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Cytotoxic and genotoxic effects of ethanolic extract of Euphorbia hyssopifolia L. on HepG2 cells.
J Ethnopharmacol. 2015 Jul 21; 170:16-9.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Euphorbia hyssopifolia L. is a weed with recognized antimicrobial potential employed in Indian, Asian and Latin-American popular medicine. However, little is known with regard to its toxic potential. The present study aimed to investigate the cytotoxic and genotoxic effects of ethanolic extract of E. hyssopifolia in HepG2 cell culture.

MATERIALS AND METHODS

Phytochemical screening of ethanolic extract was carried out to determine the presence of active secondary plant metabolites. Six concentrations (0.00001, 0.0001, 0.001, 0.01, 0.1 and 1.0mg/mL) of ethanolic extract were tested by the MTT assay to verify cytotoxicity. Then, genotoxic evaluations (alkaline comet assay and cytokinesis-block micronucleus assay - CBMN) were carried out in HepG2 cells with extract concentrations of 0.01, 0.1 and 1.0mg/mL.

RESULTS

Mono and sesquiterpenes, triterpenes and steroids, and flavonoids were the main classes found in the phytochemical screening. Extract concentrations used in the MTT assay showed no cytotoxic activity. On the other hand, genotoxic activity was verified at 0.1 and 1.0mg/mL in the alkaline comet assay. Additionally, the 1.0mg/mL concentration induced severe cell damage leading to death in the CBMN assay, indicating a cytotoxic effect for this concentration in the latter method.

CONCLUSION

The use of E. hyssopifolia extract for medicinal purposes should be avoided, because concentrations above 0.01mg/mL may pose risk to human health due to cytotoxic and/or genotoxic effects.

Authors+Show Affiliations

Federal University of Pernambuco, Centre of Biological Sciences, Genetics Department, 50670-420 Recife, PE, Brazil.São Paulo State University "Júlio de Mesquita Filho", Institut of Biosciences of Rio Claro, Department of Biology, 13506-900 Rio Claro, SP, Brazil.São Paulo State University "Júlio de Mesquita Filho", Institut of Biosciences of Rio Claro, Department of Biology, 13506-900 Rio Claro, SP, Brazil.Federal University of Pernambuco, Centre of Biological Sciences, Genetics Department, 50670-420 Recife, PE, Brazil.São Paulo State University "Júlio de Mesquita Filho", Institut of Biosciences of Rio Claro, Department of Biology, 13506-900 Rio Claro, SP, Brazil.Federal University of Pernambuco, Centre of Health Sciences, Pharmaceutical Sciences Department, 50670-420 Recife, PE, Brazil.Federal University of Pernambuco, Centre of Health Sciences, Pharmaceutical Sciences Department, 50670-420 Recife, PE, Brazil.Federal University of Pernambuco, Centre of Biological Sciences, Genetics Department, 50670-420 Recife, PE, Brazil.Federal University of Pernambuco, Centre of Biological Sciences, Genetics Department, 50670-420 Recife, PE, Brazil. Electronic address: brasileirovidal.ac@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25937254

Citation

Araújo, Silvany de Sousa, et al. "Cytotoxic and Genotoxic Effects of Ethanolic Extract of Euphorbia Hyssopifolia L. On HepG2 Cells." Journal of Ethnopharmacology, vol. 170, 2015, pp. 16-9.
Araújo Sde S, Fernandes TC, Cardona YT, et al. Cytotoxic and genotoxic effects of ethanolic extract of Euphorbia hyssopifolia L. on HepG2 cells. J Ethnopharmacol. 2015;170:16-9.
Araújo, S. d. e. . S., Fernandes, T. C., Cardona, Y. T., Almeida, P. M., Marin-Morales, M. A., Dos Santos, A. V., Randau, K. P., Benko-Iseppon, A. M., & Brasileiro-Vidal, A. C. (2015). Cytotoxic and genotoxic effects of ethanolic extract of Euphorbia hyssopifolia L. on HepG2 cells. Journal of Ethnopharmacology, 170, 16-9. https://doi.org/10.1016/j.jep.2015.04.044
Araújo Sde S, et al. Cytotoxic and Genotoxic Effects of Ethanolic Extract of Euphorbia Hyssopifolia L. On HepG2 Cells. J Ethnopharmacol. 2015 Jul 21;170:16-9. PubMed PMID: 25937254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytotoxic and genotoxic effects of ethanolic extract of Euphorbia hyssopifolia L. on HepG2 cells. AU - Araújo,Silvany de Sousa, AU - Fernandes,Thaís Cristina Casimiro, AU - Cardona,Yaliana Tafurt, AU - Almeida,Pedro Marcos de, AU - Marin-Morales,Maria Aparecida, AU - Dos Santos,Andrea Vidal, AU - Randau,Karina Perrelli, AU - Benko-Iseppon,Ana Maria, AU - Brasileiro-Vidal,Ana Christina, Y1 - 2015/04/30/ PY - 2014/07/22/received PY - 2015/03/02/revised PY - 2015/04/22/accepted PY - 2015/5/5/entrez PY - 2015/5/6/pubmed PY - 2016/3/10/medline KW - Antimicrobial KW - Botany KW - Cell death KW - Comet assay KW - Cytochalasin B (PubChem CID: 5368686) KW - Cytotoxicity KW - Dimethyl Sulfoxide (PubChem CID: 679) KW - Ethanol (PubChem CID: 702) KW - Ethanolic extract KW - Euphorbiaceae KW - Methyl methanesulfonate (PubChem CID: 4156) KW - Micronucleus KW - Mutagenesis KW - Octoxynol (PubChem CID: 5590) KW - Sodium pyruvate (PubChem CID: 23662274) KW - Thiazolyl blue (PubChem CID: 64965) KW - Traditional medicine Africa KW - Traditional medicine meso- and Southern America SP - 16 EP - 9 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 170 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Euphorbia hyssopifolia L. is a weed with recognized antimicrobial potential employed in Indian, Asian and Latin-American popular medicine. However, little is known with regard to its toxic potential. The present study aimed to investigate the cytotoxic and genotoxic effects of ethanolic extract of E. hyssopifolia in HepG2 cell culture. MATERIALS AND METHODS: Phytochemical screening of ethanolic extract was carried out to determine the presence of active secondary plant metabolites. Six concentrations (0.00001, 0.0001, 0.001, 0.01, 0.1 and 1.0mg/mL) of ethanolic extract were tested by the MTT assay to verify cytotoxicity. Then, genotoxic evaluations (alkaline comet assay and cytokinesis-block micronucleus assay - CBMN) were carried out in HepG2 cells with extract concentrations of 0.01, 0.1 and 1.0mg/mL. RESULTS: Mono and sesquiterpenes, triterpenes and steroids, and flavonoids were the main classes found in the phytochemical screening. Extract concentrations used in the MTT assay showed no cytotoxic activity. On the other hand, genotoxic activity was verified at 0.1 and 1.0mg/mL in the alkaline comet assay. Additionally, the 1.0mg/mL concentration induced severe cell damage leading to death in the CBMN assay, indicating a cytotoxic effect for this concentration in the latter method. CONCLUSION: The use of E. hyssopifolia extract for medicinal purposes should be avoided, because concentrations above 0.01mg/mL may pose risk to human health due to cytotoxic and/or genotoxic effects. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/25937254/Cytotoxic_and_genotoxic_effects_of_ethanolic_extract_of_Euphorbia_hyssopifolia_L__on_HepG2_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)00310-4 DB - PRIME DP - Unbound Medicine ER -