects. B35 neural cells exposed to OGD caused a decrease in cell viability and increases in the percentage of apoptotic cells and the level of intracellular cleaved caspase-3, all of which were markedly attenuated by DATS. Further, DATS treatment significantly increased Nrf2 expression and nuclear translocation, upregulated downstream gene HO-1 and inhibited intracellular ROS and MDA generation, all of which were markedly attenuated in cells transfected with Nrf2-specific siRNA. In addition, inhibition of PI3K/Akt signaling by PI3K-specific siRNA not only decreased the expression level of Nrf2 and HO-1 proteins, but also diminished the antioxidative and neuroprotective effect of DATS. Taken together, these results indicate that DATS protects B35 neural cells against OGD-induced cell injury by inhibiting ROS production via upregulating the PI3K/Akt-mediated Nrf2 pathway, which further activates HO-1. Based on our results, DATS may be a potential candidate for intervention in hypoxic-ischemic brain injuries such as stroke.

Authors+Show Affiliations

Department of Geriatrics, Chengdu Military General Hospital, Chengdu City, Sichuan Provice, People׳s Republic of China.Department of Geriatrics, Chengdu Military General Hospital, Chengdu City, Sichuan Provice, People׳s Republic of China. Electronic address: gaili9937@163.com.Department of Geriatrics, Chengdu Military General Hospital, Chengdu City, Sichuan Provice, People׳s Republic of China.Department of Geriatrics, Chengdu Military General Hospital, Chengdu City, Sichuan Provice, People׳s Republic of China.Department of Geriatrics, Chengdu Military General Hospital, Chengdu City, Sichuan Provice, People׳s Republic of China.Department of Geriatrics, Chengdu Military General Hospital, Chengdu City, Sichuan Provice, People׳s Republic of China.Department of Geriatrics, Chengdu Military General Hospital, Chengdu City, Sichuan Provice, People׳s Republic of China.Department of Geriatrics, Chengdu Military General Hospital, Chengdu City, Sichuan Provice, People׳s Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25896937

Citation

Xu, Xian Hua, et al. "Diallyl Trisufide Protects Against Oxygen Glucose Deprivation -induced Apoptosis By Scavenging Free Radicals Via the PI3K/Akt -mediated Nrf2/HO-1 Signaling Pathway in B35 Neural Cells." Brain Research, vol. 1614, 2015, pp. 38-50.
Xu XH, Li GL, Wang BA, et al. Diallyl trisufide protects against oxygen glucose deprivation -induced apoptosis by scavenging free radicals via the PI3K/Akt -mediated Nrf2/HO-1 signaling pathway in B35 neural cells. Brain Res. 2015;1614:38-50.
Xu, X. H., Li, G. L., Wang, B. A., Qin, Y., Bai, S. R., Rong, J., Deng, T., & Li, Q. (2015). Diallyl trisufide protects against oxygen glucose deprivation -induced apoptosis by scavenging free radicals via the PI3K/Akt -mediated Nrf2/HO-1 signaling pathway in B35 neural cells. Brain Research, 1614, 38-50. https://doi.org/10.1016/j.brainres.2015.04.014
Xu XH, et al. Diallyl Trisufide Protects Against Oxygen Glucose Deprivation -induced Apoptosis By Scavenging Free Radicals Via the PI3K/Akt -mediated Nrf2/HO-1 Signaling Pathway in B35 Neural Cells. Brain Res. 2015 Jul 21;1614:38-50. PubMed PMID: 25896937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diallyl trisufide protects against oxygen glucose deprivation -induced apoptosis by scavenging free radicals via the PI3K/Akt -mediated Nrf2/HO-1 signaling pathway in B35 neural cells. AU - Xu,Xian Hua, AU - Li,Gai Li, AU - Wang,Bing Ang, AU - Qin,Yang, AU - Bai,Shu Rong, AU - Rong,Jian, AU - Deng,Tao, AU - Li,Qiang, Y1 - 2015/04/17/ PY - 2015/01/19/received PY - 2015/03/17/revised PY - 2015/04/09/accepted PY - 2015/4/22/entrez PY - 2015/4/22/pubmed PY - 2016/2/24/medline KW - Apoptosis KW - Diallyl trisulfide KW - Nrf2/HO-1 KW - Oxygen glucose deprivation KW - PI3K/Akt KW - Reactive oxygen species SP - 38 EP - 50 JF - Brain research JO - Brain Res. VL - 1614 N2 - Oxidative stress contributes to development of ischemic brain damage. Many antioxidants have been proven effective in ameliorating cerebral ischemia injury by inhibiting oxidative stress. DATS, an organosulfuric component of garlic oil, exhibits antioxidative effects. In present study, we used OGD model to investigate the neuroprotective effects of DATS and the mechanisms related to these effects. B35 neural cells exposed to OGD caused a decrease in cell viability and increases in the percentage of apoptotic cells and the level of intracellular cleaved caspase-3, all of which were markedly attenuated by DATS. Further, DATS treatment significantly increased Nrf2 expression and nuclear translocation, upregulated downstream gene HO-1 and inhibited intracellular ROS and MDA generation, all of which were markedly attenuated in cells transfected with Nrf2-specific siRNA. In addition, inhibition of PI3K/Akt signaling by PI3K-specific siRNA not only decreased the expression level of Nrf2 and HO-1 proteins, but also diminished the antioxidative and neuroprotective effect of DATS. Taken together, these results indicate that DATS protects B35 neural cells against OGD-induced cell injury by inhibiting ROS production via upregulating the PI3K/Akt-mediated Nrf2 pathway, which further activates HO-1. Based on our results, DATS may be a potential candidate for intervention in hypoxic-ischemic brain injuries such as stroke. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/25896937/Diallyl_trisufide_protects_against_oxygen_glucose_deprivation__induced_apoptosis_by_scavenging_free_radicals_via_the_PI3K/Akt__mediated_Nrf2/HO_1_signaling_pathway_in_B35_neural_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(15)00303-0 DB - PRIME DP - Unbound Medicine ER -