Tags

Type your tag names separated by a space and hit enter

Increased expression of six ZIP family genes by zinc (Zn) deficiency is associated with enhanced uptake and root-to-shoot translocation of Zn in barley (Hordeum vulgare).
New Phytol. 2015 Sep; 207(4):1097-109.NP

Abstract

Low zinc (Zn) in soils reduces yield and grain Zn content. Regulation of ZRT/IRT-like protein (ZIP) family genes is a major mechanism in plant adaptation to low and fluctuating Zn in soil. Although several Zn deficiency-inducible ZIP genes are identified in cereals, there has been no systematic study on the association of Zn deficiency-induced uptake and root-to-shoot translocation with expression of ZIP family genes. We measured Zn deficiency-induced uptake and root-to-shoot translocation of Zn in barley (Hordeum vulgare) plants by resupplying 0.5 μM Zn, and quantified the transcripts of thirteen HvZIP genes. Subcellular localization and tissue-specific expression were also determined for Zn deficiency-inducible HvZIP genes. Zn deficiency enhanced the capacity of uptake and root-to-shoot translocation of Zn, and sustained the enhanced capacity for 6 d after Zn resupply. Six HvZIP genes were highly induced in roots of Zn-deficient plants, and their proteins were localized in the plasma membrane. Tissue-specific expression in roots supports their roles in uptake and root-to-shoot translocation of Zn under low Zn conditions. Our results provide a comprehensive view on the physiological roles of ZIP genes in plant adaptation to low and fluctuating Zn in soil, and pave the way for development of new strategies to improve Zn-deficiency tolerance and biofortification in cereals.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Tiong J
Australian Centre for Plant Functional Genomics, School of Agriculture Food and Wine, the University of Adelaide, PMB1, Glen Osmond, SA, 5064, Australia.
McDonald G
School of Agriculture, Food and Wine, the University of Adelaide, Glen Osmond, SA, 5064, Australia.
Genc Y
School of Agriculture, Food and Wine, the University of Adelaide, Glen Osmond, SA, 5064, Australia.
Shirley N
ARC Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, the University of Adelaide, Glen Osmond, SA, 5064, Australia.
Langridge P
Australian Centre for Plant Functional Genomics, School of Agriculture Food and Wine, the University of Adelaide, PMB1, Glen Osmond, SA, 5064, Australia.
Huang CY
Australian Centre for Plant Functional Genomics, School of Agriculture Food and Wine, the University of Adelaide, PMB1, Glen Osmond, SA, 5064, Australia.

MeSH

Biological TransportGene Expression Regulation, PlantGenes, PlantHordeumModels, BiologicalMultigene FamilyPhylogenyPlant EpidermisPlant ProteinsPlant RootsPlant ShootsRNA, MessengerRecombinant Fusion ProteinsSoilSpecies SpecificitySubcellular FractionsZinc

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25904503

Citation

Tiong, Jingwen, et al. "Increased Expression of Six ZIP Family Genes By Zinc (Zn) Deficiency Is Associated With Enhanced Uptake and Root-to-shoot Translocation of Zn in Barley (Hordeum Vulgare)." The New Phytologist, vol. 207, no. 4, 2015, pp. 1097-109.
Tiong J, McDonald G, Genc Y, et al. Increased expression of six ZIP family genes by zinc (Zn) deficiency is associated with enhanced uptake and root-to-shoot translocation of Zn in barley (Hordeum vulgare). New Phytol. 2015;207(4):1097-109.
Tiong, J., McDonald, G., Genc, Y., Shirley, N., Langridge, P., & Huang, C. Y. (2015). Increased expression of six ZIP family genes by zinc (Zn) deficiency is associated with enhanced uptake and root-to-shoot translocation of Zn in barley (Hordeum vulgare). The New Phytologist, 207(4), 1097-109. https://doi.org/10.1111/nph.13413
Tiong J, et al. Increased Expression of Six ZIP Family Genes By Zinc (Zn) Deficiency Is Associated With Enhanced Uptake and Root-to-shoot Translocation of Zn in Barley (Hordeum Vulgare). New Phytol. 2015;207(4):1097-109. PubMed PMID: 25904503.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased expression of six ZIP family genes by zinc (Zn) deficiency is associated with enhanced uptake and root-to-shoot translocation of Zn in barley (Hordeum vulgare). AU - Tiong,Jingwen, AU - McDonald,Glenn, AU - Genc,Yusuf, AU - Shirley,Neil, AU - Langridge,Peter, AU - Huang,Chun Y, Y1 - 2015/04/22/ PY - 2015/02/20/received PY - 2015/03/17/accepted PY - 2015/4/24/entrez PY - 2015/4/24/pubmed PY - 2016/5/5/medline KW - ZRT/IRT-like protein (ZIP) KW - Zn resupply KW - Zn uptake KW - barley (Hordeum vulgare) KW - root KW - translocation KW - zinc (Zn) deficiency SP - 1097 EP - 109 JF - The New phytologist JO - New Phytol VL - 207 IS - 4 N2 - Low zinc (Zn) in soils reduces yield and grain Zn content. Regulation of ZRT/IRT-like protein (ZIP) family genes is a major mechanism in plant adaptation to low and fluctuating Zn in soil. Although several Zn deficiency-inducible ZIP genes are identified in cereals, there has been no systematic study on the association of Zn deficiency-induced uptake and root-to-shoot translocation with expression of ZIP family genes. We measured Zn deficiency-induced uptake and root-to-shoot translocation of Zn in barley (Hordeum vulgare) plants by resupplying 0.5 μM Zn, and quantified the transcripts of thirteen HvZIP genes. Subcellular localization and tissue-specific expression were also determined for Zn deficiency-inducible HvZIP genes. Zn deficiency enhanced the capacity of uptake and root-to-shoot translocation of Zn, and sustained the enhanced capacity for 6 d after Zn resupply. Six HvZIP genes were highly induced in roots of Zn-deficient plants, and their proteins were localized in the plasma membrane. Tissue-specific expression in roots supports their roles in uptake and root-to-shoot translocation of Zn under low Zn conditions. Our results provide a comprehensive view on the physiological roles of ZIP genes in plant adaptation to low and fluctuating Zn in soil, and pave the way for development of new strategies to improve Zn-deficiency tolerance and biofortification in cereals. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/25904503/Increased_expression_of_six_ZIP_family_genes_by_zinc__Zn__deficiency_is_associated_with_enhanced_uptake_and_root_to_shoot_translocation_of_Zn_in_barley__Hordeum_vulgare__ DB - PRIME DP - Unbound Medicine ER -