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Sperm DNA fragmentation and standard semen parameters in algerian infertile male partners.
World J Mens Health 2015; 33(1):1-7WJ

Abstract

PURPOSE

To date, standard semen parameters have been the only parameters investigated in sperm samples of infertile men in Algeria. We investigated, for the first time, semen parameters according to sperm DNA fragmentation (SDF) in these subjects.

MATERIALS AND METHODS

SDF was determined by a validated sperm chromatin dispersion test in 26 infertile men. Patients were split into two groups according to the SDF level estimated by the DNA fragmentation index (DFI): the low fragmentation group (LFG; LFG with DFI ≤18%) and high fragmentation group (HFG; HFG with DFI >18%). The standard semen parameters were measured in both groups.

RESULTS

We found that semen concentration and motility were negatively correlated with DFI (r=-0.65, r=-0.45, respectively; p<0.05), while morphology and semen volume were not correlated with it (r=0.24, r=-0.18, respectively; p>0.05). Comparison of the sperm concentration revealed that it was significantly higher in LFG than in HFG (37.57%±13.16% vs. 7.32%±3.59%, respectively; p<0.05), whereas no significant difference was observed regarding sperm motility and morphology.

CONCLUSIONS

Our findings suggest that SDF correlates well with both sperm motility and concentration but not with morphology. Thus, we conclude that SDF evaluation provides additional information regarding sperm quality, and should be used as a complementary test for assessing semen characteristics in infertile males.

Authors+Show Affiliations

Department of Natural and Life Sciences, Faculty of Sciences, Hadj Lakhdar University, Batna, Algeria.Department of Medicine, Faculty of Medicine, Hadj Lakhdar University, Batna, Algeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25927056

Citation

Boushaba, Sarah, and Ghania Belaaloui. "Sperm DNA Fragmentation and Standard Semen Parameters in Algerian Infertile Male Partners." The World Journal of Men's Health, vol. 33, no. 1, 2015, pp. 1-7.
Boushaba S, Belaaloui G. Sperm DNA fragmentation and standard semen parameters in algerian infertile male partners. World J Mens Health. 2015;33(1):1-7.
Boushaba, S., & Belaaloui, G. (2015). Sperm DNA fragmentation and standard semen parameters in algerian infertile male partners. The World Journal of Men's Health, 33(1), pp. 1-7. doi:10.5534/wjmh.2015.33.1.1.
Boushaba S, Belaaloui G. Sperm DNA Fragmentation and Standard Semen Parameters in Algerian Infertile Male Partners. World J Mens Health. 2015;33(1):1-7. PubMed PMID: 25927056.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sperm DNA fragmentation and standard semen parameters in algerian infertile male partners. AU - Boushaba,Sarah, AU - Belaaloui,Ghania, Y1 - 2015/04/23/ PY - 2014/12/18/received PY - 2015/01/10/revised PY - 2015/02/12/accepted PY - 2015/5/1/entrez PY - 2015/5/1/pubmed PY - 2015/5/1/medline KW - DNA fragmentation KW - Semen KW - Semen analysis SP - 1 EP - 7 JF - The world journal of men's health JO - World J Mens Health VL - 33 IS - 1 N2 - PURPOSE: To date, standard semen parameters have been the only parameters investigated in sperm samples of infertile men in Algeria. We investigated, for the first time, semen parameters according to sperm DNA fragmentation (SDF) in these subjects. MATERIALS AND METHODS: SDF was determined by a validated sperm chromatin dispersion test in 26 infertile men. Patients were split into two groups according to the SDF level estimated by the DNA fragmentation index (DFI): the low fragmentation group (LFG; LFG with DFI ≤18%) and high fragmentation group (HFG; HFG with DFI >18%). The standard semen parameters were measured in both groups. RESULTS: We found that semen concentration and motility were negatively correlated with DFI (r=-0.65, r=-0.45, respectively; p<0.05), while morphology and semen volume were not correlated with it (r=0.24, r=-0.18, respectively; p>0.05). Comparison of the sperm concentration revealed that it was significantly higher in LFG than in HFG (37.57%±13.16% vs. 7.32%±3.59%, respectively; p<0.05), whereas no significant difference was observed regarding sperm motility and morphology. CONCLUSIONS: Our findings suggest that SDF correlates well with both sperm motility and concentration but not with morphology. Thus, we conclude that SDF evaluation provides additional information regarding sperm quality, and should be used as a complementary test for assessing semen characteristics in infertile males. SN - 2287-4208 UR - https://www.unboundmedicine.com/medline/citation/25927056/Sperm_DNA_fragmentation_and_standard_semen_parameters_in_algerian_infertile_male_partners_ L2 - https://wjmh.org/DOIx.php?id=10.5534/wjmh.2015.33.1.1 DB - PRIME DP - Unbound Medicine ER -