Tags

Type your tag names separated by a space and hit enter

Phase behavior of skin lipid mixtures: the effect of cholesterol on lipid organization.
Soft Matter. 2015 Jun 07; 11(21):4326-36.SM

Abstract

The lipid matrix in the stratum corneum (SC), the upper layer of the skin, plays a critical role in the skin barrier. The matrix consists of ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs). In human SC, these lipids form two coexisting crystalline lamellar phases with periodicities of approximately 6 and 13 nm. In the studies reported here, we investigated the effect of CHOL on lipid organization in each of these lamellar phases separately. For this purpose, we used lipid model mixtures. Our studies revealed that CHOL is imperative for the formation of each of the lamellar phases. At low CHOL levels, the formation of the lamellar phases was dramatically changed: a minimum 0.2 CHOL level in the CER/CHOL/FFA (1 : 0.2 : 1) mixture is required for the formation of each of the lamellar phases. Furthermore, CHOL enhances the formation of the highly dense orthorhombic lateral packing. The gradual increment of CHOL increases the fraction of lipids forming the very dense orthorhombic lateral packing. Therefore, these studies demonstrate that CHOL is an indispensable component of the SC lipid matrix and is of fundamental importance for appropriate dense lipid organization and thus important for the skin barrier function.

Authors+Show Affiliations

Leiden Academic Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, Einsteinweg 55, 2333 CC Leiden, The Netherlands. bouwstra@chem.leidenuniv.nl.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25947944

Citation

Mojumdar, E H., et al. "Phase Behavior of Skin Lipid Mixtures: the Effect of Cholesterol On Lipid Organization." Soft Matter, vol. 11, no. 21, 2015, pp. 4326-36.
Mojumdar EH, Gooris GS, Bouwstra JA. Phase behavior of skin lipid mixtures: the effect of cholesterol on lipid organization. Soft Matter. 2015;11(21):4326-36.
Mojumdar, E. H., Gooris, G. S., & Bouwstra, J. A. (2015). Phase behavior of skin lipid mixtures: the effect of cholesterol on lipid organization. Soft Matter, 11(21), 4326-36. https://doi.org/10.1039/c4sm02786h
Mojumdar EH, Gooris GS, Bouwstra JA. Phase Behavior of Skin Lipid Mixtures: the Effect of Cholesterol On Lipid Organization. Soft Matter. 2015 Jun 7;11(21):4326-36. PubMed PMID: 25947944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phase behavior of skin lipid mixtures: the effect of cholesterol on lipid organization. AU - Mojumdar,E H, AU - Gooris,G S, AU - Bouwstra,J A, PY - 2015/5/8/entrez PY - 2015/5/8/pubmed PY - 2016/4/6/medline SP - 4326 EP - 36 JF - Soft matter JO - Soft Matter VL - 11 IS - 21 N2 - The lipid matrix in the stratum corneum (SC), the upper layer of the skin, plays a critical role in the skin barrier. The matrix consists of ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs). In human SC, these lipids form two coexisting crystalline lamellar phases with periodicities of approximately 6 and 13 nm. In the studies reported here, we investigated the effect of CHOL on lipid organization in each of these lamellar phases separately. For this purpose, we used lipid model mixtures. Our studies revealed that CHOL is imperative for the formation of each of the lamellar phases. At low CHOL levels, the formation of the lamellar phases was dramatically changed: a minimum 0.2 CHOL level in the CER/CHOL/FFA (1 : 0.2 : 1) mixture is required for the formation of each of the lamellar phases. Furthermore, CHOL enhances the formation of the highly dense orthorhombic lateral packing. The gradual increment of CHOL increases the fraction of lipids forming the very dense orthorhombic lateral packing. Therefore, these studies demonstrate that CHOL is an indispensable component of the SC lipid matrix and is of fundamental importance for appropriate dense lipid organization and thus important for the skin barrier function. SN - 1744-6848 UR - https://www.unboundmedicine.com/medline/citation/25947944/Phase_behavior_of_skin_lipid_mixtures:_the_effect_of_cholesterol_on_lipid_organization_ L2 - https://doi.org/10.1039/c4sm02786h DB - PRIME DP - Unbound Medicine ER -