Tags

Type your tag names separated by a space and hit enter

Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β-dependent epithelial to mesenchymal transition.
J Surg Res. 2015 Jul; 197(1):167-75.JS

Abstract

BACKGROUND

Epithelial to mesenchymal transition (EMT) of alveolar epithelial cells occurs in lung fibrotic diseases. Tanshinone IIA (Tan IIA) has been reported to exert anti-inflammatory effects in pulmonary fibrosis. Nonetheless, whether Tan IIA affects lung fibrosis-related EMT remains unknown and requires for further investigations.

MATERIALS AND METHODS

A single intratracheal instillation of saline containing bleomycin (BLM; 5 mg/kg body weight) was performed to induce pulmonary fibrosis in Sprague-Dawley rats. Rats receiving an instillation of equivoluminal normal saline served as controls. Then, these rats were given a daily intraperitoneal administration of Tan IIA (15 mg/kg body weight) for 28 d before sacrifice. In vitro, recombinant transforming growth factor-beta 1 (TGF-β1; 10 ng/mL) was used to treat human alveolar epithelial A549 cells for 48 h. Tan IIA (10 μM) or control DMSO was used to pretreat cells for 2 h before TGF-β1 stimulation. Rat lung tissue samples and A549 cells were then subjected to further assessments.

RESULTS

Tan IIA was noted to alleviate BLM-induced pulmonary collagen deposition and macrophage infiltration in rats. Epithelial-cadherin expression was decreased after BLM stimulation, whereas α-smooth muscle actin, fibronectin, and vimentin were increased. These expression alterations were partially reversed by Tan IIA. Moreover, Tan IIA suppressed BLM-induced increases in TGF-β1, phosphorylated Smad-2, and -3 in rats. Additionally, pretreatment of Tan IIA inhibited TGF-β1-triggered EMT, reduced collagen Ⅰ production, and blocked TGF-β signal transduction in A549 cells.

CONCLUSIONS

Our research suggests that Tan IIA mitigates BLM-induced pulmonary fibrosis and suppresses TGF-β-dependent EMT of lung alveolar epithelial cells.

Authors+Show Affiliations

Department of Respiratory Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, People's Republic of China.Department of Respiratory Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, People's Republic of China.Department of Pediatrics, The First Affiliated Hospital of Dalian Medical University, Dalian, People's Republic of China.Department of Respiratory Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, People's Republic of China.Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, People's Republic of China.Department of Respiratory Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, People's Republic of China.Department of Nephrology, The First Affiliated Hospital of Dalian Medical University, Dalian, People's Republic of China. Electronic address: linhongli@vip.163.com.Department of Respiratory Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, People's Republic of China. Electronic address: wutaihua@sina.com.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25911951

Citation

Tang, Haiying, et al. "Tanshinone IIA Ameliorates Bleomycin-induced Pulmonary Fibrosis and Inhibits Transforming Growth Factor-beta-β-dependent Epithelial to Mesenchymal Transition." The Journal of Surgical Research, vol. 197, no. 1, 2015, pp. 167-75.
Tang H, He H, Ji H, et al. Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β-dependent epithelial to mesenchymal transition. J Surg Res. 2015;197(1):167-75.
Tang, H., He, H., Ji, H., Gao, L., Mao, J., Liu, J., Lin, H., & Wu, T. (2015). Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β-dependent epithelial to mesenchymal transition. The Journal of Surgical Research, 197(1), 167-75. https://doi.org/10.1016/j.jss.2015.02.062
Tang H, et al. Tanshinone IIA Ameliorates Bleomycin-induced Pulmonary Fibrosis and Inhibits Transforming Growth Factor-beta-β-dependent Epithelial to Mesenchymal Transition. J Surg Res. 2015;197(1):167-75. PubMed PMID: 25911951.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β-dependent epithelial to mesenchymal transition. AU - Tang,Haiying, AU - He,Huanyu, AU - Ji,Hong, AU - Gao,Lili, AU - Mao,Jingwei, AU - Liu,Jia, AU - Lin,Hongli, AU - Wu,Taihua, Y1 - 2015/03/13/ PY - 2014/12/09/received PY - 2015/02/15/revised PY - 2015/02/26/accepted PY - 2015/4/28/entrez PY - 2015/4/29/pubmed PY - 2015/8/19/medline KW - Epithelial to mesenchymal transition KW - Pulmonary fibrosis KW - TGF-β signaling pathway KW - Tanshinone ⅡA SP - 167 EP - 75 JF - The Journal of surgical research JO - J Surg Res VL - 197 IS - 1 N2 - BACKGROUND: Epithelial to mesenchymal transition (EMT) of alveolar epithelial cells occurs in lung fibrotic diseases. Tanshinone IIA (Tan IIA) has been reported to exert anti-inflammatory effects in pulmonary fibrosis. Nonetheless, whether Tan IIA affects lung fibrosis-related EMT remains unknown and requires for further investigations. MATERIALS AND METHODS: A single intratracheal instillation of saline containing bleomycin (BLM; 5 mg/kg body weight) was performed to induce pulmonary fibrosis in Sprague-Dawley rats. Rats receiving an instillation of equivoluminal normal saline served as controls. Then, these rats were given a daily intraperitoneal administration of Tan IIA (15 mg/kg body weight) for 28 d before sacrifice. In vitro, recombinant transforming growth factor-beta 1 (TGF-β1; 10 ng/mL) was used to treat human alveolar epithelial A549 cells for 48 h. Tan IIA (10 μM) or control DMSO was used to pretreat cells for 2 h before TGF-β1 stimulation. Rat lung tissue samples and A549 cells were then subjected to further assessments. RESULTS: Tan IIA was noted to alleviate BLM-induced pulmonary collagen deposition and macrophage infiltration in rats. Epithelial-cadherin expression was decreased after BLM stimulation, whereas α-smooth muscle actin, fibronectin, and vimentin were increased. These expression alterations were partially reversed by Tan IIA. Moreover, Tan IIA suppressed BLM-induced increases in TGF-β1, phosphorylated Smad-2, and -3 in rats. Additionally, pretreatment of Tan IIA inhibited TGF-β1-triggered EMT, reduced collagen Ⅰ production, and blocked TGF-β signal transduction in A549 cells. CONCLUSIONS: Our research suggests that Tan IIA mitigates BLM-induced pulmonary fibrosis and suppresses TGF-β-dependent EMT of lung alveolar epithelial cells. SN - 1095-8673 UR - https://www.unboundmedicine.com/medline/citation/25911951/Tanshinone_IIA_ameliorates_bleomycin_induced_pulmonary_fibrosis_and_inhibits_transforming_growth_factor_beta_β_dependent_epithelial_to_mesenchymal_transition_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(15)00174-2 DB - PRIME DP - Unbound Medicine ER -