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Comparison on the molecular response profiles between nano zinc oxide (ZnO) particles and free zinc ion using a genome-wide toxicogenomics approach.
Environ Sci Pollut Res Int. 2015 Nov; 22(22):17434-42.ES

Abstract

Increasing production and applications of nano zinc oxide particles (nano-ZnO) enhances the probability of its exposure in occupational and environmental settings, but toxicity studies are still limited. Taking the free Zn ion (Zn(2+)) as a control, cytotoxicity of a commercially available nano-ZnO was assessed with a 6-h exposure in Escherichia coli (E. coli). The fitted dose-cytotoxicity curve for ZnCl2 was significantly sharper than that from nano-ZnO. Then, a genome-wide gene expression profile following exposure to nano-ZnO was conducted by use of a live cell reporter assay system with library of 1820 modified green fluorescent protein (GFP)-expressing promoter reporter vectors constructed from E. coli K12 strains, which resulted in 387 significantly altered genes in bacterial (p < 0.001). These altered genes were enriched into ten biological processing and two cell components (p < 0.05) terms through statistical hypergeometric testing, strongly suggesting that exposure to nano-ZnO would result a great disturbance on the functional gene product synthesis processing, such as translation, gene expression, RNA modification, and structural constituent of ribosome. The pattern of expression of 37 genes altered by nano-ZnO (fold change>2) was different from the profile following exposure to 6 mg/L of free zinc ion. The result indicates that these two Zn forms might cause toxicity to bacterial in different modes of action. Our results underscore the importance of understanding the adverse effects elicited by nano-ZnO after entering aquatic environment.

Authors+Show Affiliations

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210089, China.State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210089, China. zhangxw@nju.edu.cn.State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210089, China. Department of Biomedical Veterinary Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, SK, S7N 5B3, Canada. Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloom, Hong Kong, SAR, China.Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210089, China. yuhx@nju.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25940466

Citation

Su, Guanyong, et al. "Comparison On the Molecular Response Profiles Between Nano Zinc Oxide (ZnO) Particles and Free Zinc Ion Using a Genome-wide Toxicogenomics Approach." Environmental Science and Pollution Research International, vol. 22, no. 22, 2015, pp. 17434-42.
Su G, Zhang X, Giesy JP, et al. Comparison on the molecular response profiles between nano zinc oxide (ZnO) particles and free zinc ion using a genome-wide toxicogenomics approach. Environ Sci Pollut Res Int. 2015;22(22):17434-42.
Su, G., Zhang, X., Giesy, J. P., Musarrat, J., Saquib, Q., Alkhedhairy, A. A., & Yu, H. (2015). Comparison on the molecular response profiles between nano zinc oxide (ZnO) particles and free zinc ion using a genome-wide toxicogenomics approach. Environmental Science and Pollution Research International, 22(22), 17434-42. https://doi.org/10.1007/s11356-015-4507-6
Su G, et al. Comparison On the Molecular Response Profiles Between Nano Zinc Oxide (ZnO) Particles and Free Zinc Ion Using a Genome-wide Toxicogenomics Approach. Environ Sci Pollut Res Int. 2015;22(22):17434-42. PubMed PMID: 25940466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison on the molecular response profiles between nano zinc oxide (ZnO) particles and free zinc ion using a genome-wide toxicogenomics approach. AU - Su,Guanyong, AU - Zhang,Xiaowei, AU - Giesy,John P, AU - Musarrat,Javed, AU - Saquib,Quaiser, AU - Alkhedhairy,Abdulaziz A, AU - Yu,Hongxia, Y1 - 2015/05/05/ PY - 2014/08/09/received PY - 2015/04/07/accepted PY - 2015/5/6/entrez PY - 2015/5/6/pubmed PY - 2016/7/12/medline KW - Cytotoxicity KW - Gene expression KW - Gene set enrichment analysis KW - Nanoparticle KW - Pathways SP - 17434 EP - 42 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 22 IS - 22 N2 - Increasing production and applications of nano zinc oxide particles (nano-ZnO) enhances the probability of its exposure in occupational and environmental settings, but toxicity studies are still limited. Taking the free Zn ion (Zn(2+)) as a control, cytotoxicity of a commercially available nano-ZnO was assessed with a 6-h exposure in Escherichia coli (E. coli). The fitted dose-cytotoxicity curve for ZnCl2 was significantly sharper than that from nano-ZnO. Then, a genome-wide gene expression profile following exposure to nano-ZnO was conducted by use of a live cell reporter assay system with library of 1820 modified green fluorescent protein (GFP)-expressing promoter reporter vectors constructed from E. coli K12 strains, which resulted in 387 significantly altered genes in bacterial (p < 0.001). These altered genes were enriched into ten biological processing and two cell components (p < 0.05) terms through statistical hypergeometric testing, strongly suggesting that exposure to nano-ZnO would result a great disturbance on the functional gene product synthesis processing, such as translation, gene expression, RNA modification, and structural constituent of ribosome. The pattern of expression of 37 genes altered by nano-ZnO (fold change>2) was different from the profile following exposure to 6 mg/L of free zinc ion. The result indicates that these two Zn forms might cause toxicity to bacterial in different modes of action. Our results underscore the importance of understanding the adverse effects elicited by nano-ZnO after entering aquatic environment. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/25940466/Comparison_on_the_molecular_response_profiles_between_nano_zinc_oxide__ZnO__particles_and_free_zinc_ion_using_a_genome_wide_toxicogenomics_approach_ DB - PRIME DP - Unbound Medicine ER -