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Transcriptome profiling reveals the regulatory mechanism underlying pollination dependent and parthenocarpic fruit set mainly mediated by auxin and gibberellin.
PLoS One. 2015; 10(4):e0125355.Plos

Abstract

BACKGROUND

Fruit set is a key process for crop production in tomato which occurs after successful pollination and fertilization naturally. However, parthenocarpic fruit development can be uncoupled from fertilization triggered by exogenous auxin or gibberellins (GAs). Global transcriptome knowledge during fruit initiation would help to characterize the molecular mechanisms by which these two hormones regulate pollination-dependent and -independent fruit set.

PRINCIPAL FINDINGS

In this work, digital gene expression tag profiling (DGE) technology was applied to compare the transcriptomes from pollinated and 2, 4-D/GA3-treated ovaries. Activation of carbohydrate metabolism, cell division and expansion as well as the down-regulation of MADS-box is a comprehensive regulatory pathway during pollination-dependent and parthenocarpic fruit set. The signaling cascades of auxin and GA are significantly modulated. The feedback regulations of Aux/IAAs and DELLA genes which functioned to fine-tune auxin and GA response respectively play fundamental roles in triggering fruit initiation. In addition, auxin regulates GA synthesis via up-regulation of GA20ox1 and down-regulation of KNOX. Accordingly, the effect of auxin on fruit set is mediated by GA via ARF2 and IAA9 down-regulation, suggesting that both pollination-dependent and parthenocarpic fruit set depend on the crosstalk between auxin and GA.

SIGNIFICANCE

This study characterizes the transcriptomic features of ovary development and more importantly unravels the integral roles of auxin and GA on pollination-dependent and parthenocarpic fruit set.

Authors+Show Affiliations

Genetic Engineering Research Center, School of Life Sciences, Key Laboratory of Functional Gene and New Regulation Technologies Under Chongqing Municipal Education Commission, Chongqing University, Chongqing 400030, China.Genetic Engineering Research Center, School of Life Sciences, Key Laboratory of Functional Gene and New Regulation Technologies Under Chongqing Municipal Education Commission, Chongqing University, Chongqing 400030, China.Genetic Engineering Research Center, School of Life Sciences, Key Laboratory of Functional Gene and New Regulation Technologies Under Chongqing Municipal Education Commission, Chongqing University, Chongqing 400030, China.Genetic Engineering Research Center, School of Life Sciences, Key Laboratory of Functional Gene and New Regulation Technologies Under Chongqing Municipal Education Commission, Chongqing University, Chongqing 400030, China.Genetic Engineering Research Center, School of Life Sciences, Key Laboratory of Functional Gene and New Regulation Technologies Under Chongqing Municipal Education Commission, Chongqing University, Chongqing 400030, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25909657

Citation

Tang, Ning, et al. "Transcriptome Profiling Reveals the Regulatory Mechanism Underlying Pollination Dependent and Parthenocarpic Fruit Set Mainly Mediated By Auxin and Gibberellin." PloS One, vol. 10, no. 4, 2015, pp. e0125355.
Tang N, Deng W, Hu G, et al. Transcriptome profiling reveals the regulatory mechanism underlying pollination dependent and parthenocarpic fruit set mainly mediated by auxin and gibberellin. PLoS One. 2015;10(4):e0125355.
Tang, N., Deng, W., Hu, G., Hu, N., & Li, Z. (2015). Transcriptome profiling reveals the regulatory mechanism underlying pollination dependent and parthenocarpic fruit set mainly mediated by auxin and gibberellin. PloS One, 10(4), e0125355. https://doi.org/10.1371/journal.pone.0125355
Tang N, et al. Transcriptome Profiling Reveals the Regulatory Mechanism Underlying Pollination Dependent and Parthenocarpic Fruit Set Mainly Mediated By Auxin and Gibberellin. PLoS One. 2015;10(4):e0125355. PubMed PMID: 25909657.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome profiling reveals the regulatory mechanism underlying pollination dependent and parthenocarpic fruit set mainly mediated by auxin and gibberellin. AU - Tang,Ning, AU - Deng,Wei, AU - Hu,Guojian, AU - Hu,Nan, AU - Li,Zhengguo, Y1 - 2015/04/24/ PY - 2014/12/05/received PY - 2015/03/11/accepted PY - 2015/4/25/entrez PY - 2015/4/25/pubmed PY - 2016/4/12/medline SP - e0125355 EP - e0125355 JF - PloS one JO - PLoS One VL - 10 IS - 4 N2 - BACKGROUND: Fruit set is a key process for crop production in tomato which occurs after successful pollination and fertilization naturally. However, parthenocarpic fruit development can be uncoupled from fertilization triggered by exogenous auxin or gibberellins (GAs). Global transcriptome knowledge during fruit initiation would help to characterize the molecular mechanisms by which these two hormones regulate pollination-dependent and -independent fruit set. PRINCIPAL FINDINGS: In this work, digital gene expression tag profiling (DGE) technology was applied to compare the transcriptomes from pollinated and 2, 4-D/GA3-treated ovaries. Activation of carbohydrate metabolism, cell division and expansion as well as the down-regulation of MADS-box is a comprehensive regulatory pathway during pollination-dependent and parthenocarpic fruit set. The signaling cascades of auxin and GA are significantly modulated. The feedback regulations of Aux/IAAs and DELLA genes which functioned to fine-tune auxin and GA response respectively play fundamental roles in triggering fruit initiation. In addition, auxin regulates GA synthesis via up-regulation of GA20ox1 and down-regulation of KNOX. Accordingly, the effect of auxin on fruit set is mediated by GA via ARF2 and IAA9 down-regulation, suggesting that both pollination-dependent and parthenocarpic fruit set depend on the crosstalk between auxin and GA. SIGNIFICANCE: This study characterizes the transcriptomic features of ovary development and more importantly unravels the integral roles of auxin and GA on pollination-dependent and parthenocarpic fruit set. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25909657/Transcriptome_profiling_reveals_the_regulatory_mechanism_underlying_pollination_dependent_and_parthenocarpic_fruit_set_mainly_mediated_by_auxin_and_gibberellin_ L2 - https://dx.plos.org/10.1371/journal.pone.0125355 DB - PRIME DP - Unbound Medicine ER -