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Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae).
Ecotoxicol Environ Saf. 2015 Sep; 119:15-24.EE

Abstract

Acute lethal and sublethal toxicity of the imidazolinone imazethapyr (IMZT)-based commercial formulation herbicide Pivot H® (10.59% IMZT) was evaluated on Hypsiboas pulchellus tadpoles. Whereas mortality was used as the end point for lethality, frequency of micronuclei (MNs) and other nuclear abnormalities as well as DNA single-strand breaks evaluated by the single cell gel electrophoresis assay were employed to test genotoxicity. Behavioral, growth, developmental, and morphological abnormalities were also employed as sublethal end points. Mortality studies revealed equivalent LC50 (96h) values of 1.49mg/L (confidence limit, 1.09-1.63) and 1.55mg/L (confidence limit, 1.51-1.60) IMZT for Gosner stage (GS) 25 and GS36, respectively. Behavioral changes, i.e., irregular swimming and immobility, as well as a decreased frequency of keratodonts were observed. The herbicide increased the frequency of MNs in circulating erythrocytes of tadpoles exposed for 48h to the highest concentration assayed (1.17mg/L). However, regardless of the concentration of the herbicide assayed, an enhanced frequency of MNs was observed in tadpoles exposed for 96h. The herbicide was able to induce other nuclear abnormalities, i.e., blebbed and notched nuclei, only when tadpoles were exposed for 96h. In addition, we observed that exposure to IMZT within the 0.39-1.17mg/L range increased the genetic damage index in treatments lasting for both 48 and 96h. This study represents the first evidence of acute lethal and sublethal effects exerted by IMZT on amphibians. Finally, our findings highlight the properties of this herbicide that jeopardize nontarget living species exposed to IMZT.

Authors+Show Affiliations

Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 N° 3, B1904AMA La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 N° 3, B1904AMA La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 N° 3, B1904AMA La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.Centro de Investigaciones del Medio Ambiente (CIMA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 N° 3, B1904AMA La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina. Electronic address: marcelo.larramendy@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25966333

Citation

Pérez-Iglesias, J M., et al. "Toxic and Genotoxic Effects of the Imazethapyr-based Herbicide Formulation Pivot H® On Montevideo Tree Frog Hypsiboas Pulchellus Tadpoles (Anura, Hylidae)." Ecotoxicology and Environmental Safety, vol. 119, 2015, pp. 15-24.
Pérez-Iglesias JM, Soloneski S, Nikoloff N, et al. Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae). Ecotoxicol Environ Saf. 2015;119:15-24.
Pérez-Iglesias, J. M., Soloneski, S., Nikoloff, N., Natale, G. S., & Larramendy, M. L. (2015). Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae). Ecotoxicology and Environmental Safety, 119, 15-24. https://doi.org/10.1016/j.ecoenv.2015.04.045
Pérez-Iglesias JM, et al. Toxic and Genotoxic Effects of the Imazethapyr-based Herbicide Formulation Pivot H® On Montevideo Tree Frog Hypsiboas Pulchellus Tadpoles (Anura, Hylidae). Ecotoxicol Environ Saf. 2015;119:15-24. PubMed PMID: 25966333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae). AU - Pérez-Iglesias,J M, AU - Soloneski,S, AU - Nikoloff,N, AU - Natale,G S, AU - Larramendy,M L, Y1 - 2015/05/14/ PY - 2014/12/29/received PY - 2015/04/24/revised PY - 2015/04/28/accepted PY - 2015/5/13/entrez PY - 2015/5/13/pubmed PY - 2016/2/5/medline KW - Imazethapyr-based herbicide formulation KW - Lethality KW - Micronuclei KW - Pivot H® KW - SCGE assay KW - Sublethal effects SP - 15 EP - 24 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 119 N2 - Acute lethal and sublethal toxicity of the imidazolinone imazethapyr (IMZT)-based commercial formulation herbicide Pivot H® (10.59% IMZT) was evaluated on Hypsiboas pulchellus tadpoles. Whereas mortality was used as the end point for lethality, frequency of micronuclei (MNs) and other nuclear abnormalities as well as DNA single-strand breaks evaluated by the single cell gel electrophoresis assay were employed to test genotoxicity. Behavioral, growth, developmental, and morphological abnormalities were also employed as sublethal end points. Mortality studies revealed equivalent LC50 (96h) values of 1.49mg/L (confidence limit, 1.09-1.63) and 1.55mg/L (confidence limit, 1.51-1.60) IMZT for Gosner stage (GS) 25 and GS36, respectively. Behavioral changes, i.e., irregular swimming and immobility, as well as a decreased frequency of keratodonts were observed. The herbicide increased the frequency of MNs in circulating erythrocytes of tadpoles exposed for 48h to the highest concentration assayed (1.17mg/L). However, regardless of the concentration of the herbicide assayed, an enhanced frequency of MNs was observed in tadpoles exposed for 96h. The herbicide was able to induce other nuclear abnormalities, i.e., blebbed and notched nuclei, only when tadpoles were exposed for 96h. In addition, we observed that exposure to IMZT within the 0.39-1.17mg/L range increased the genetic damage index in treatments lasting for both 48 and 96h. This study represents the first evidence of acute lethal and sublethal effects exerted by IMZT on amphibians. Finally, our findings highlight the properties of this herbicide that jeopardize nontarget living species exposed to IMZT. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/25966333/Toxic_and_genotoxic_effects_of_the_imazethapyr_based_herbicide_formulation_Pivot_H®_on_montevideo_tree_frog_Hypsiboas_pulchellus_tadpoles__Anura_Hylidae__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(15)00202-X DB - PRIME DP - Unbound Medicine ER -