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Adenovirus-mediated expression of human sodium-iodide symporter gene permits in vivo tracking of adipose tissue-derived stem cells in a canine myocardial infarction model.
Nucl Med Biol 2015; 42(7):621-9NM

Abstract

INTRODUCTION

In vivo tracking of the transplanted stem cells is important in pre-clinical research of stem cell therapy for myocardial infarction. We examined the feasibility of adenovirus-mediated sodium iodide symporter (NIS) gene to cell tracking imaging of transplanted stem cells in a canine infarcted myocardium by clinical single photon emission computed tomography (SPECT).

METHODS

Beagle dogs were injected intramyocardially with NIS-expressing adenovirus-transfected canine stem cells (Ad-hNIS-canine ADSCs) a week after myocardial infarction (MI) development. (99m)Tc-methoxyisobutylisonitrile ((99m)Tc-MIBI) and (99m)Tc-pertechnetate ((99m)TcO4(-)) SPECT imaging were performed for assessment of infarcted myocardium and viable stem cell tracking. Transthoracic echocardiography was performed to monitor any functional cardiac changes.

RESULTS

Left ventricular ejection fraction (LVEF) was decreased after LAD ligation. There was no significant difference in EF between the groups with the stem cell or saline injection. (125)I uptake was higher in Ad-hNIS-canine ADSCs than in non-transfected ADSCs. Cell proliferation and differentiation were not affected by hNIS-carrying adenovirus transfection. (99m)Tc-MIBI myocardial SPECT imaging showed decreased radiotracer uptake in the infarcted apex and mid-anterolateral regions. Ad-hNIS-canine ADSCs were identified as a region of focally increased (99m)TcO4(-) uptake at the lateral wall and around the apex of the left ventricle, peaked at 2 days and was observed until day 9.

CONCLUSIONS

Combination of adenovirus-mediated NIS gene transfection and clinical nuclear imaging modalities enables to trace the fate of transplanted stem cells in infarcted myocardium for translational in vivo cell tracking study for prolonged duration.

Authors+Show Affiliations

Department of Veterinary Radiology and Diagnostic Imaging, College of Veterinary Medicine, Konkuk University, Seoul, Korea. Electronic address: alvetrad09@gmail.com.Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.Biostar Stem Cell Research Institute, K-STEMCELL Co., Ltd, Seoul, Korea.Department of Veterinary Radiology and Diagnostic Imaging, College of Veterinary Medicine, Konkuk University, Seoul, Korea.Department of Veterinary Radiology and Diagnostic Imaging, College of Veterinary Medicine, Konkuk University, Seoul, Korea.Department of Veterinary Radiology and Diagnostic Imaging, College of Veterinary Medicine, Konkuk University, Seoul, Korea.Department of Veterinary Radiology and Diagnostic Imaging, College of Veterinary Medicine, Konkuk University, Seoul, Korea.Department of Veterinary Radiology and Diagnostic Imaging, College of Veterinary Medicine, Konkuk University, Seoul, Korea.Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University, Seoul, Korea.Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University, Seoul, Korea.Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Korea. Electronic address: yjlee@kirams.re.kr.Department of Veterinary Radiology and Diagnostic Imaging, College of Veterinary Medicine, Konkuk University, Seoul, Korea. Electronic address: eomkd@konkuk.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25899941

Citation

Lee, Ah Ra, et al. "Adenovirus-mediated Expression of Human Sodium-iodide Symporter Gene Permits in Vivo Tracking of Adipose Tissue-derived Stem Cells in a Canine Myocardial Infarction Model." Nuclear Medicine and Biology, vol. 42, no. 7, 2015, pp. 621-9.
Lee AR, Woo SK, Kang SK, et al. Adenovirus-mediated expression of human sodium-iodide symporter gene permits in vivo tracking of adipose tissue-derived stem cells in a canine myocardial infarction model. Nucl Med Biol. 2015;42(7):621-9.
Lee, A. R., Woo, S. K., Kang, S. K., Lee, S. Y., Lee, M. Y., Park, N. W., ... Eom, K. D. (2015). Adenovirus-mediated expression of human sodium-iodide symporter gene permits in vivo tracking of adipose tissue-derived stem cells in a canine myocardial infarction model. Nuclear Medicine and Biology, 42(7), pp. 621-9. doi:10.1016/j.nucmedbio.2015.03.006.
Lee AR, et al. Adenovirus-mediated Expression of Human Sodium-iodide Symporter Gene Permits in Vivo Tracking of Adipose Tissue-derived Stem Cells in a Canine Myocardial Infarction Model. Nucl Med Biol. 2015;42(7):621-9. PubMed PMID: 25899941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adenovirus-mediated expression of human sodium-iodide symporter gene permits in vivo tracking of adipose tissue-derived stem cells in a canine myocardial infarction model. AU - Lee,Ah Ra, AU - Woo,Sang Keun, AU - Kang,Sung Keun, AU - Lee,Seung Yeoun, AU - Lee,Mi Young, AU - Park,Noh Won, AU - Song,Sun Hye, AU - Lee,So Yun, AU - Nahm,Sang Soep, AU - Yu,Ji Eun, AU - Kim,Min Hwan, AU - Yoo,Ran Ji, AU - Kang,Joo Hyun, AU - Lee,Yong Jin, AU - Eom,Ki Dong, Y1 - 2015/03/27/ PY - 2014/05/27/received PY - 2015/02/02/revised PY - 2015/03/16/accepted PY - 2015/4/23/entrez PY - 2015/4/23/pubmed PY - 2016/2/18/medline KW - Adenovirus KW - Myocardial infarction KW - NIS gene KW - Nuclear imaging KW - Stem cell tracking SP - 621 EP - 9 JF - Nuclear medicine and biology JO - Nucl. Med. Biol. VL - 42 IS - 7 N2 - INTRODUCTION: In vivo tracking of the transplanted stem cells is important in pre-clinical research of stem cell therapy for myocardial infarction. We examined the feasibility of adenovirus-mediated sodium iodide symporter (NIS) gene to cell tracking imaging of transplanted stem cells in a canine infarcted myocardium by clinical single photon emission computed tomography (SPECT). METHODS: Beagle dogs were injected intramyocardially with NIS-expressing adenovirus-transfected canine stem cells (Ad-hNIS-canine ADSCs) a week after myocardial infarction (MI) development. (99m)Tc-methoxyisobutylisonitrile ((99m)Tc-MIBI) and (99m)Tc-pertechnetate ((99m)TcO4(-)) SPECT imaging were performed for assessment of infarcted myocardium and viable stem cell tracking. Transthoracic echocardiography was performed to monitor any functional cardiac changes. RESULTS: Left ventricular ejection fraction (LVEF) was decreased after LAD ligation. There was no significant difference in EF between the groups with the stem cell or saline injection. (125)I uptake was higher in Ad-hNIS-canine ADSCs than in non-transfected ADSCs. Cell proliferation and differentiation were not affected by hNIS-carrying adenovirus transfection. (99m)Tc-MIBI myocardial SPECT imaging showed decreased radiotracer uptake in the infarcted apex and mid-anterolateral regions. Ad-hNIS-canine ADSCs were identified as a region of focally increased (99m)TcO4(-) uptake at the lateral wall and around the apex of the left ventricle, peaked at 2 days and was observed until day 9. CONCLUSIONS: Combination of adenovirus-mediated NIS gene transfection and clinical nuclear imaging modalities enables to trace the fate of transplanted stem cells in infarcted myocardium for translational in vivo cell tracking study for prolonged duration. SN - 1872-9614 UR - https://www.unboundmedicine.com/medline/citation/25899941/Adenovirus_mediated_expression_of_human_sodium_iodide_symporter_gene_permits_in_vivo_tracking_of_adipose_tissue_derived_stem_cells_in_a_canine_myocardial_infarction_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969-8051(15)00058-X DB - PRIME DP - Unbound Medicine ER -