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Toxoplasma gondii causes death and plastic alteration in the jejunal myenteric plexus.
World J Gastroenterol 2015; 21(16):4829-39WJ

Abstract

AIM

To assess the effects of ME-49 Toxoplasma gondii (T. gondii) strain infection on the myenteric plexus and external muscle of the jejunum in rats.

METHODS

Thirty rats were distributed into two groups: the control group (CG) (n = 15) received 1 mL of saline solution orally, and the infected group (IG) (n = 15) inoculated with 1 mL of saline solution containing 500 oocysts of M-49 T. gondii strain orally. After 36 d of infection, the rats were euthanized. Infection with T. gondii was confirmed by blood samples collected from all rats at the beginning and end of the experiment. The jejunum of five animals was removed and submitted to routine histological processing (paraffin) for analysis of external muscle thickness. The remaining jejunum from the others animals was used to analyze the general population and the NADH-diaphorase, VIPergic and nitrergic subpopulations of myenteric neurons; and the enteric glial cells (S100-IR).

RESULTS

Serological analysis showed that animals from the IG were infected with the parasite. Hypertrophy affecting jejunal muscle thickness was observed in the IG rats (77.02 ± 42.71) in relation to the CG (51.40 ± 12.34), P < 0.05. In addition, 31.2% of the total number of myenteric neurons died (CG: 39839.3 ± 5362.3; IG: 26766.6 ± 2177.6; P < 0.05); hyperplasia of nitrergic myenteric neurons was observed (CG: 7959.0 ± 1290.4; IG: 10893.0 ± 1156.3; P < 0.05); general hypertrophy of the cell body in the remaining myenteric neurons was noted [CG: 232.5 (187.2-286.0); IG: 248.2 (204.4-293.0); P < 0.05]; hypertrophy of the smallest varicosities containing VIP neurotransmitter was seen (CG: 0.46 ± 0.10; IG: 0.80 ± 0.16; P < 0.05) and a reduction of 25.3% in enteric glia cells (CG: 12.64 ± 1.27; IG: 10.09 ± 2.10; P < 0.05) was observed in the infected rats.

CONCLUSION

It was concluded that infection with oocysts of ME-49 T. gondii strain caused quantitative and plastic alterations in the myenteric plexus of the jejunum in rats.

Authors+Show Affiliations

Eduardo José de Almeida Araújo, Departamento de Histologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Paraná, Brazil.Eduardo José de Almeida Araújo, Departamento de Histologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Paraná, Brazil.Eduardo José de Almeida Araújo, Departamento de Histologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Paraná, Brazil.Eduardo José de Almeida Araújo, Departamento de Histologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Paraná, Brazil.Eduardo José de Almeida Araújo, Departamento de Histologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Paraná, Brazil.Eduardo José de Almeida Araújo, Departamento de Histologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Paraná, Brazil.Eduardo José de Almeida Araújo, Departamento de Histologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Paraná, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25944996

Citation

Araújo, Eduardo José de Almeida, et al. "Toxoplasma Gondii Causes Death and Plastic Alteration in the Jejunal Myenteric Plexus." World Journal of Gastroenterology, vol. 21, no. 16, 2015, pp. 4829-39.
Araújo EJ, Zaniolo LM, Vicentino SL, et al. Toxoplasma gondii causes death and plastic alteration in the jejunal myenteric plexus. World J Gastroenterol. 2015;21(16):4829-39.
Araújo, E. J., Zaniolo, L. M., Vicentino, S. L., Góis, M. B., Zanoni, J. N., da Silva, A. V., & Sant'Ana, D. d. e. . M. (2015). Toxoplasma gondii causes death and plastic alteration in the jejunal myenteric plexus. World Journal of Gastroenterology, 21(16), pp. 4829-39. doi:10.3748/wjg.v21.i16.4829.
Araújo EJ, et al. Toxoplasma Gondii Causes Death and Plastic Alteration in the Jejunal Myenteric Plexus. World J Gastroenterol. 2015 Apr 28;21(16):4829-39. PubMed PMID: 25944996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toxoplasma gondii causes death and plastic alteration in the jejunal myenteric plexus. AU - Araújo,Eduardo José de Almeida, AU - Zaniolo,Larissa Marchi, AU - Vicentino,Suellen Laís, AU - Góis,Marcelo Biondaro, AU - Zanoni,Jacqueline Nelisis, AU - da Silva,Aristeu Vieira, AU - Sant'Ana,Débora de Mello Gonçales, PY - 2014/11/07/received PY - 2015/01/01/revised PY - 2015/01/30/accepted PY - 2015/5/7/entrez PY - 2015/5/7/pubmed PY - 2016/1/27/medline KW - Enteric nervous system KW - Glial cells KW - Infectious diseases KW - Neuronal plasticity KW - Nitric oxide KW - Small intestine KW - Toxoplasmosis KW - Vasoactive intestinal peptide SP - 4829 EP - 39 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 21 IS - 16 N2 - AIM: To assess the effects of ME-49 Toxoplasma gondii (T. gondii) strain infection on the myenteric plexus and external muscle of the jejunum in rats. METHODS: Thirty rats were distributed into two groups: the control group (CG) (n = 15) received 1 mL of saline solution orally, and the infected group (IG) (n = 15) inoculated with 1 mL of saline solution containing 500 oocysts of M-49 T. gondii strain orally. After 36 d of infection, the rats were euthanized. Infection with T. gondii was confirmed by blood samples collected from all rats at the beginning and end of the experiment. The jejunum of five animals was removed and submitted to routine histological processing (paraffin) for analysis of external muscle thickness. The remaining jejunum from the others animals was used to analyze the general population and the NADH-diaphorase, VIPergic and nitrergic subpopulations of myenteric neurons; and the enteric glial cells (S100-IR). RESULTS: Serological analysis showed that animals from the IG were infected with the parasite. Hypertrophy affecting jejunal muscle thickness was observed in the IG rats (77.02 ± 42.71) in relation to the CG (51.40 ± 12.34), P < 0.05. In addition, 31.2% of the total number of myenteric neurons died (CG: 39839.3 ± 5362.3; IG: 26766.6 ± 2177.6; P < 0.05); hyperplasia of nitrergic myenteric neurons was observed (CG: 7959.0 ± 1290.4; IG: 10893.0 ± 1156.3; P < 0.05); general hypertrophy of the cell body in the remaining myenteric neurons was noted [CG: 232.5 (187.2-286.0); IG: 248.2 (204.4-293.0); P < 0.05]; hypertrophy of the smallest varicosities containing VIP neurotransmitter was seen (CG: 0.46 ± 0.10; IG: 0.80 ± 0.16; P < 0.05) and a reduction of 25.3% in enteric glia cells (CG: 12.64 ± 1.27; IG: 10.09 ± 2.10; P < 0.05) was observed in the infected rats. CONCLUSION: It was concluded that infection with oocysts of ME-49 T. gondii strain caused quantitative and plastic alterations in the myenteric plexus of the jejunum in rats. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/25944996/Toxoplasma_gondii_causes_death_and_plastic_alteration_in_the_jejunal_myenteric_plexus_ L2 - http://www.wjgnet.com/1007-9327/full/v21/i16/4829.htm DB - PRIME DP - Unbound Medicine ER -