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Evaluating diagnostic accuracy of anti-tissue Transglutaminase IgA antibodies as first screening for Celiac Disease in very young children.
Clin Chim Acta. 2015 Jun 15; 446:237-40.CC

Abstract

BACKGROUND

Small bowel biopsy is the gold standard for Celiac Disease (CD) diagnosis, nevertheless serum assays are the first step in ascertaining a diagnosis of CD. New ESPGHAN Criteria 2012 (European Society of Pediatric Gastroenterology Hepatology and Nutrition) suggest using exclusively anti-tissue Transglutaminase IgA antibodies (anti-tTGA) as initial approach to symptomatic subjects. The aim of our study was to evaluate the diagnostic accuracy of anti-tTGA as initial screening assay for CD in a large cohort of pediatric patients.

METHODS

We selected 730 subjects aged between 6 months and 4 years ("Group A") and 348 subjects younger than 2 years (which are part of the 730 subjects) ("Group B"). We performed anti-Deamidated Gliadin Peptides IgA and IgG antibodies (a-DGP IgA/IgG) and anti-tTGA assays by ELISA test. We evaluated the agreement between anti-tTGA and a-DGP IgA/IgG assays and compared the diagnostic accuracy of a-DGP IgA/IgG with that of anti-tTGA in both groups of patients.

RESULTS

There was a substantial agreement between anti-tTGA and a-DGP IgA in "Group A" and an almost perfect agreement in "Group B"; the strength of agreement between anti-tTGA and a-DGP IgG was moderate in "Group A" and substantial in "Group B". anti-tTGA were more sensitive and specific than a-DGP IgA/IgG in both groups.

CONCLUSIONS

anti-tTGA could be used as initial screening assay for CD in all subjects from 6 months of age according to ESPGHAN Criteria 2012.

Authors+Show Affiliations

Department of Molecular Medicine and Medical Biotechnology/DAI of Laboratory Medicine, University of Naples Federico II, Naples, Italy.Department of Molecular Medicine and Medical Biotechnology/DAI of Laboratory Medicine, University of Naples Federico II, Naples, Italy.Department of Molecular Medicine and Medical Biotechnology/DAI of Laboratory Medicine, University of Naples Federico II, Naples, Italy.Department of Molecular Medicine and Medical Biotechnology/DAI of Laboratory Medicine, University of Naples Federico II, Naples, Italy.Department of Translational Medical Science/DAI of Pediatrics, University of Naples Federico II, Naples, Italy.Department of Translational Medical Science/DAI of Pediatrics, University of Naples Federico II, Naples, Italy.Department of Molecular Medicine and Medical Biotechnology/DAI of Laboratory Medicine, University of Naples Federico II, Naples, Italy. Electronic address: caranden@unina.it.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25944768

Citation

Frulio, G, et al. "Evaluating Diagnostic Accuracy of Anti-tissue Transglutaminase IgA Antibodies as First Screening for Celiac Disease in Very Young Children." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 446, 2015, pp. 237-40.
Frulio G, Polimeno A, Palmieri D, et al. Evaluating diagnostic accuracy of anti-tissue Transglutaminase IgA antibodies as first screening for Celiac Disease in very young children. Clin Chim Acta. 2015;446:237-40.
Frulio, G., Polimeno, A., Palmieri, D., Fumi, M., Auricchio, R., Piccolo, E., & Carandente Giarrusso, P. (2015). Evaluating diagnostic accuracy of anti-tissue Transglutaminase IgA antibodies as first screening for Celiac Disease in very young children. Clinica Chimica Acta; International Journal of Clinical Chemistry, 446, 237-40. https://doi.org/10.1016/j.cca.2015.04.035
Frulio G, et al. Evaluating Diagnostic Accuracy of Anti-tissue Transglutaminase IgA Antibodies as First Screening for Celiac Disease in Very Young Children. Clin Chim Acta. 2015 Jun 15;446:237-40. PubMed PMID: 25944768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluating diagnostic accuracy of anti-tissue Transglutaminase IgA antibodies as first screening for Celiac Disease in very young children. AU - Frulio,G, AU - Polimeno,A, AU - Palmieri,D, AU - Fumi,M, AU - Auricchio,R, AU - Piccolo,E, AU - Carandente Giarrusso,P, Y1 - 2015/05/02/ PY - 2014/07/07/received PY - 2015/04/20/revised PY - 2015/04/20/accepted PY - 2015/5/7/entrez PY - 2015/5/7/pubmed PY - 2016/2/24/medline KW - Celiac Disease KW - Children KW - Diagnostic accuracy KW - ELISA KW - anti-Deamidated Gliadin Peptides IgA and IgG antibodies KW - anti-tissue Transglutaminase IgA antibodies SP - 237 EP - 40 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin. Chim. Acta VL - 446 N2 - BACKGROUND: Small bowel biopsy is the gold standard for Celiac Disease (CD) diagnosis, nevertheless serum assays are the first step in ascertaining a diagnosis of CD. New ESPGHAN Criteria 2012 (European Society of Pediatric Gastroenterology Hepatology and Nutrition) suggest using exclusively anti-tissue Transglutaminase IgA antibodies (anti-tTGA) as initial approach to symptomatic subjects. The aim of our study was to evaluate the diagnostic accuracy of anti-tTGA as initial screening assay for CD in a large cohort of pediatric patients. METHODS: We selected 730 subjects aged between 6 months and 4 years ("Group A") and 348 subjects younger than 2 years (which are part of the 730 subjects) ("Group B"). We performed anti-Deamidated Gliadin Peptides IgA and IgG antibodies (a-DGP IgA/IgG) and anti-tTGA assays by ELISA test. We evaluated the agreement between anti-tTGA and a-DGP IgA/IgG assays and compared the diagnostic accuracy of a-DGP IgA/IgG with that of anti-tTGA in both groups of patients. RESULTS: There was a substantial agreement between anti-tTGA and a-DGP IgA in "Group A" and an almost perfect agreement in "Group B"; the strength of agreement between anti-tTGA and a-DGP IgG was moderate in "Group A" and substantial in "Group B". anti-tTGA were more sensitive and specific than a-DGP IgA/IgG in both groups. CONCLUSIONS: anti-tTGA could be used as initial screening assay for CD in all subjects from 6 months of age according to ESPGHAN Criteria 2012. SN - 1873-3492 UR - https://www.unboundmedicine.com/medline/citation/25944768/Evaluating_diagnostic_accuracy_of_anti_tissue_Transglutaminase_IgA_antibodies_as_first_screening_for_Celiac_Disease_in_very_young_children_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-8981(15)00234-X DB - PRIME DP - Unbound Medicine ER -