Tags

Type your tag names separated by a space and hit enter

A molecular epidemiology of treponemes in beef cattle digital dermatitis lesions and comparative analyses with sheep contagious ovine digital dermatitis and dairy cattle digital dermatitis lesions.
Vet Microbiol. 2015 Jul 09; 178(1-2):77-87.VM

Abstract

Bovine digital dermatitis (BDD) is an infective foot disease commonly reported in dairy cattle where Treponema are considered as the primary causative infectious agents. There still remains little definitive information on the etiology of BDD in beef cattle suggesting further investigations are warranted. Beef BDD lesions (n=34) and healthy beef foot tissues (n=38) were analysed by PCR for three BDD-associated Treponema phylogroups and also for Dichelobacter nodosus and Fusobacterium necrophorum. Spirochete culture was attempted on all BDD lesion samples. One or more BDD-associated Treponema phylogroups were detected in 100% of beef BDD lesions. "Treponema medium/Treponema vincentii-like", "Treponema phagedenis-like" and Treponema pedis spirochetes were identified in 27/34 (79%), 31/34 (91%) and 24/34 (71%) of BDD lesions, respectively. No BDD-associated treponeme DNA was amplified from beef healthy foot tissues. D. nodosus and F. necrophorum were present in 24/34 (71%) and 15/34 (44%) of lesions and 10/38 (26%) and 12/38 (32%) of healthy foot tissues, respectively. Twenty spirochetes were isolated from beef BDD lesions; 19 were representatives of the three BDD-associated Treponema phylogroups. One spirochete isolate shared less than 97% 16S rRNA gene similarity to the three cultivable BDD-associated Treponema phylogroups and therefore may represent a novel taxa of Treponema. Upon comparison, sheep contagious ovine digital dermatitis (CODD), dairy cattle and beef cattle BDD lesions appear to have extremely similar bacteriological data and therefore provides evidence of a shared etiopathogenesis posing concerns for cross-species transmission.

Authors+Show Affiliations

Department of Infection Biology, School of Veterinary Science, Institute of infection and Global Health, Liverpool, Merseyside, L3 5RF, UK. Electronic address: l.sullivan@liverpool.ac.uk.Department of Infection Biology, School of Veterinary Science, Institute of infection and Global Health, Liverpool, Merseyside, L3 5RF, UK.University of Liverpool & Wood Veterinary Group, Gloucester, Gloucestershire, GL2 4NB UK.Department of Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, Neston, Wirral, Cheshire CH64 7TE, UK.Department of Infection Biology, School of Veterinary Science, Institute of infection and Global Health, Liverpool, Merseyside, L3 5RF, UK.Department of Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, Neston, Wirral, Cheshire CH64 7TE, UK.Department of Infection Biology, School of Veterinary Science, Institute of infection and Global Health, Liverpool, Merseyside, L3 5RF, UK.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25937315

Citation

Sullivan, L E., et al. "A Molecular Epidemiology of Treponemes in Beef Cattle Digital Dermatitis Lesions and Comparative Analyses With Sheep Contagious Ovine Digital Dermatitis and Dairy Cattle Digital Dermatitis Lesions." Veterinary Microbiology, vol. 178, no. 1-2, 2015, pp. 77-87.
Sullivan LE, Evans NJ, Blowey RW, et al. A molecular epidemiology of treponemes in beef cattle digital dermatitis lesions and comparative analyses with sheep contagious ovine digital dermatitis and dairy cattle digital dermatitis lesions. Vet Microbiol. 2015;178(1-2):77-87.
Sullivan, L. E., Evans, N. J., Blowey, R. W., Grove-White, D. H., Clegg, S. R., Duncan, J. S., & Carter, S. D. (2015). A molecular epidemiology of treponemes in beef cattle digital dermatitis lesions and comparative analyses with sheep contagious ovine digital dermatitis and dairy cattle digital dermatitis lesions. Veterinary Microbiology, 178(1-2), 77-87. https://doi.org/10.1016/j.vetmic.2015.04.011
Sullivan LE, et al. A Molecular Epidemiology of Treponemes in Beef Cattle Digital Dermatitis Lesions and Comparative Analyses With Sheep Contagious Ovine Digital Dermatitis and Dairy Cattle Digital Dermatitis Lesions. Vet Microbiol. 2015 Jul 9;178(1-2):77-87. PubMed PMID: 25937315.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A molecular epidemiology of treponemes in beef cattle digital dermatitis lesions and comparative analyses with sheep contagious ovine digital dermatitis and dairy cattle digital dermatitis lesions. AU - Sullivan,L E, AU - Evans,N J, AU - Blowey,R W, AU - Grove-White,D H, AU - Clegg,S R, AU - Duncan,J S, AU - Carter,S D, Y1 - 2015/04/21/ PY - 2015/03/18/received PY - 2015/04/08/revised PY - 2015/04/10/accepted PY - 2015/5/5/entrez PY - 2015/5/6/pubmed PY - 2016/2/18/medline KW - Beef cattle KW - Digital dermatitis KW - Lameness KW - Treponemes SP - 77 EP - 87 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 178 IS - 1-2 N2 - Bovine digital dermatitis (BDD) is an infective foot disease commonly reported in dairy cattle where Treponema are considered as the primary causative infectious agents. There still remains little definitive information on the etiology of BDD in beef cattle suggesting further investigations are warranted. Beef BDD lesions (n=34) and healthy beef foot tissues (n=38) were analysed by PCR for three BDD-associated Treponema phylogroups and also for Dichelobacter nodosus and Fusobacterium necrophorum. Spirochete culture was attempted on all BDD lesion samples. One or more BDD-associated Treponema phylogroups were detected in 100% of beef BDD lesions. "Treponema medium/Treponema vincentii-like", "Treponema phagedenis-like" and Treponema pedis spirochetes were identified in 27/34 (79%), 31/34 (91%) and 24/34 (71%) of BDD lesions, respectively. No BDD-associated treponeme DNA was amplified from beef healthy foot tissues. D. nodosus and F. necrophorum were present in 24/34 (71%) and 15/34 (44%) of lesions and 10/38 (26%) and 12/38 (32%) of healthy foot tissues, respectively. Twenty spirochetes were isolated from beef BDD lesions; 19 were representatives of the three BDD-associated Treponema phylogroups. One spirochete isolate shared less than 97% 16S rRNA gene similarity to the three cultivable BDD-associated Treponema phylogroups and therefore may represent a novel taxa of Treponema. Upon comparison, sheep contagious ovine digital dermatitis (CODD), dairy cattle and beef cattle BDD lesions appear to have extremely similar bacteriological data and therefore provides evidence of a shared etiopathogenesis posing concerns for cross-species transmission. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/25937315/A_molecular_epidemiology_of_treponemes_in_beef_cattle_digital_dermatitis_lesions_and_comparative_analyses_with_sheep_contagious_ovine_digital_dermatitis_and_dairy_cattle_digital_dermatitis_lesions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(15)00156-X DB - PRIME DP - Unbound Medicine ER -