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Microbial community structure in a dual chamber microbial fuel cell fed with brewery waste for azo dye degradation and electricity generation.
Environ Sci Pollut Res Int. 2015 Sep; 22(17):13477-85.ES

Abstract

The expansion in knowledge of the microbial community structure can play a vital role in the electrochemical features and operation of microbial fuel cells (MFCs). In this study, bacterial community composition in a dual chamber MFC fed with brewery waste was investigated for simultaneous electricity generation and azo dye degradation. A stable voltage was generated with a maximum power density of 305 and 269 mW m(-2) for brewery waste alone (2000 mg L(-1)) and after the azo dye (200 mg L(-1)) addition, respectively. Azo dye degradation was confirmed by Fourier transform infrared spectroscopy (FT-IR) as peak corresponding to -N=N- (azo) bond disappeared in the dye metabolites. Microbial communities attached to the anode were analyzed by high-throughput 454 pyrosequencing of the 16S rRNA gene. Microbial community composition analysis revealed that Proteobacteria (67.3 %), Betaproteobacteria (30.8 %), and Desulfovibrio (18.3 %) were the most dominant communities at phylum, class, and genus level, respectively. Among the classified genera, Desulfovibrio most likely plays a major role in electron transfer to the anode since its outer membrane contains c-type cytochromes. At the genus level, 62.3 % of all sequences belonged to the unclassified category indicating a high level of diversity of microbial groups in MFCs fed with brewery waste and azo dye.

HIGHLIGHTS

• Azo dye degradation and stable bioelectricity generation was achieved in the MFC. • Anodic biofilm was analyzed by high-throughput pyrosequencing of the 16S rRNA gene. • Desulfovibrio (18.3 %) was the dominant genus in the classified genera. • Of the genus, 62.3 % were unclassified, thereby indicating highly diverse microbes. Graphical Abstract A schematic diagram of a dual chamber microbial fuel cell for azo dye degradation and current generation (with microbial communities at anode electrode).

Authors+Show Affiliations

Department of Environmental Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 702-701, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25940481

Citation

Miran, Waheed, et al. "Microbial Community Structure in a Dual Chamber Microbial Fuel Cell Fed With Brewery Waste for Azo Dye Degradation and Electricity Generation." Environmental Science and Pollution Research International, vol. 22, no. 17, 2015, pp. 13477-85.
Miran W, Nawaz M, Kadam A, et al. Microbial community structure in a dual chamber microbial fuel cell fed with brewery waste for azo dye degradation and electricity generation. Environ Sci Pollut Res Int. 2015;22(17):13477-85.
Miran, W., Nawaz, M., Kadam, A., Shin, S., Heo, J., Jang, J., & Lee, D. S. (2015). Microbial community structure in a dual chamber microbial fuel cell fed with brewery waste for azo dye degradation and electricity generation. Environmental Science and Pollution Research International, 22(17), 13477-85. https://doi.org/10.1007/s11356-015-4582-8
Miran W, et al. Microbial Community Structure in a Dual Chamber Microbial Fuel Cell Fed With Brewery Waste for Azo Dye Degradation and Electricity Generation. Environ Sci Pollut Res Int. 2015;22(17):13477-85. PubMed PMID: 25940481.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microbial community structure in a dual chamber microbial fuel cell fed with brewery waste for azo dye degradation and electricity generation. AU - Miran,Waheed, AU - Nawaz,Mohsin, AU - Kadam,Avinash, AU - Shin,Seolhye, AU - Heo,Jun, AU - Jang,Jiseon, AU - Lee,Dae Sung, Y1 - 2015/05/05/ PY - 2015/01/28/received PY - 2015/04/21/accepted PY - 2015/5/6/entrez PY - 2015/5/6/pubmed PY - 2016/3/26/medline SP - 13477 EP - 85 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 22 IS - 17 N2 - UNLABELLED: The expansion in knowledge of the microbial community structure can play a vital role in the electrochemical features and operation of microbial fuel cells (MFCs). In this study, bacterial community composition in a dual chamber MFC fed with brewery waste was investigated for simultaneous electricity generation and azo dye degradation. A stable voltage was generated with a maximum power density of 305 and 269 mW m(-2) for brewery waste alone (2000 mg L(-1)) and after the azo dye (200 mg L(-1)) addition, respectively. Azo dye degradation was confirmed by Fourier transform infrared spectroscopy (FT-IR) as peak corresponding to -N=N- (azo) bond disappeared in the dye metabolites. Microbial communities attached to the anode were analyzed by high-throughput 454 pyrosequencing of the 16S rRNA gene. Microbial community composition analysis revealed that Proteobacteria (67.3 %), Betaproteobacteria (30.8 %), and Desulfovibrio (18.3 %) were the most dominant communities at phylum, class, and genus level, respectively. Among the classified genera, Desulfovibrio most likely plays a major role in electron transfer to the anode since its outer membrane contains c-type cytochromes. At the genus level, 62.3 % of all sequences belonged to the unclassified category indicating a high level of diversity of microbial groups in MFCs fed with brewery waste and azo dye. HIGHLIGHTS: • Azo dye degradation and stable bioelectricity generation was achieved in the MFC. • Anodic biofilm was analyzed by high-throughput pyrosequencing of the 16S rRNA gene. • Desulfovibrio (18.3 %) was the dominant genus in the classified genera. • Of the genus, 62.3 % were unclassified, thereby indicating highly diverse microbes. Graphical Abstract A schematic diagram of a dual chamber microbial fuel cell for azo dye degradation and current generation (with microbial communities at anode electrode). SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/25940481/Microbial_community_structure_in_a_dual_chamber_microbial_fuel_cell_fed_with_brewery_waste_for_azo_dye_degradation_and_electricity_generation_ L2 - https://dx.doi.org/10.1007/s11356-015-4582-8 DB - PRIME DP - Unbound Medicine ER -