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Antimicrobial and antioxidant flavonoids from the leaves of Oncoba spinosa Forssk. (Salicaceae).
BMC Complement Altern Med. 2015 Apr 28; 15:134.BC

Abstract

BACKGROUND

Naturally occurring flavonoids have been reported to possess various pharmacological properties. The aim of this study was to evaluate the antimicrobial and antioxidant activities of the MeOH extract and flavonoids from the leaves of Oncoba spinosa, a plant used for the treatment of syphilis, wounds and sexual impotence.

METHODS

The plant extract was prepared by maceration in methanol and sequentially fractionated by column chromatography. The structures of isolated compounds were elucidated on the basis of spectral studies and comparison with published data. The MeOH extract and its isolated compounds were evaluated for their antibacterial and antifungal activities by broth microdilution method. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and trolox equivalent antioxidant capacity (TEAC) assays were used to detect the antioxidant activity. The samples were tested spectrophotometrically for their hemolytic properties against human red blood cells.

RESULTS

The fractionation of the MeOH extract afforded five known flavonoids including kaempferol (1), quercetin (2), apigenin-7-O-β-D-glucuronopyranoside (3), quercetin 3-O-β-D-galactopyranoside (4) and quercetin 3-O-α-L-rhamnopyranosyl (1 → 6) β-D-glucopyranoside (5). The MeOH extract displayed weak to moderate antimicrobial activities (MIC = 256-2048 μg/ml). Quercetin 3-O-α-L-rhamnopyranosyl (1 → 6) β-D-glucopyranoside (5) and quercetin (2) were respectively the most active compounds against bacteria (MIC = 8-64 μg/ml) and fungi (MIC = 64 - 128 μg/ml). These tested samples also showed high radical-scavenging activities (EC50 = 5.08 - 70.56 μg/ml) and gallic acid equivalent antioxidant capacities (TEAC = 53.76 - 89.86 μg/ml) when compared with vitamin C (EC50 = 4.72 μg/ml). The MeOH extract and compounds 2-5 were non-toxic to human red blood cells indicating their high selectivity to be used as antimicrobial and antioxidant drugs.

CONCLUSION

The MeOH extract of O. spinosa as well as compounds 2 - 5 could be a potential source of natural antimicrobial and antioxidant products.

Authors+Show Affiliations

Chemistry Department, University of Dschang, PO Box 67, Dschang, Cameroon. djouossim@yahoo.com.Laboratory of Microbiology and Antimicrobial Substances, Biochemistry Department, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon. jtamokou@yahoo.fr.Chemistry Department, University of Dschang, PO Box 67, Dschang, Cameroon. dngnokam@yahoo.fr.Laboratory of Microbiology and Antimicrobial Substances, Biochemistry Department, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon. jrkuiate@yahoo.com.Chemistry Department, University of Dschang, PO Box 67, Dschang, Cameroon. tapondjou2001@yahoo.fr.Service Commun d'Analyse, Institut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312, Bat. 18 BP 1039, 51687, Reims cedex 2, France. dominique.harakat@univ-reims.fr.Groupe Isolement et Structure, Institut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312, Bat. 18 BP 1039, 51687, Reims cedex 2, France. laurence.voutquenne@univ-reims.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25928352

Citation

Djouossi, Marie Geneviève, et al. "Antimicrobial and Antioxidant Flavonoids From the Leaves of Oncoba Spinosa Forssk. (Salicaceae)." BMC Complementary and Alternative Medicine, vol. 15, 2015, p. 134.
Djouossi MG, Tamokou JD, Ngnokam D, et al. Antimicrobial and antioxidant flavonoids from the leaves of Oncoba spinosa Forssk. (Salicaceae). BMC Complement Altern Med. 2015;15:134.
Djouossi, M. G., Tamokou, J. D., Ngnokam, D., Kuiate, J. R., Tapondjou, L. A., Harakat, D., & Voutquenne-Nazabadioko, L. (2015). Antimicrobial and antioxidant flavonoids from the leaves of Oncoba spinosa Forssk. (Salicaceae). BMC Complementary and Alternative Medicine, 15, 134. https://doi.org/10.1186/s12906-015-0660-1
Djouossi MG, et al. Antimicrobial and Antioxidant Flavonoids From the Leaves of Oncoba Spinosa Forssk. (Salicaceae). BMC Complement Altern Med. 2015 Apr 28;15:134. PubMed PMID: 25928352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial and antioxidant flavonoids from the leaves of Oncoba spinosa Forssk. (Salicaceae). AU - Djouossi,Marie Geneviève, AU - Tamokou,Jean-de-Dieu, AU - Ngnokam,David, AU - Kuiate,Jules-Roger, AU - Tapondjou,Leon Azefack, AU - Harakat,Dominique, AU - Voutquenne-Nazabadioko,Laurence, Y1 - 2015/04/28/ PY - 2014/09/09/received PY - 2015/04/21/accepted PY - 2015/5/1/entrez PY - 2015/5/1/pubmed PY - 2015/12/15/medline SP - 134 EP - 134 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 15 N2 - BACKGROUND: Naturally occurring flavonoids have been reported to possess various pharmacological properties. The aim of this study was to evaluate the antimicrobial and antioxidant activities of the MeOH extract and flavonoids from the leaves of Oncoba spinosa, a plant used for the treatment of syphilis, wounds and sexual impotence. METHODS: The plant extract was prepared by maceration in methanol and sequentially fractionated by column chromatography. The structures of isolated compounds were elucidated on the basis of spectral studies and comparison with published data. The MeOH extract and its isolated compounds were evaluated for their antibacterial and antifungal activities by broth microdilution method. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and trolox equivalent antioxidant capacity (TEAC) assays were used to detect the antioxidant activity. The samples were tested spectrophotometrically for their hemolytic properties against human red blood cells. RESULTS: The fractionation of the MeOH extract afforded five known flavonoids including kaempferol (1), quercetin (2), apigenin-7-O-β-D-glucuronopyranoside (3), quercetin 3-O-β-D-galactopyranoside (4) and quercetin 3-O-α-L-rhamnopyranosyl (1 → 6) β-D-glucopyranoside (5). The MeOH extract displayed weak to moderate antimicrobial activities (MIC = 256-2048 μg/ml). Quercetin 3-O-α-L-rhamnopyranosyl (1 → 6) β-D-glucopyranoside (5) and quercetin (2) were respectively the most active compounds against bacteria (MIC = 8-64 μg/ml) and fungi (MIC = 64 - 128 μg/ml). These tested samples also showed high radical-scavenging activities (EC50 = 5.08 - 70.56 μg/ml) and gallic acid equivalent antioxidant capacities (TEAC = 53.76 - 89.86 μg/ml) when compared with vitamin C (EC50 = 4.72 μg/ml). The MeOH extract and compounds 2-5 were non-toxic to human red blood cells indicating their high selectivity to be used as antimicrobial and antioxidant drugs. CONCLUSION: The MeOH extract of O. spinosa as well as compounds 2 - 5 could be a potential source of natural antimicrobial and antioxidant products. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/25928352/Antimicrobial_and_antioxidant_flavonoids_from_the_leaves_of_Oncoba_spinosa_Forssk___Salicaceae__ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-015-0660-1 DB - PRIME DP - Unbound Medicine ER -