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Intense Raman scattering on hybrid Au/Ag nanoplatforms for the distinction of MMP-9-digested collagen type-I fiber detection.
Biosens Bioelectron. 2015 Oct 15; 72:61-70.BB

Abstract

Well-ordered Au-nanorod arrays were fabricated using the focused ion beam method (denoted as fibAu_NR). Au or Ag nanoclusters (NCs) of various sizes and dimensions were then deposited on the fibAu_NR arrays using electron beam deposition to improve the surface-enhanced Raman scattering (SERS) effect, which was verified using a low concentration of crystal violet (10(-)(5)M) as the probe molecule. An enhancement factor of 6.92 × 10(8) was obtained for NCsfibAu_NR, which is attributed to the combination of intra-NC and NR localized surface plasmon resonance. When 4-aminobenzenethiol (4-ABT)-coated Au or Ag nanoparticles (NPs) were attached to NCsfibAu_NR, the small gaps between 4-ABT-coated NPs and intra-NCs allowed detection at the single-molecule level. Hotspots formed at the interfaces of NCs/NRs and NPs/NCs at a high density, producing a strong local electromagnetic effect. Raman spectra from as-prepared type I collagen (Col-I) and Ag-NP-coated Col-I fibers on NCsfibAu_NR were compared to determine the quantity of amino acids in their triple helix structure. Various concentrations of matrix-metalloproteinase-9-digested Col-I fibers on NCsfibAu_NR were qualitatively examined at a Raman laser wavelength of 785nm to determine the changes of amino acids in the Col-I fiber structure. The results can be used to monitor the growth of healing Col-I fibers in a micro-environment.

Authors+Show Affiliations

Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan; Center for Micro/Nano Science and Technology, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan. Electronic address: jdliao@mail.ncku.edu.tw.Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25957832

Citation

Sivashanmugan, Kundan, et al. "Intense Raman Scattering On Hybrid Au/Ag Nanoplatforms for the Distinction of MMP-9-digested Collagen type-I Fiber Detection." Biosensors & Bioelectronics, vol. 72, 2015, pp. 61-70.
Sivashanmugan K, Liao JD, Shao PL, et al. Intense Raman scattering on hybrid Au/Ag nanoplatforms for the distinction of MMP-9-digested collagen type-I fiber detection. Biosens Bioelectron. 2015;72:61-70.
Sivashanmugan, K., Liao, J. D., Shao, P. L., Liu, B. H., Tseng, T. Y., & Chang, C. Y. (2015). Intense Raman scattering on hybrid Au/Ag nanoplatforms for the distinction of MMP-9-digested collagen type-I fiber detection. Biosensors & Bioelectronics, 72, 61-70. https://doi.org/10.1016/j.bios.2015.04.091
Sivashanmugan K, et al. Intense Raman Scattering On Hybrid Au/Ag Nanoplatforms for the Distinction of MMP-9-digested Collagen type-I Fiber Detection. Biosens Bioelectron. 2015 Oct 15;72:61-70. PubMed PMID: 25957832.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intense Raman scattering on hybrid Au/Ag nanoplatforms for the distinction of MMP-9-digested collagen type-I fiber detection. AU - Sivashanmugan,Kundan, AU - Liao,Jiunn-Der, AU - Shao,Pei-Lin, AU - Liu,Bernard Haochih, AU - Tseng,Te-Yu, AU - Chang,Chih-Yu, Y1 - 2015/04/29/ PY - 2015/02/12/received PY - 2015/03/28/revised PY - 2015/04/27/accepted PY - 2015/5/11/entrez PY - 2015/5/11/pubmed PY - 2016/3/2/medline KW - 4-aminobenzenethiol KW - Au nanorods KW - Digested type I collagen KW - Nanoclusters KW - Surface-enhanced Raman scattering SP - 61 EP - 70 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 72 N2 - Well-ordered Au-nanorod arrays were fabricated using the focused ion beam method (denoted as fibAu_NR). Au or Ag nanoclusters (NCs) of various sizes and dimensions were then deposited on the fibAu_NR arrays using electron beam deposition to improve the surface-enhanced Raman scattering (SERS) effect, which was verified using a low concentration of crystal violet (10(-)(5)M) as the probe molecule. An enhancement factor of 6.92 × 10(8) was obtained for NCsfibAu_NR, which is attributed to the combination of intra-NC and NR localized surface plasmon resonance. When 4-aminobenzenethiol (4-ABT)-coated Au or Ag nanoparticles (NPs) were attached to NCsfibAu_NR, the small gaps between 4-ABT-coated NPs and intra-NCs allowed detection at the single-molecule level. Hotspots formed at the interfaces of NCs/NRs and NPs/NCs at a high density, producing a strong local electromagnetic effect. Raman spectra from as-prepared type I collagen (Col-I) and Ag-NP-coated Col-I fibers on NCsfibAu_NR were compared to determine the quantity of amino acids in their triple helix structure. Various concentrations of matrix-metalloproteinase-9-digested Col-I fibers on NCsfibAu_NR were qualitatively examined at a Raman laser wavelength of 785nm to determine the changes of amino acids in the Col-I fiber structure. The results can be used to monitor the growth of healing Col-I fibers in a micro-environment. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/25957832/Intense_Raman_scattering_on_hybrid_Au/Ag_nanoplatforms_for_the_distinction_of_MMP_9_digested_collagen_type_I_fiber_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(15)30094-4 DB - PRIME DP - Unbound Medicine ER -