TY - JOUR T1 - The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2. AU - Kang,Ji Sook, AU - Choi,Il-Whan, AU - Han,Min Ho, AU - Lee,Dae-Sung, AU - Kim,Gi-Young, AU - Hwang,Hye Jin, AU - Kim,Byung Woo, AU - Kim,Cheol Min, AU - Yoo,Young Hyun, AU - Choi,Yung Hyun, Y1 - 2015/04/29/ PY - 2015/03/13/received PY - 2015/04/08/revised PY - 2015/04/21/accepted PY - 2015/5/5/entrez PY - 2015/5/6/pubmed PY - 2016/1/29/medline SP - 2666 EP - 79 JF - Marine drugs JO - Mar Drugs VL - 13 IS - 5 N2 - This study was designed to examine the protective effects of the marine brown algae Petalonia binghamiae against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms. P. binghamiae methanol extract (PBME) prevented hydrogen peroxide (H2O2)-induced growth inhibition and exhibited scavenging activity against intracellular reactive oxygen species (ROS) induced by H2O2 in mouse-derived C2C12 myoblasts. PBME also significantly attenuated H2O2-induced comet tail formation in a comet assay, histone γH2A.X phosphorylation, and annexin V-positive cells, suggesting that PBME prevented H2O2-induced cellular DNA damage and apoptotic cell death. Furthermore, PBME increased the levels of heme oxygenase-1 (HO-1), a potent antioxidant enzyme, associated with the induction of nuclear factor-erythroid 2 related factor 2 (Nrf2). However, zinc protoporphyrin IX, a HO-1 competitive inhibitor, significantly abolished the protective effects of PBME on H2O2-induced ROS generation, growth inhibition, and apoptosis. Collectively, these results demonstrate that PBME augments the antioxidant defense capacity through activation of the Nrf2/HO-1 pathway. SN - 1660-3397 UR - https://www.unboundmedicine.com/medline/citation/25939035/The_Cytoprotective_Effect_of_Petalonia_binghamiae_Methanol_Extract_against_Oxidative_Stress_in_C2C12_Myoblasts:_Mediation_by_Upregulation_of_Heme_Oxygenase_1_and_Nuclear_Factor_Erythroid_2_Related_Factor_2_ L2 - https://www.mdpi.com/resolver?pii=md13052666 DB - PRIME DP - Unbound Medicine ER -