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High prevalence of methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene in a semi-extensive red deer (Cervus elaphus hispanicus) farm in Southern Spain.
Vet Microbiol. 2015 Jun 12; 177(3-4):326-31.VM

Abstract

The objective was to determine the prevalence of Staphylococcus aureus nasal carriage in red deer of a semi-extensive farm and in humans in contact with the estate animals, and to characterize obtained isolates. Nasal swabs of 65 deer and 15 humans were seeded on mannitol-salt-agar and oxacillin-resistance-screening-agar-base. Isolates were identified by microbiological and molecular methods. Antimicrobial susceptibility profile was determined for 16 antibiotics by disk-diffusion and the presence of eight antibiotic resistance genes, seven virulence genes and genes of immune-evasion-cluster (IEC) was analyzed by PCR. S. aureus was typed by PFGE-SmaI, spa, agr, SCCmec and MLST. Isolates were detected in 16 deer (24.6%). Eleven S. aureus isolates were methicillin-resistant (MRSA), and five were methicillin-susceptible (MSSA). All MRSA harbored mecC gene and were agr-III/SCCmecXI/ST1945 (four spa-t843 and seven spa-t1535). All mecC-MRSA carried blaZ-SCCmecXI and etd2, were IEC-type-E, and belonged to the same PFGE pattern. The five MSSA were typed as spa-t2420/agr-I/ST133. Regarding humans, S. aureus was recovered from six samples (40%). The isolates were MSSA and were typed as spa-t002/agr-II, spa-t012/agr-III or spa-t822/agr-III and showed different IEC types (A, B, D and F). blaZ and erm(A) genes were detected, as well as cna and tst genes. As conclusion, red deer analyzed in this study are frequent carriers of mecC-MRSA CC130 (16.9%), they are characterized by few resistance and virulence determinants, and by the presence of IEC type-E. Deer could be a source of mecC-MRSA which could potentially be transmitted to other animals, or even to humans.

Authors+Show Affiliations

Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.SaBio, Spanish Wildlife Research Institute IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.SaBio, Spanish Wildlife Research Institute IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain. Electronic address: carmen.torres@unirioja.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25912021

Citation

Gómez, Paula, et al. "High Prevalence of Methicillin-resistant Staphylococcus Aureus (MRSA) Carrying the mecC Gene in a Semi-extensive Red Deer (Cervus Elaphus Hispanicus) Farm in Southern Spain." Veterinary Microbiology, vol. 177, no. 3-4, 2015, pp. 326-31.
Gómez P, Lozano C, González-Barrio D, et al. High prevalence of methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene in a semi-extensive red deer (Cervus elaphus hispanicus) farm in Southern Spain. Vet Microbiol. 2015;177(3-4):326-31.
Gómez, P., Lozano, C., González-Barrio, D., Zarazaga, M., Ruiz-Fons, F., & Torres, C. (2015). High prevalence of methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene in a semi-extensive red deer (Cervus elaphus hispanicus) farm in Southern Spain. Veterinary Microbiology, 177(3-4), 326-31. https://doi.org/10.1016/j.vetmic.2015.03.029
Gómez P, et al. High Prevalence of Methicillin-resistant Staphylococcus Aureus (MRSA) Carrying the mecC Gene in a Semi-extensive Red Deer (Cervus Elaphus Hispanicus) Farm in Southern Spain. Vet Microbiol. 2015 Jun 12;177(3-4):326-31. PubMed PMID: 25912021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High prevalence of methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene in a semi-extensive red deer (Cervus elaphus hispanicus) farm in Southern Spain. AU - Gómez,Paula, AU - Lozano,Carmen, AU - González-Barrio,David, AU - Zarazaga,Myriam, AU - Ruiz-Fons,Francisco, AU - Torres,Carmen, Y1 - 2015/04/14/ PY - 2015/01/31/received PY - 2015/03/19/revised PY - 2015/03/31/accepted PY - 2015/4/28/entrez PY - 2015/4/29/pubmed PY - 2016/1/29/medline KW - CC130 KW - IEC KW - MRSA KW - Red deer KW - S. aureus KW - mecC SP - 326 EP - 31 JF - Veterinary microbiology JO - Vet Microbiol VL - 177 IS - 3-4 N2 - The objective was to determine the prevalence of Staphylococcus aureus nasal carriage in red deer of a semi-extensive farm and in humans in contact with the estate animals, and to characterize obtained isolates. Nasal swabs of 65 deer and 15 humans were seeded on mannitol-salt-agar and oxacillin-resistance-screening-agar-base. Isolates were identified by microbiological and molecular methods. Antimicrobial susceptibility profile was determined for 16 antibiotics by disk-diffusion and the presence of eight antibiotic resistance genes, seven virulence genes and genes of immune-evasion-cluster (IEC) was analyzed by PCR. S. aureus was typed by PFGE-SmaI, spa, agr, SCCmec and MLST. Isolates were detected in 16 deer (24.6%). Eleven S. aureus isolates were methicillin-resistant (MRSA), and five were methicillin-susceptible (MSSA). All MRSA harbored mecC gene and were agr-III/SCCmecXI/ST1945 (four spa-t843 and seven spa-t1535). All mecC-MRSA carried blaZ-SCCmecXI and etd2, were IEC-type-E, and belonged to the same PFGE pattern. The five MSSA were typed as spa-t2420/agr-I/ST133. Regarding humans, S. aureus was recovered from six samples (40%). The isolates were MSSA and were typed as spa-t002/agr-II, spa-t012/agr-III or spa-t822/agr-III and showed different IEC types (A, B, D and F). blaZ and erm(A) genes were detected, as well as cna and tst genes. As conclusion, red deer analyzed in this study are frequent carriers of mecC-MRSA CC130 (16.9%), they are characterized by few resistance and virulence determinants, and by the presence of IEC type-E. Deer could be a source of mecC-MRSA which could potentially be transmitted to other animals, or even to humans. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/25912021/High_prevalence_of_methicillin_resistant_Staphylococcus_aureus__MRSA__carrying_the_mecC_gene_in_a_semi_extensive_red_deer__Cervus_elaphus_hispanicus__farm_in_Southern_Spain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(15)00144-3 DB - PRIME DP - Unbound Medicine ER -