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ABA Affects Brassinosteroid-Induced Antioxidant Defense via ZmMAP65-1a in Maize Plants.
Plant Cell Physiol. 2015 Jul; 56(7):1442-55.PC

Abstract

Brassinosteroids (BRs) and ABA co-ordinately regulate water deficit tolerance in maize leaves. ZmMAP65-1a, a maize microtubule-associated protein (MAP) which plays an essential role in BR-induced antioxidant defense, has been characterized previously. However, the interactions among BR, ABA and ZmMAP65-1a in water deficit tolerance remain unexplored. In this study, we demonstrated that ABA was required for BR-induced antioxidant defense via ZmMAP65-1a by using biochemical blocking and ABA biosynthetic mutants. The expression of ZmMAP65-1a in maize leaves and mesophyll protoplasts could be increased under polyethylene glycol- (PEG) stimulated water deficit and ABA treatments. Furthermore, the importance of ABA in the early pathway of BR-induced water deficit tolerance was demonstrated by limiting ABA availability. Blocking ABA biosynthesis biochemically or by a null mutation inhibited the downstream gene expression of ZmMAP65-1a and the activity of ZmMAPK5 in the pathway. It also affected the activities of BR-induced antioxidant defense-related enzymes, namely ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), superoxide dismutase (SOD) and NADPH oxidase. In addition, combining results from transiently overexpressed or silenced ZmMAP65-1a in mesophyll protoplasts, we discovered that ZmMAP65-1a mediated the ABA-induced gene expression and activities of APX and SOD. Surprisingly, silencing of ZmMAP65-1a in mesophyll protoplasts did not alter the gene expression of ZmCCaMK and vice versa in response to ABA. Taken together, our data indicate that water deficit-induced ABA is a key mediator in BR-induced antioxidant defense via ZmMAP65-1a in maize.

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Authors+Show Affiliations

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.

Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China ayzhang@njau.edu.

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25941233

Citation

Zhu, Yuan, et al. "ABA Affects Brassinosteroid-Induced Antioxidant Defense Via ZmMAP65-1a in Maize Plants." Plant & Cell Physiology, vol. 56, no. 7, 2015, pp. 1442-55.

Zhu Y, Liu W, Sheng Y, et al. ABA Affects Brassinosteroid-Induced Antioxidant Defense via ZmMAP65-1a in Maize Plants. Plant Cell Physiol. 2015;56(7):1442-55.

Zhu, Y., Liu, W., Sheng, Y., Zhang, J., Chiu, T., Yan, J., Jiang, M., Tan, M., & Zhang, A. (2015). ABA Affects Brassinosteroid-Induced Antioxidant Defense via ZmMAP65-1a in Maize Plants. Plant & Cell Physiology, 56(7), 1442-55. https://doi.org/10.1093/pcp/pcv061

Zhu Y, et al. ABA Affects Brassinosteroid-Induced Antioxidant Defense Via ZmMAP65-1a in Maize Plants. Plant Cell Physiol. 2015;56(7):1442-55. PubMed PMID: 25941233.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - ABA Affects Brassinosteroid-Induced Antioxidant Defense via ZmMAP65-1a in Maize Plants. AU - Zhu,Yuan, AU - Liu,Weijuan, AU - Sheng,Yu, AU - Zhang,Juan, AU - Chiu,Tsanyu, AU - Yan,Jingwei, AU - Jiang,Mingyi, AU - Tan,Mingpu, AU - Zhang,Aying, Y1 - 2015/05/04/ PY - 2014/04/28/received PY - 2015/04/22/accepted PY - 2015/5/6/entrez PY - 2015/5/6/pubmed PY - 2016/4/28/medline KW - ABA KW - Antioxidant defense KW - Brassinosteroid KW - Water deficit KW - ZmMAP65‐1a KW - ZmMAPK5 SP - 1442 EP - 55 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 56 IS - 7 N2 - Brassinosteroids (BRs) and ABA co-ordinately regulate water deficit tolerance in maize leaves. ZmMAP65-1a, a maize microtubule-associated protein (MAP) which plays an essential role in BR-induced antioxidant defense, has been characterized previously. However, the interactions among BR, ABA and ZmMAP65-1a in water deficit tolerance remain unexplored. In this study, we demonstrated that ABA was required for BR-induced antioxidant defense via ZmMAP65-1a by using biochemical blocking and ABA biosynthetic mutants. The expression of ZmMAP65-1a in maize leaves and mesophyll protoplasts could be increased under polyethylene glycol- (PEG) stimulated water deficit and ABA treatments. Furthermore, the importance of ABA in the early pathway of BR-induced water deficit tolerance was demonstrated by limiting ABA availability. Blocking ABA biosynthesis biochemically or by a null mutation inhibited the downstream gene expression of ZmMAP65-1a and the activity of ZmMAPK5 in the pathway. It also affected the activities of BR-induced antioxidant defense-related enzymes, namely ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), superoxide dismutase (SOD) and NADPH oxidase. In addition, combining results from transiently overexpressed or silenced ZmMAP65-1a in mesophyll protoplasts, we discovered that ZmMAP65-1a mediated the ABA-induced gene expression and activities of APX and SOD. Surprisingly, silencing of ZmMAP65-1a in mesophyll protoplasts did not alter the gene expression of ZmCCaMK and vice versa in response to ABA. Taken together, our data indicate that water deficit-induced ABA is a key mediator in BR-induced antioxidant defense via ZmMAP65-1a in maize. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/25941233/ABA_Affects_Brassinosteroid_Induced_Antioxidant_Defense_via_ZmMAP65_1a_in_Maize_Plants_ DB - PRIME DP - Unbound Medicine ER -