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Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations.
Biotechnol Prog 2015 Jul-Aug; 31(4):937-45BP

Abstract

Bromelain is a set of proteolytic enzymes found in pineapple (Ananas comosus) tissues such as stem, fruit and leaves. Because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. The present study focused on the recovery of bromelain from pineapple peel by liquid-liquid extraction in aqueous two-phase micellar systems (ATPMS), using Triton X-114 (TX-114) and McIlvaine buffer, in the absence and presence of electrolytes CaCl2 and KI; the cloud points of the generated extraction systems were studied by plotting binodal curves. Based on the cloud points, three temperatures were selected for extraction: 30, 33, and 36°C for systems in the absence of salts; 40, 43, and 46°C in the presence of KI; 24, 27, and 30°C in the presence of CaCl2 . Total protein and enzymatic activities were analyzed to monitor bromelain. Employing the ATPMS chosen for extraction (0.5 M KI with 3% TX-114, at pH 6.0, at 40°C), the bromelain extract stability was assessed after incorporation into three cosmetic bases: an anhydrous gel, a cream, and a cream-gel formulation. The cream-gel formulation presented as the most appropriate base to convey bromelain, and its optimal storage conditions were found to be 4.0 ± 0.5°C. The selected ATPMS enabled the extraction of a biomolecule with high added value from waste lined-up in a cosmetic formulation, allowing for exploration of further cosmetic potential.

Authors+Show Affiliations

Dept. of Clinical Pathology, Faculty of Medical Sciences, University of Campinas (Unicamp), Campinas, São Paulo, Brazil. Faculty of Pharmaceutical Sciences, University of Campinas (Unicamp), Campinas, São Paulo, Brazil.Dept. of Clinical Pathology, Faculty of Medical Sciences, University of Campinas (Unicamp), Campinas, São Paulo, Brazil. Faculty of Pharmaceutical Sciences, University of Campinas (Unicamp), Campinas, São Paulo, Brazil.Dept. of Clinical Pathology, Faculty of Medical Sciences, University of Campinas (Unicamp), Campinas, São Paulo, Brazil. Faculty of Pharmaceutical Sciences, University of Campinas (Unicamp), Campinas, São Paulo, Brazil.Dept. of Clinical Pathology, Faculty of Medical Sciences, University of Campinas (Unicamp), Campinas, São Paulo, Brazil. Faculty of Pharmaceutical Sciences, University of Campinas (Unicamp), Campinas, São Paulo, Brazil.Dept. of Clinical Pathology, Faculty of Medical Sciences, University of Campinas (Unicamp), Campinas, São Paulo, Brazil. Faculty of Pharmaceutical Sciences, University of Campinas (Unicamp), Campinas, São Paulo, Brazil.Federal University of Rio De Janeiro (UFRJ), Macaé University Campus, Rio de Janeiro, Brazil.Genetics and Biochemistry Institute, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil.Dept. of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Science, University of São Paulo (USP), São Paulo, Brazil.School of Chemical Engineering, University of Campinas, Campinas, São Paulo, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25919128

Citation

Spir, Lívia Genovez, et al. "Application of an Aqueous Two-phase Micellar System to Extract Bromelain From Pineapple (Ananas Comosus) Peel Waste and Analysis of Bromelain Stability in Cosmetic Formulations." Biotechnology Progress, vol. 31, no. 4, 2015, pp. 937-45.
Spir LG, Ataide JA, De Lencastre Novaes LC, et al. Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations. Biotechnol Prog. 2015;31(4):937-45.
Spir, L. G., Ataide, J. A., De Lencastre Novaes, L. C., Moriel, P., Mazzola, P. G., De Borba Gurpilhares, D., ... Tambourgi, E. B. (2015). Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations. Biotechnology Progress, 31(4), pp. 937-45. doi:10.1002/btpr.2098.
Spir LG, et al. Application of an Aqueous Two-phase Micellar System to Extract Bromelain From Pineapple (Ananas Comosus) Peel Waste and Analysis of Bromelain Stability in Cosmetic Formulations. Biotechnol Prog. 2015;31(4):937-45. PubMed PMID: 25919128.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations. AU - Spir,Lívia Genovez, AU - Ataide,Janaína Artem, AU - De Lencastre Novaes,Letícia Celia, AU - Moriel,Patrícia, AU - Mazzola,Priscila Gava, AU - De Borba Gurpilhares,Daniela, AU - Silveira,Edgar, AU - Pessoa,Adalberto,Jr AU - Tambourgi,Elias Basile, Y1 - 2015/05/15/ PY - 2014/11/07/received PY - 2015/03/11/revised PY - 2015/4/29/entrez PY - 2015/4/29/pubmed PY - 2016/5/10/medline KW - bromelain KW - cosmetic stability KW - enzyme purification KW - liquid-liquid extraction KW - protein partition SP - 937 EP - 45 JF - Biotechnology progress JO - Biotechnol. Prog. VL - 31 IS - 4 N2 - Bromelain is a set of proteolytic enzymes found in pineapple (Ananas comosus) tissues such as stem, fruit and leaves. Because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. The present study focused on the recovery of bromelain from pineapple peel by liquid-liquid extraction in aqueous two-phase micellar systems (ATPMS), using Triton X-114 (TX-114) and McIlvaine buffer, in the absence and presence of electrolytes CaCl2 and KI; the cloud points of the generated extraction systems were studied by plotting binodal curves. Based on the cloud points, three temperatures were selected for extraction: 30, 33, and 36°C for systems in the absence of salts; 40, 43, and 46°C in the presence of KI; 24, 27, and 30°C in the presence of CaCl2 . Total protein and enzymatic activities were analyzed to monitor bromelain. Employing the ATPMS chosen for extraction (0.5 M KI with 3% TX-114, at pH 6.0, at 40°C), the bromelain extract stability was assessed after incorporation into three cosmetic bases: an anhydrous gel, a cream, and a cream-gel formulation. The cream-gel formulation presented as the most appropriate base to convey bromelain, and its optimal storage conditions were found to be 4.0 ± 0.5°C. The selected ATPMS enabled the extraction of a biomolecule with high added value from waste lined-up in a cosmetic formulation, allowing for exploration of further cosmetic potential. SN - 1520-6033 UR - https://www.unboundmedicine.com/medline/citation/25919128/Application_of_an_aqueous_two_phase_micellar_system_to_extract_bromelain_from_pineapple__Ananas_comosus__peel_waste_and_analysis_of_bromelain_stability_in_cosmetic_formulations_ L2 - https://doi.org/10.1002/btpr.2098 DB - PRIME DP - Unbound Medicine ER -