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Paeonolum protects against MPP(+)-induced neurotoxicity in zebrafish and PC12 cells.
BMC Complement Altern Med. 2015 Apr 29; 15:137.BC

Abstract

BACKGROUND

Parkinson's disease (PD) is the second most common neurodegenerative disease, affecting 2% of the population aged over 65 years old. Mitochondrial defects and oxidative stress actively participate in degeneration of dopaminergic (DA) neurons in PD. Paeonolum, a main component isolated from Moutan cortex, has potent antioxidant ability. Here, we have examined the effects of paeonolum against MPP(+)-induced neurotoxicity in zebrafish and PC12 cells.

METHODS

The overall viability and neurodegeneration of DA neurons was assessed in ETvmat2:green fluorescent protein (GFP) transgenic zebrafish, in which most monoaminergic neurons are labeled by GFP. Damage to PC12 cells was measured using a cell viability assay and assessment of nuclear morphology. Intracellular reactive oxygen species (ROS) and the level of total GSH were assessed. The mitochondrial cell death pathway including mitochondrial membrane potential, cytochrome C release and caspase-3 activity were also examined in PC12 cells.

RESULTS

Paeonolum protected against MPP(+)-induced DA neurodegeneration and locomotor dysfunction in zebrafish in a concentration-dependent manner. Similar neuroprotection was replicated in the PC12 cellular model of MPP(+) toxicity. Paeonolum attenuated MPP(+)-induced intracellular ROS accumulation and restored the level of total GSH in PC12 cells. Furthermore, paeonolum significantly inhibited the mitochondrial cell death pathway induced by MPP(+).

CONCLUSIONS

Collectively, the present study demonstrates that paeonolum protects zebrafish and PC12 cells against MPP(+)-induced neurotoxicity.

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Authors+Show Affiliations

Lu XL
Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. goodxilin@163.com. National Key Clinical Department, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. goodxilin@163.com. Key Discipline of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. goodxilin@163.com.
Lin YH
Department of Clinical Laboratory, Cancer Center of Sun Yat-sen University, Guangzhou, 510060, China. linyh@sysucc.org.cn.
Wu Q
Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. wuqi@medmail.com.cn. National Key Clinical Department, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. wuqi@medmail.com.cn. Key Discipline of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. wuqi@medmail.com.cn.
Su FJ
Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. jisufengjuan@163.com. National Key Clinical Department, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. jisufengjuan@163.com. Key Discipline of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. jisufengjuan@163.com.
Ye CH
Department of Geriatrics, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. ych201176@163.com.
Shi L
Neurosurgery Intensive Care Unit, Department of Critical Care Medicine, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. shilei.victory@163.com.
He BX
Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. 709194054@qq.com. National Key Clinical Department, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. 709194054@qq.com. Key Discipline of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. 709194054@qq.com.
Huang FW
Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. 916888327@qq.com. National Key Clinical Department, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. 916888327@qq.com. Key Discipline of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. 916888327@qq.com.
Pei Z
Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. peizhong@mail.sysu.edu.cn. National Key Clinical Department, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. peizhong@mail.sysu.edu.cn. Key Discipline of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. peizhong@mail.sysu.edu.cn.
Yao XL
Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. liliyao71@163.com. National Key Clinical Department, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. liliyao71@163.com. Key Discipline of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. liliyao71@163.com.

MeSH

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridineAnimalsAntioxidantsCaspase 3Cell DeathCytochromes cDopamineDopaminergic NeuronsHumansMPTP PoisoningMembrane Potential, MitochondrialMitochondriaNeuroprotective AgentsNeurotoxinsOxidative StressPC12 CellsPaeoniaParkinson DiseasePhytotherapyPlant ExtractsRatsReactive Oxygen SpeciesZebrafish

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25925762

Citation

Lu, Xi-Lin, et al. "Paeonolum Protects Against MPP(+)-induced Neurotoxicity in Zebrafish and PC12 Cells." BMC Complementary and Alternative Medicine, vol. 15, 2015, p. 137.
Lu XL, Lin YH, Wu Q, et al. Paeonolum protects against MPP(+)-induced neurotoxicity in zebrafish and PC12 cells. BMC Complement Altern Med. 2015;15:137.
Lu, X. L., Lin, Y. H., Wu, Q., Su, F. J., Ye, C. H., Shi, L., He, B. X., Huang, F. W., Pei, Z., & Yao, X. L. (2015). Paeonolum protects against MPP(+)-induced neurotoxicity in zebrafish and PC12 cells. BMC Complementary and Alternative Medicine, 15, 137. https://doi.org/10.1186/s12906-015-0661-0
Lu XL, et al. Paeonolum Protects Against MPP(+)-induced Neurotoxicity in Zebrafish and PC12 Cells. BMC Complement Altern Med. 2015 Apr 29;15:137. PubMed PMID: 25925762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Paeonolum protects against MPP(+)-induced neurotoxicity in zebrafish and PC12 cells. AU - Lu,Xi-Lin, AU - Lin,Yue-Hao, AU - Wu,Qi, AU - Su,Feng-Juan, AU - Ye,Cheng-Hui, AU - Shi,Lei, AU - He,Bai-Xuan, AU - Huang,Fei-Wen, AU - Pei,Zhong, AU - Yao,Xiao-Li, Y1 - 2015/04/29/ PY - 2014/12/03/received PY - 2015/04/22/accepted PY - 2015/5/1/entrez PY - 2015/5/1/pubmed PY - 2015/12/15/medline SP - 137 EP - 137 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 15 N2 - BACKGROUND: Parkinson's disease (PD) is the second most common neurodegenerative disease, affecting 2% of the population aged over 65 years old. Mitochondrial defects and oxidative stress actively participate in degeneration of dopaminergic (DA) neurons in PD. Paeonolum, a main component isolated from Moutan cortex, has potent antioxidant ability. Here, we have examined the effects of paeonolum against MPP(+)-induced neurotoxicity in zebrafish and PC12 cells. METHODS: The overall viability and neurodegeneration of DA neurons was assessed in ETvmat2:green fluorescent protein (GFP) transgenic zebrafish, in which most monoaminergic neurons are labeled by GFP. Damage to PC12 cells was measured using a cell viability assay and assessment of nuclear morphology. Intracellular reactive oxygen species (ROS) and the level of total GSH were assessed. The mitochondrial cell death pathway including mitochondrial membrane potential, cytochrome C release and caspase-3 activity were also examined in PC12 cells. RESULTS: Paeonolum protected against MPP(+)-induced DA neurodegeneration and locomotor dysfunction in zebrafish in a concentration-dependent manner. Similar neuroprotection was replicated in the PC12 cellular model of MPP(+) toxicity. Paeonolum attenuated MPP(+)-induced intracellular ROS accumulation and restored the level of total GSH in PC12 cells. Furthermore, paeonolum significantly inhibited the mitochondrial cell death pathway induced by MPP(+). CONCLUSIONS: Collectively, the present study demonstrates that paeonolum protects zebrafish and PC12 cells against MPP(+)-induced neurotoxicity. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/25925762/Paeonolum_protects_against_MPP_+__induced_neurotoxicity_in_zebrafish_and_PC12_cells_ DB - PRIME DP - Unbound Medicine ER -