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Comparative fine mapping of the Wax 1 (W1) locus in hexaploid wheat.
Theor Appl Genet. 2015 Aug; 128(8):1595-603.TA

Abstract

KEY MESSAGE

By applying comparative genomics analyses, a high-density genetic linkage map of the Wax 1 (W1) locus was constructed as a framework for map-based cloning. Glaucousness is described as the scattering effect of visible light from wax deposited on the cuticle of plant aerial organs. In wheat, the wax on leaves and stems is mainly controlled by two sets of genes: glaucousness loci (W1 and W2) and non-glaucousness loci (Iw1 and Iw2). Bulked segregant analysis (BSA) and simple sequence repeat (SSR) mapping showed that Wax1 (W1) is located on chromosome arm 2BS between markers Xgwm210 and Xbarc35. By applying comparative genomics analyses, colinearity genomic regions of the W1 locus on wheat 2BS were identified in Brachypodium distachyon chromosome 5, rice chromosome 4 and sorghum chromosome 6, respectively. Four STS markers were developed using the Triticum aestivum cv. Chinese Spring 454 contig sequences and the International Wheat Genome Sequencing Consortium (IWGSC) survey sequences. W1 was mapped into a 0.93 cM genetic interval flanked by markers XWGGC3197 and XWGGC2484, which has synteny with genomic regions of 56.5 kb in Brachypodium, 390 kb in rice and 31.8 kb in sorghum. The fine genetic map can serve as a framework for chromosome landing, physical mapping and map-based cloning of the W1 in wheat.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Lu P
State Key Laboratory for Agrobiotechnology/Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.
Qin J
No affiliation info available
Wang G
No affiliation info available
Wang L
No affiliation info available
Wang Z
No affiliation info available
Wu Q
No affiliation info available
Xie J
No affiliation info available
Liang Y
No affiliation info available
Wang Y
No affiliation info available
Zhang D
No affiliation info available
Sun Q
No affiliation info available
Liu Z
No affiliation info available

MeSH

BrachypodiumBreedingChromosomes, PlantComparative Genomic HybridizationDNA, PlantExpressed Sequence TagsGenes, PlantGenetic LinkageGenetic MarkersMicrosatellite RepeatsPhysical Chromosome MappingPolyploidyTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25957646

Citation

Lu, Ping, et al. "Comparative Fine Mapping of the Wax 1 (W1) Locus in Hexaploid Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 128, no. 8, 2015, pp. 1595-603.
Lu P, Qin J, Wang G, et al. Comparative fine mapping of the Wax 1 (W1) locus in hexaploid wheat. Theor Appl Genet. 2015;128(8):1595-603.
Lu, P., Qin, J., Wang, G., Wang, L., Wang, Z., Wu, Q., Xie, J., Liang, Y., Wang, Y., Zhang, D., Sun, Q., & Liu, Z. (2015). Comparative fine mapping of the Wax 1 (W1) locus in hexaploid wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 128(8), 1595-603. https://doi.org/10.1007/s00122-015-2534-9
Lu P, et al. Comparative Fine Mapping of the Wax 1 (W1) Locus in Hexaploid Wheat. Theor Appl Genet. 2015;128(8):1595-603. PubMed PMID: 25957646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative fine mapping of the Wax 1 (W1) locus in hexaploid wheat. AU - Lu,Ping, AU - Qin,Jinxia, AU - Wang,Guoxin, AU - Wang,Lili, AU - Wang,Zhenzhong, AU - Wu,Qiuhong, AU - Xie,Jingzhong, AU - Liang,Yong, AU - Wang,Yong, AU - Zhang,Deyun, AU - Sun,Qixin, AU - Liu,Zhiyong, Y1 - 2015/05/10/ PY - 2015/01/28/received PY - 2015/05/02/accepted PY - 2015/5/11/entrez PY - 2015/5/11/pubmed PY - 2015/9/19/medline SP - 1595 EP - 603 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 128 IS - 8 N2 - KEY MESSAGE: By applying comparative genomics analyses, a high-density genetic linkage map of the Wax 1 (W1) locus was constructed as a framework for map-based cloning. Glaucousness is described as the scattering effect of visible light from wax deposited on the cuticle of plant aerial organs. In wheat, the wax on leaves and stems is mainly controlled by two sets of genes: glaucousness loci (W1 and W2) and non-glaucousness loci (Iw1 and Iw2). Bulked segregant analysis (BSA) and simple sequence repeat (SSR) mapping showed that Wax1 (W1) is located on chromosome arm 2BS between markers Xgwm210 and Xbarc35. By applying comparative genomics analyses, colinearity genomic regions of the W1 locus on wheat 2BS were identified in Brachypodium distachyon chromosome 5, rice chromosome 4 and sorghum chromosome 6, respectively. Four STS markers were developed using the Triticum aestivum cv. Chinese Spring 454 contig sequences and the International Wheat Genome Sequencing Consortium (IWGSC) survey sequences. W1 was mapped into a 0.93 cM genetic interval flanked by markers XWGGC3197 and XWGGC2484, which has synteny with genomic regions of 56.5 kb in Brachypodium, 390 kb in rice and 31.8 kb in sorghum. The fine genetic map can serve as a framework for chromosome landing, physical mapping and map-based cloning of the W1 in wheat. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/25957646/Comparative_fine_mapping_of_the_Wax_1__W1__locus_in_hexaploid_wheat_ DB - PRIME DP - Unbound Medicine ER -