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The use of core-shell high-performance liquid chromatography column technology to improve biogenic amine quantification in wine.
J Sci Food Agric. 2016 Mar 30; 96(5):1556-61.JS

Abstract

BACKGROUND

HPLC column technology has been improved, providing better resolution of closely eluting compounds, better analyte sensitivity, and shorter analysis times. The core-shell technology columns offer a faster analysis through the use of shorter columns without compromising resolution. The aim of this work was to improve the methods for determination of biogenic amines (BAs) in wine using the new HPLC PFP core-shell column technology.

RESULTS

Two different elution programs were designed to quantify BAs with the core-shell PFP column. Program I flow rate was 2 mL min(-1). The total elution time was 10 min. In elution program II, the flow rate was 0.8 mL min(-1) and the total elution time was 25 min. The two elution programs used with the core-shell PFP HPLC column showed differences related mainly to the histamine peak. The chromatograms showed that when a temporary isocratic elution was added in the gradient (program II), the histamine peak was eluted later, causing its isolation, and therefore its quantification was easier.

CONCLUSIONS

Compared to the previous C18 HPLC column for the BAs determination in wine, the main advantage of the presented technique is the reduction of the run times and solvent volumes, and has a better sensitivity and selectivity as peaks are higher and sharper.

Authors+Show Affiliations

ENOLAB, ERI-ISIC BioTecMed/IViSoCa, Universitat de València, Burjassot, E46100, Valencia, Spain.ENOLAB, ERI-ISIC BioTecMed/IViSoCa, Universitat de València, Burjassot, E46100, Valencia, Spain.ENOLAB, ERI-ISIC BioTecMed/IViSoCa, Universitat de València, Burjassot, E46100, Valencia, Spain.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25959778

Citation

Berbegal, Carmen, et al. "The Use of Core-shell High-performance Liquid Chromatography Column Technology to Improve Biogenic Amine Quantification in Wine." Journal of the Science of Food and Agriculture, vol. 96, no. 5, 2016, pp. 1556-61.
Berbegal C, Pardo I, Ferrer S. The use of core-shell high-performance liquid chromatography column technology to improve biogenic amine quantification in wine. J Sci Food Agric. 2016;96(5):1556-61.
Berbegal, C., Pardo, I., & Ferrer, S. (2016). The use of core-shell high-performance liquid chromatography column technology to improve biogenic amine quantification in wine. Journal of the Science of Food and Agriculture, 96(5), 1556-61. https://doi.org/10.1002/jsfa.7249
Berbegal C, Pardo I, Ferrer S. The Use of Core-shell High-performance Liquid Chromatography Column Technology to Improve Biogenic Amine Quantification in Wine. J Sci Food Agric. 2016 Mar 30;96(5):1556-61. PubMed PMID: 25959778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The use of core-shell high-performance liquid chromatography column technology to improve biogenic amine quantification in wine. AU - Berbegal,Carmen, AU - Pardo,Isabel, AU - Ferrer,Sergi, Y1 - 2015/06/30/ PY - 2015/01/19/received PY - 2015/04/29/revised PY - 2015/05/04/accepted PY - 2015/5/12/entrez PY - 2015/5/12/pubmed PY - 2016/12/15/medline KW - biogenic amines (BAs) KW - core-shell column technology KW - high-performance liquid chromatography (HPLC) KW - wine SP - 1556 EP - 61 JF - Journal of the science of food and agriculture JO - J. Sci. Food Agric. VL - 96 IS - 5 N2 - BACKGROUND: HPLC column technology has been improved, providing better resolution of closely eluting compounds, better analyte sensitivity, and shorter analysis times. The core-shell technology columns offer a faster analysis through the use of shorter columns without compromising resolution. The aim of this work was to improve the methods for determination of biogenic amines (BAs) in wine using the new HPLC PFP core-shell column technology. RESULTS: Two different elution programs were designed to quantify BAs with the core-shell PFP column. Program I flow rate was 2 mL min(-1). The total elution time was 10 min. In elution program II, the flow rate was 0.8 mL min(-1) and the total elution time was 25 min. The two elution programs used with the core-shell PFP HPLC column showed differences related mainly to the histamine peak. The chromatograms showed that when a temporary isocratic elution was added in the gradient (program II), the histamine peak was eluted later, causing its isolation, and therefore its quantification was easier. CONCLUSIONS: Compared to the previous C18 HPLC column for the BAs determination in wine, the main advantage of the presented technique is the reduction of the run times and solvent volumes, and has a better sensitivity and selectivity as peaks are higher and sharper. SN - 1097-0010 UR - https://www.unboundmedicine.com/medline/citation/25959778/The_use_of_core_shell_high_performance_liquid_chromatography_column_technology_to_improve_biogenic_amine_quantification_in_wine_ L2 - https://doi.org/10.1002/jsfa.7249 DB - PRIME DP - Unbound Medicine ER -