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MicroRNA-137 suppresses tumor growth in epithelial ovarian cancer in vitro and in vivo.
Mol Med Rep. 2015 Aug; 12(2):3107-14.MM

Abstract

Epithelial ovarian cancer (EOC) remains a major gynecological problem, with a poor 5-year-survival rate due to distant metastases. The identification of microRNAs (miRNAs) may provide a novel avenue for diagnostic and treatment regimens for EOC. Several miRNAs have been reported to be involved in the progression of EOC, among which miRNA (miR)-137 has been observed to be downregulated in the ovarian tissues of patients with EOC. However, the functions of miR-137 in EOC cell apoptosis, migration and invasion remain to be elucidated. In the present study, the expression of miR-137 was measured in clinical ovarian cancer specimens and cell lines using reverse transcription-quantitative polymerase chain reaction. The role of miR-137 in the growth and survival of the SKOV3 human ovarian cancer cell line was determined using several in vitro approaches and in nude mouse models. The results demonstrated that the expression of miR-137 was downregulated in the ovarian cancer specimens and cell lines. It was also observed that enforced expression of miR-137 in the EOC cell lines decreased cell proliferation, clonogenicity, migration and invasion, and induced G1 arrest and cell apoptosis in vitro. Notably, the enforced expression of miR-137 suppressed tumor growth in the nude mice models. These findings suggested that miR-137 may act as a tumor suppressor and be used as a potential therapeutic agent for the treatment of EOC.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, The Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China.Department of Obstetrics and Gynecology, The Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China.Department of Infectious Diseases, The Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China.Department of Obstetrics and Gynecology, The Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25955305

Citation

Zhang, Liyu, et al. "MicroRNA-137 Suppresses Tumor Growth in Epithelial Ovarian Cancer in Vitro and in Vivo." Molecular Medicine Reports, vol. 12, no. 2, 2015, pp. 3107-14.
Zhang L, Li Z, Gai F, et al. MicroRNA-137 suppresses tumor growth in epithelial ovarian cancer in vitro and in vivo. Mol Med Rep. 2015;12(2):3107-14.
Zhang, L., Li, Z., Gai, F., & Wang, Y. (2015). MicroRNA-137 suppresses tumor growth in epithelial ovarian cancer in vitro and in vivo. Molecular Medicine Reports, 12(2), 3107-14. https://doi.org/10.3892/mmr.2015.3756
Zhang L, et al. MicroRNA-137 Suppresses Tumor Growth in Epithelial Ovarian Cancer in Vitro and in Vivo. Mol Med Rep. 2015;12(2):3107-14. PubMed PMID: 25955305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MicroRNA-137 suppresses tumor growth in epithelial ovarian cancer in vitro and in vivo. AU - Zhang,Liyu, AU - Li,Zhihong, AU - Gai,Fengchun, AU - Wang,Yanping, Y1 - 2015/05/07/ PY - 2014/07/21/received PY - 2015/04/08/accepted PY - 2015/5/9/entrez PY - 2015/5/9/pubmed PY - 2016/3/2/medline SP - 3107 EP - 14 JF - Molecular medicine reports JO - Mol Med Rep VL - 12 IS - 2 N2 - Epithelial ovarian cancer (EOC) remains a major gynecological problem, with a poor 5-year-survival rate due to distant metastases. The identification of microRNAs (miRNAs) may provide a novel avenue for diagnostic and treatment regimens for EOC. Several miRNAs have been reported to be involved in the progression of EOC, among which miRNA (miR)-137 has been observed to be downregulated in the ovarian tissues of patients with EOC. However, the functions of miR-137 in EOC cell apoptosis, migration and invasion remain to be elucidated. In the present study, the expression of miR-137 was measured in clinical ovarian cancer specimens and cell lines using reverse transcription-quantitative polymerase chain reaction. The role of miR-137 in the growth and survival of the SKOV3 human ovarian cancer cell line was determined using several in vitro approaches and in nude mouse models. The results demonstrated that the expression of miR-137 was downregulated in the ovarian cancer specimens and cell lines. It was also observed that enforced expression of miR-137 in the EOC cell lines decreased cell proliferation, clonogenicity, migration and invasion, and induced G1 arrest and cell apoptosis in vitro. Notably, the enforced expression of miR-137 suppressed tumor growth in the nude mice models. These findings suggested that miR-137 may act as a tumor suppressor and be used as a potential therapeutic agent for the treatment of EOC. SN - 1791-3004 UR - https://www.unboundmedicine.com/medline/citation/25955305/MicroRNA_137_suppresses_tumor_growth_in_epithelial_ovarian_cancer_in_vitro_and_in_vivo_ L2 - http://www.spandidos-publications.com/mmr/12/2/3107 DB - PRIME DP - Unbound Medicine ER -