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Inhibition of Ovarian Epithelial Carcinoma Tumorigenesis and Progression by microRNA 106b Mediated through the RhoC Pathway.
PLoS One. 2015; 10(5):e0125714.Plos

Abstract

Epithelial ovarian cancer (EOC) is the most lethal of the gynecological malignancies. Exploring the molecular mechanisms and major factors of invasion and metastasis could have great significance for the treatment and prognosis of EOC. Studies have demonstrated that microRNA 106b (miR-106b) may be a promising therapeutic target for inhibiting breast cancer bone metastasis, but the role of miR-106b in EOC is largely unknown. In this work, miRNA-106b expression was quantified in various ovarian tissues and tumors. Ovarian carcinoma cell lines were transfected with miR-106b, after which, cell phenotype and expression of relevant molecules was assayed. Dual-luciferase reporter assays and xenograft mouse models were also used to investigate miR-106b and its target gene. MiR-106b mRNA expression was found to be significantly higher in normal ovarian tissues and benign tumors than in ovarian carcinomas and borderline tumors (p < 0.01), and was negatively associated with differentiation (Well vs. Por & Mod) and the International Federation of Gynecology and Obstetrics (FIGO) staging (stage I/II vs. stage III/IV) in ovarian carcinoma (p < 0.05). MiR-106b transfection reduced cell proliferation; promoted G1 or S arrest and apoptosis (p < 0.05); suppressed cell migration and invasion (p < 0.05); reduced Ras homolog gene family member C (RhoC), P70 ribosomal S6 kinase (P70S6K), Bcl-xL, Matrix metallopeptidase 2 (MMP2), MMP9 mRNA and protein expression; and induced p53 expression (p < 0.05). Dual-luciferase reporter assays indicated that miR-106b directly targets RhoC by binding its 3'UTR. MiR-106b transfection also suppressed tumor development and RhoC expression in vivo in xenograft mouse models. This is the first demonstration that miR-106b may inhibit tumorigenesis and progression of EOC by targeting RhoC. The involvement of miR-106b-mediated RhoC downregulation in EOC aggression may give extended insights into molecular mechanisms underlying cancer aggression. Approaches aimed at overexpressing miR-106b may serve as promising therapeutic strategies for treating EOC patients.

Authors+Show Affiliations

Department of Gynecology, the First Affiliated Hospital of China Medical University, Shenyang, 110001, P.R. China.Department of Gynecology, the First Affiliated Hospital of China Medical University, Shenyang, 110001, P.R. China.Department of Gynecology, the First Affiliated Hospital of China Medical University, Shenyang, 110001, P.R. China.Department of Gynecology, the First Affiliated Hospital of China Medical University, Shenyang, 110001, P.R. China.Department of Gynecology, the First Affiliated Hospital of China Medical University, Shenyang, 110001, P.R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25933027

Citation

Chen, Shuo, et al. "Inhibition of Ovarian Epithelial Carcinoma Tumorigenesis and Progression By microRNA 106b Mediated Through the RhoC Pathway." PloS One, vol. 10, no. 5, 2015, pp. e0125714.
Chen S, Chen X, Xiu YL, et al. Inhibition of Ovarian Epithelial Carcinoma Tumorigenesis and Progression by microRNA 106b Mediated through the RhoC Pathway. PLoS ONE. 2015;10(5):e0125714.
Chen, S., Chen, X., Xiu, Y. L., Sun, K. X., & Zhao, Y. (2015). Inhibition of Ovarian Epithelial Carcinoma Tumorigenesis and Progression by microRNA 106b Mediated through the RhoC Pathway. PloS One, 10(5), e0125714. https://doi.org/10.1371/journal.pone.0125714
Chen S, et al. Inhibition of Ovarian Epithelial Carcinoma Tumorigenesis and Progression By microRNA 106b Mediated Through the RhoC Pathway. PLoS ONE. 2015;10(5):e0125714. PubMed PMID: 25933027.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of Ovarian Epithelial Carcinoma Tumorigenesis and Progression by microRNA 106b Mediated through the RhoC Pathway. AU - Chen,Shuo, AU - Chen,Xi, AU - Xiu,Yin-Ling, AU - Sun,Kai-Xuan, AU - Zhao,Yang, Y1 - 2015/05/01/ PY - 2014/10/25/received PY - 2015/03/16/accepted PY - 2015/5/2/entrez PY - 2015/5/2/pubmed PY - 2016/2/5/medline SP - e0125714 EP - e0125714 JF - PloS one JO - PLoS ONE VL - 10 IS - 5 N2 - Epithelial ovarian cancer (EOC) is the most lethal of the gynecological malignancies. Exploring the molecular mechanisms and major factors of invasion and metastasis could have great significance for the treatment and prognosis of EOC. Studies have demonstrated that microRNA 106b (miR-106b) may be a promising therapeutic target for inhibiting breast cancer bone metastasis, but the role of miR-106b in EOC is largely unknown. In this work, miRNA-106b expression was quantified in various ovarian tissues and tumors. Ovarian carcinoma cell lines were transfected with miR-106b, after which, cell phenotype and expression of relevant molecules was assayed. Dual-luciferase reporter assays and xenograft mouse models were also used to investigate miR-106b and its target gene. MiR-106b mRNA expression was found to be significantly higher in normal ovarian tissues and benign tumors than in ovarian carcinomas and borderline tumors (p < 0.01), and was negatively associated with differentiation (Well vs. Por & Mod) and the International Federation of Gynecology and Obstetrics (FIGO) staging (stage I/II vs. stage III/IV) in ovarian carcinoma (p < 0.05). MiR-106b transfection reduced cell proliferation; promoted G1 or S arrest and apoptosis (p < 0.05); suppressed cell migration and invasion (p < 0.05); reduced Ras homolog gene family member C (RhoC), P70 ribosomal S6 kinase (P70S6K), Bcl-xL, Matrix metallopeptidase 2 (MMP2), MMP9 mRNA and protein expression; and induced p53 expression (p < 0.05). Dual-luciferase reporter assays indicated that miR-106b directly targets RhoC by binding its 3'UTR. MiR-106b transfection also suppressed tumor development and RhoC expression in vivo in xenograft mouse models. This is the first demonstration that miR-106b may inhibit tumorigenesis and progression of EOC by targeting RhoC. The involvement of miR-106b-mediated RhoC downregulation in EOC aggression may give extended insights into molecular mechanisms underlying cancer aggression. Approaches aimed at overexpressing miR-106b may serve as promising therapeutic strategies for treating EOC patients. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25933027/Inhibition_of_Ovarian_Epithelial_Carcinoma_Tumorigenesis_and_Progression_by_microRNA_106b_Mediated_through_the_RhoC_Pathway_ L2 - http://dx.plos.org/10.1371/journal.pone.0125714 DB - PRIME DP - Unbound Medicine ER -