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[Phenotypic methods for detection of methicillin-resistant Staphylococcus aureus].
Rev Esp Quimioter. 2015 Apr; 28(2):98-100.RE

Abstract

BACKGROUND

Cefoxitin is a potent inducer of the mecA gene. It is currently as a screening recommended method for presumptive identification of isolates of methicillin resistant Staphylococcus aureus (MRSA). The aim of the study was to compare the sensitivity and specificity of the cefoxitin disc diffusion (30 μg) to oxacillin agar screening from detection of the mecA gene by PCR.

METHODS

Three hundred thirty-one strains of S. aureus isolated from blood cultures of patients from hospitals in Lima were used in the study. The following tests were performed: oxacillin screening agar (plates were inoculated with 4% NaCl and 6 mg/L of oxacillin), cefoxitin disc diffusion test (30 ug) and PCR to amplify the mecA gene.

RESULTS

The mecA gene was detected in 165 out of 331 S. aureus isolates. Thus, the frequency of detection of MRSA was 50%. The evaluation of the cefoxitin disc diffusion test showed a 96.3% and 90.9% of sensitivity and specificity, respectively.

CONCLUSION

Cefoxitin disc diffusion test correlated well with detection of the mecA gene by PCR. Therefore, this test can be an alternative to PCR for detection of MRSA in limited resources settings.

Links

Publisher Full Text

Authors+Show Affiliations

Horna G
Gertrudis Horna, Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano He-redia, Lima Perú. Av.Honorio Delgado # 430 - Urb Ingenieria - SMP - Lima- Perú. sara.horna@upch.pe.
Astocondor L
No affiliation info available
Jacobs J
No affiliation info available
García C
No affiliation info available

MeSH

AdenosineAnti-Bacterial AgentsCefoxitinFalse Positive ReactionsHumansMethicillin ResistanceMethicillin-Resistant Staphylococcus aureusMicrobial Sensitivity TestsOxacillinPhenotypePolymerase Chain ReactionStaphylococcal Infections

Pub Type(s)

English Abstract
Journal Article

Language

spa

PubMed ID

25904517

Citation

Horna, G, et al. "[Phenotypic Methods for Detection of Methicillin-resistant Staphylococcus Aureus]." Revista Espanola De Quimioterapia : Publicacion Oficial De La Sociedad Espanola De Quimioterapia, vol. 28, no. 2, 2015, pp. 98-100.
Horna G, Astocondor L, Jacobs J, et al. [Phenotypic methods for detection of methicillin-resistant Staphylococcus aureus]. Rev Esp Quimioter. 2015;28(2):98-100.
Horna, G., Astocondor, L., Jacobs, J., & García, C. (2015). [Phenotypic methods for detection of methicillin-resistant Staphylococcus aureus]. Revista Espanola De Quimioterapia : Publicacion Oficial De La Sociedad Espanola De Quimioterapia, 28(2), 98-100.
Horna G, et al. [Phenotypic Methods for Detection of Methicillin-resistant Staphylococcus Aureus]. Rev Esp Quimioter. 2015;28(2):98-100. PubMed PMID: 25904517.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Phenotypic methods for detection of methicillin-resistant Staphylococcus aureus]. AU - Horna,G, AU - Astocondor,L, AU - Jacobs,J, AU - García,C, PY - 2015/4/24/entrez PY - 2015/4/24/pubmed PY - 2016/1/20/medline SP - 98 EP - 100 JF - Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia JO - Rev Esp Quimioter VL - 28 IS - 2 N2 - BACKGROUND: Cefoxitin is a potent inducer of the mecA gene. It is currently as a screening recommended method for presumptive identification of isolates of methicillin resistant Staphylococcus aureus (MRSA). The aim of the study was to compare the sensitivity and specificity of the cefoxitin disc diffusion (30 μg) to oxacillin agar screening from detection of the mecA gene by PCR. METHODS: Three hundred thirty-one strains of S. aureus isolated from blood cultures of patients from hospitals in Lima were used in the study. The following tests were performed: oxacillin screening agar (plates were inoculated with 4% NaCl and 6 mg/L of oxacillin), cefoxitin disc diffusion test (30 ug) and PCR to amplify the mecA gene. RESULTS: The mecA gene was detected in 165 out of 331 S. aureus isolates. Thus, the frequency of detection of MRSA was 50%. The evaluation of the cefoxitin disc diffusion test showed a 96.3% and 90.9% of sensitivity and specificity, respectively. CONCLUSION: Cefoxitin disc diffusion test correlated well with detection of the mecA gene by PCR. Therefore, this test can be an alternative to PCR for detection of MRSA in limited resources settings. SN - 1988-9518 UR - https://www.unboundmedicine.com/medline/citation/25904517/[Phenotypic_methods_for_detection_of_methicillin_resistant_Staphylococcus_aureus]_ L2 - http://seq.es/seq/0214-3429/28/2/horna.pdf DB - PRIME DP - Unbound Medicine ER -