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Comparison of Archaeal and Bacterial Diversity in Methane Seep Carbonate Nodules and Host Sediments, Eel River Basin and Hydrate Ridge, USA.
Microb Ecol 2015; 70(3):766-84ME

Abstract

Anaerobic oxidation of methane (AOM) impacts carbon cycling by acting as a methane sink and by sequestering inorganic carbon via AOM-induced carbonate precipitation. These precipitates commonly take the form of carbonate nodules that form within methane seep sediments. The timing and sequence of nodule formation within methane seep sediments are not well understood. Further, the microbial diversity associated with sediment-hosted nodules has not been well characterized and the degree to which nodules reflect the microbial assemblage in surrounding sediments is unknown. Here, we conducted a comparative study of microbial assemblages in methane-derived authigenic carbonate nodules and their host sediments using molecular, mineralogical, and geochemical methods. Analysis of 16S rRNA gene diversity from paired carbonate nodules and sediments revealed that both sample types contained methanotrophic archaea (ANME-1 and ANME-2) and syntrophic sulfate-reducing bacteria (Desulfobacteraceae and Desulfobulbaceae), as well as other microbial community members. The combination of geochemical and molecular data from Eel River Basin and Hydrate Ridge suggested that some nodules formed in situ and captured the local sediment-hosted microbial community, while other nodules may have been translocated or may represent a record of conditions prior to the contemporary environment. Taken together, this comparative analysis offers clues to the formation regimes and mechanisms of sediment-hosted carbonate nodules.

Authors+Show Affiliations

Department of Earth, Ocean and Atmospheric Science, Florida State University, Tallahassee, FL, 32306, USA. omason@fsu.edu. Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA, 91125, USA. omason@fsu.edu.Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA, 91125, USA. dcase@caltech.edu.Department of Physical and Environmental Sciences, Texas A&M University-Corpus Christi, Corpus Christi, TX, 78412, USA.School of Biological Sciences, Washington State University, Pullman, WA, 99164, USA.US Geological Survey, Menlo Park, CA, 94025, USA.Department of Earth Sciences, University of Minnesota, Minneapolis, MN, 55455, USA.Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA, 91125, USA. vorphan@gps.caltech.edu.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

25947096

Citation

Mason, Olivia U., et al. "Comparison of Archaeal and Bacterial Diversity in Methane Seep Carbonate Nodules and Host Sediments, Eel River Basin and Hydrate Ridge, USA." Microbial Ecology, vol. 70, no. 3, 2015, pp. 766-84.
Mason OU, Case DH, Naehr TH, et al. Comparison of Archaeal and Bacterial Diversity in Methane Seep Carbonate Nodules and Host Sediments, Eel River Basin and Hydrate Ridge, USA. Microb Ecol. 2015;70(3):766-84.
Mason, O. U., Case, D. H., Naehr, T. H., Lee, R. W., Thomas, R. B., Bailey, J. V., & Orphan, V. J. (2015). Comparison of Archaeal and Bacterial Diversity in Methane Seep Carbonate Nodules and Host Sediments, Eel River Basin and Hydrate Ridge, USA. Microbial Ecology, 70(3), pp. 766-84. doi:10.1007/s00248-015-0615-6.
Mason OU, et al. Comparison of Archaeal and Bacterial Diversity in Methane Seep Carbonate Nodules and Host Sediments, Eel River Basin and Hydrate Ridge, USA. Microb Ecol. 2015;70(3):766-84. PubMed PMID: 25947096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of Archaeal and Bacterial Diversity in Methane Seep Carbonate Nodules and Host Sediments, Eel River Basin and Hydrate Ridge, USA. AU - Mason,Olivia U, AU - Case,David H, AU - Naehr,Thomas H, AU - Lee,Raymond W, AU - Thomas,Randal B, AU - Bailey,Jake V, AU - Orphan,Victoria J, Y1 - 2015/05/07/ PY - 2014/11/29/received PY - 2015/04/10/accepted PY - 2015/5/8/entrez PY - 2015/5/8/pubmed PY - 2016/7/15/medline KW - ANME KW - Anaerobic methanotrophs KW - Carbonate concretions KW - Cold seeps KW - Sulfate-reducing bacteria SP - 766 EP - 84 JF - Microbial ecology JO - Microb. Ecol. VL - 70 IS - 3 N2 - Anaerobic oxidation of methane (AOM) impacts carbon cycling by acting as a methane sink and by sequestering inorganic carbon via AOM-induced carbonate precipitation. These precipitates commonly take the form of carbonate nodules that form within methane seep sediments. The timing and sequence of nodule formation within methane seep sediments are not well understood. Further, the microbial diversity associated with sediment-hosted nodules has not been well characterized and the degree to which nodules reflect the microbial assemblage in surrounding sediments is unknown. Here, we conducted a comparative study of microbial assemblages in methane-derived authigenic carbonate nodules and their host sediments using molecular, mineralogical, and geochemical methods. Analysis of 16S rRNA gene diversity from paired carbonate nodules and sediments revealed that both sample types contained methanotrophic archaea (ANME-1 and ANME-2) and syntrophic sulfate-reducing bacteria (Desulfobacteraceae and Desulfobulbaceae), as well as other microbial community members. The combination of geochemical and molecular data from Eel River Basin and Hydrate Ridge suggested that some nodules formed in situ and captured the local sediment-hosted microbial community, while other nodules may have been translocated or may represent a record of conditions prior to the contemporary environment. Taken together, this comparative analysis offers clues to the formation regimes and mechanisms of sediment-hosted carbonate nodules. SN - 1432-184X UR - https://www.unboundmedicine.com/medline/citation/25947096/Comparison_of_Archaeal_and_Bacterial_Diversity_in_Methane_Seep_Carbonate_Nodules_and_Host_Sediments_Eel_River_Basin_and_Hydrate_Ridge_USA_ L2 - https://dx.doi.org/10.1007/s00248-015-0615-6 DB - PRIME DP - Unbound Medicine ER -