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Transcriptome profiling of trichome-less reveals genes associated with multicellular trichome development in Cucumis sativus.
Mol Genet Genomics. 2015 Oct; 290(5):2007-18.MG

Abstract

Trichomes on plants, similar to fine hairs on animal and human bodies, play important roles in plant survival and development. They also represent a useful model for the study of cell differentiation. Although the regulatory gene network of unicellular trichome development in Arabidopsis thaliana has been well studied, the genes that regulate multicellular trichome development remain unclear. We confirmed that Cucumis sativus (cucumber) trichomes are multicellular and unbranched, but identified a spontaneous mutant, trichome-less (tril), which presented a completely glabrous phenotype. We compared the transcriptome profilings of the tril mutant and wild type using the Illumina HiSeq 2000 sequencing technology. A total of 991 genes exhibited differential expression: 518 were up-regulated and 473 were down-regulated. We further identified 62 differentially expressed genes that encoded crucial transcription factors and were subdivided into seven categories: homeodomain, MADS, MYB, and WRKY domains, ethylene-responsive, zinc finger, and other transcription factor genes. We further analyzed the tissue-expression profiles of two candidate genes, GLABRA2-like and ATHB51-like, using qRT-PCR and found that these two genes were specifically expressed in the epidermis and trichomes, respectively. These results and the tril mutant provide useful tools to study the molecular networks associated with multicellular trichome development.

Authors+Show Affiliations

School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.Shanghai Seed Management Station, 628 Wuzhong Road, Minhang District, Shanghai, 201103, China.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China. jspan71@sjtu.edu.cn.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China. cairun@sjtu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25952908

Citation

Zhao, Jun-Long, et al. "Transcriptome Profiling of Trichome-less Reveals Genes Associated With Multicellular Trichome Development in Cucumis Sativus." Molecular Genetics and Genomics : MGG, vol. 290, no. 5, 2015, pp. 2007-18.
Zhao JL, Wang YL, Yao DQ, et al. Transcriptome profiling of trichome-less reveals genes associated with multicellular trichome development in Cucumis sativus. Mol Genet Genomics. 2015;290(5):2007-18.
Zhao, J. L., Wang, Y. L., Yao, D. Q., Zhu, W. Y., Chen, L., He, H. L., Pan, J. S., & Cai, R. (2015). Transcriptome profiling of trichome-less reveals genes associated with multicellular trichome development in Cucumis sativus. Molecular Genetics and Genomics : MGG, 290(5), 2007-18. https://doi.org/10.1007/s00438-015-1057-z
Zhao JL, et al. Transcriptome Profiling of Trichome-less Reveals Genes Associated With Multicellular Trichome Development in Cucumis Sativus. Mol Genet Genomics. 2015;290(5):2007-18. PubMed PMID: 25952908.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome profiling of trichome-less reveals genes associated with multicellular trichome development in Cucumis sativus. AU - Zhao,Jun-Long, AU - Wang,Yun-Li, AU - Yao,Dan-Qing, AU - Zhu,Wen-Ying, AU - Chen,Long, AU - He,Huan-Le, AU - Pan,Jun-Song, AU - Cai,Run, Y1 - 2015/05/08/ PY - 2014/11/12/received PY - 2015/04/22/accepted PY - 2015/5/9/entrez PY - 2015/5/9/pubmed PY - 2015/12/15/medline KW - Cucumis sativus KW - Differential expression KW - Multicellular trichomes KW - Transcription factors KW - Transcriptome SP - 2007 EP - 18 JF - Molecular genetics and genomics : MGG JO - Mol Genet Genomics VL - 290 IS - 5 N2 - Trichomes on plants, similar to fine hairs on animal and human bodies, play important roles in plant survival and development. They also represent a useful model for the study of cell differentiation. Although the regulatory gene network of unicellular trichome development in Arabidopsis thaliana has been well studied, the genes that regulate multicellular trichome development remain unclear. We confirmed that Cucumis sativus (cucumber) trichomes are multicellular and unbranched, but identified a spontaneous mutant, trichome-less (tril), which presented a completely glabrous phenotype. We compared the transcriptome profilings of the tril mutant and wild type using the Illumina HiSeq 2000 sequencing technology. A total of 991 genes exhibited differential expression: 518 were up-regulated and 473 were down-regulated. We further identified 62 differentially expressed genes that encoded crucial transcription factors and were subdivided into seven categories: homeodomain, MADS, MYB, and WRKY domains, ethylene-responsive, zinc finger, and other transcription factor genes. We further analyzed the tissue-expression profiles of two candidate genes, GLABRA2-like and ATHB51-like, using qRT-PCR and found that these two genes were specifically expressed in the epidermis and trichomes, respectively. These results and the tril mutant provide useful tools to study the molecular networks associated with multicellular trichome development. SN - 1617-4623 UR - https://www.unboundmedicine.com/medline/citation/25952908/Transcriptome_profiling_of_trichome_less_reveals_genes_associated_with_multicellular_trichome_development_in_Cucumis_sativus_ L2 - https://doi.org/10.1007/s00438-015-1057-z DB - PRIME DP - Unbound Medicine ER -