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Quantification of liensinine in rat plasma using ultra-performance liquid chromatography tandem mass spectrometry and its application to a pharmacokinetic study.

Abstract

An ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine liensinine in rat plasma using carbamazepine as the internal standard (IS). Sample preparation was accomplished through a protein precipitation procedure with acetonitrile to 0.1ml plasma sample. The analyte and IS were separated on an Acquity UPLC BEH C18 column (2.1mm×50mm, 1.7μm) with the mobile phase of acetonitrile and 0.1% formic acid in water with gradient elution at a flow rate of 0.40ml/min. The injection volume was 6μl. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 611.6→206.2 for liensinine and m/z 237.1→194.2 for IS. The linearity of this method was found to be within the concentration range of 10-1000ng/ml with a lower limit of quantification of 10ng/ml. Only 3.0min was needed for an analytical run. The matrix effect was 93.8-107.4% for liensinine. The intra- and inter-day precision (RSD %) were less than 9.9% and accuracy (RE %) was within ±10.5%. The recovery ranged from 76.2 to 86.8%. Liensinine was sufficiently stable under all relevant analytical conditions. The method was also successfully applied to the pharmacokinetic study of liensinine in rats. The pharmacokinetic parameters were demonstrated as followed: t1/2 was 8.2±3.3h, Cmax was 668.4±156.9ng/ml, and AUC0→∞ was 1802.9±466.4ng/mlh.

Authors+Show Affiliations

Luoyang Orthopedic Traumatological Hospital of Henan Province, Henan Province Hospital of orthopedics, Luoyang 471002, China. Electronic address: lvsongfeng88@163.com.Medical College of Henan University of Science and Technology, Luoyang 471003, China.Luoyang Orthopedic Traumatological Hospital of Henan Province, Henan Province Hospital of orthopedics, Luoyang 471002, China.Luoyang Orthopedic Traumatological Hospital of Henan Province, Henan Province Hospital of orthopedics, Luoyang 471002, China.Luoyang Orthopedic Traumatological Hospital of Henan Province, Henan Province Hospital of orthopedics, Luoyang 471002, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25939097

Citation

Lv, Song-Feng, et al. "Quantification of Liensinine in Rat Plasma Using Ultra-performance Liquid Chromatography Tandem Mass Spectrometry and Its Application to a Pharmacokinetic Study." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 992, 2015, pp. 43-6.
Lv SF, Wang XH, Li HW, et al. Quantification of liensinine in rat plasma using ultra-performance liquid chromatography tandem mass spectrometry and its application to a pharmacokinetic study. J Chromatogr B Analyt Technol Biomed Life Sci. 2015;992:43-6.
Lv, S. F., Wang, X. H., Li, H. W., Zhang, X. L., & Wang, B. (2015). Quantification of liensinine in rat plasma using ultra-performance liquid chromatography tandem mass spectrometry and its application to a pharmacokinetic study. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 992, 43-6. https://doi.org/10.1016/j.jchromb.2015.04.023
Lv SF, et al. Quantification of Liensinine in Rat Plasma Using Ultra-performance Liquid Chromatography Tandem Mass Spectrometry and Its Application to a Pharmacokinetic Study. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jun 15;992:43-6. PubMed PMID: 25939097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of liensinine in rat plasma using ultra-performance liquid chromatography tandem mass spectrometry and its application to a pharmacokinetic study. AU - Lv,Song-Feng, AU - Wang,Xiang-hong, AU - Li,Hong-wei, AU - Zhang,Xiao-lei, AU - Wang,Bo, Y1 - 2015/04/20/ PY - 2015/01/28/received PY - 2015/04/10/revised PY - 2015/04/13/accepted PY - 2015/5/5/entrez PY - 2015/5/6/pubmed PY - 2016/2/18/medline KW - Liensinine KW - Pharmacokinetics KW - Rat plasma KW - UPLC–MS/MS SP - 43 EP - 6 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 992 N2 - An ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine liensinine in rat plasma using carbamazepine as the internal standard (IS). Sample preparation was accomplished through a protein precipitation procedure with acetonitrile to 0.1ml plasma sample. The analyte and IS were separated on an Acquity UPLC BEH C18 column (2.1mm×50mm, 1.7μm) with the mobile phase of acetonitrile and 0.1% formic acid in water with gradient elution at a flow rate of 0.40ml/min. The injection volume was 6μl. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 611.6→206.2 for liensinine and m/z 237.1→194.2 for IS. The linearity of this method was found to be within the concentration range of 10-1000ng/ml with a lower limit of quantification of 10ng/ml. Only 3.0min was needed for an analytical run. The matrix effect was 93.8-107.4% for liensinine. The intra- and inter-day precision (RSD %) were less than 9.9% and accuracy (RE %) was within ±10.5%. The recovery ranged from 76.2 to 86.8%. Liensinine was sufficiently stable under all relevant analytical conditions. The method was also successfully applied to the pharmacokinetic study of liensinine in rats. The pharmacokinetic parameters were demonstrated as followed: t1/2 was 8.2±3.3h, Cmax was 668.4±156.9ng/ml, and AUC0→∞ was 1802.9±466.4ng/mlh. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/25939097/Quantification_of_liensinine_in_rat_plasma_using_ultra_performance_liquid_chromatography_tandem_mass_spectrometry_and_its_application_to_a_pharmacokinetic_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(15)00226-3 DB - PRIME DP - Unbound Medicine ER -