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Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites.
Talanta. 2015 Aug 15; 141:247-52.T

Abstract

A label-free and amplified electrochemical impedimetric aptasensor based on functionalized graphene nanocomposites (rGO-AuNPs) was developed for the detection of thrombin, which played a vital role in thrombosis and hemostasis. The thiolated aptamer and dithiothreitol (TBA15-DTT) were firstly immobilized on the gold electrode to capture the thrombin molecules, and then aptamer functionalized graphene nanocomposites (rGO-TBA29) were used to fabricate a sandwich sensing platform for amplifying the impedimetric signals. As numerous negative charges of TBA29 on the electrode repelled to the [Fe(CN)6](4-/3-) anions, resulting in an obvious amplified charge-transfer resistance (Rct) signal. The Rct increase was linearly proportional to the thrombin concentration from 0.3 to 50nM and a detection limit of 0.01nM thrombin was achieved. In addition, graphene could also be labeled with other probes via electrostatic or π-π stacking interactions to produce signals, therefore different detection methods expanding wide application could be used in this model.

Authors+Show Affiliations

College of Chemistry, Jilin University, Changchun 130012, PR China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.College of Chemistry, Jilin University, Changchun 130012, PR China. Electronic address: zzq@jlu.edu.cn.State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China. Electronic address: dongsj@ciac.ac.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25966410

Citation

Wang, Qingqing, et al. "Label-free Aptamer Biosensor for Thrombin Detection Based On Functionalized Graphene Nanocomposites." Talanta, vol. 141, 2015, pp. 247-52.
Wang Q, Zhou Z, Zhai Y, et al. Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites. Talanta. 2015;141:247-52.
Wang, Q., Zhou, Z., Zhai, Y., Zhang, L., Hong, W., Zhang, Z., & Dong, S. (2015). Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites. Talanta, 141, 247-52. https://doi.org/10.1016/j.talanta.2015.04.012
Wang Q, et al. Label-free Aptamer Biosensor for Thrombin Detection Based On Functionalized Graphene Nanocomposites. Talanta. 2015 Aug 15;141:247-52. PubMed PMID: 25966410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites. AU - Wang,Qingqing, AU - Zhou,Zhixue, AU - Zhai,Yanling, AU - Zhang,Lingling, AU - Hong,Wei, AU - Zhang,Zhiquan, AU - Dong,Shaojun, Y1 - 2015/04/13/ PY - 2014/12/16/received PY - 2015/04/01/revised PY - 2015/04/05/accepted PY - 2015/5/13/entrez PY - 2015/5/13/pubmed PY - 2016/2/18/medline KW - Aptasensor KW - Graphene KW - Impedance spectroscopy KW - Nanocomposite KW - Thrombin SP - 247 EP - 52 JF - Talanta JO - Talanta VL - 141 N2 - A label-free and amplified electrochemical impedimetric aptasensor based on functionalized graphene nanocomposites (rGO-AuNPs) was developed for the detection of thrombin, which played a vital role in thrombosis and hemostasis. The thiolated aptamer and dithiothreitol (TBA15-DTT) were firstly immobilized on the gold electrode to capture the thrombin molecules, and then aptamer functionalized graphene nanocomposites (rGO-TBA29) were used to fabricate a sandwich sensing platform for amplifying the impedimetric signals. As numerous negative charges of TBA29 on the electrode repelled to the [Fe(CN)6](4-/3-) anions, resulting in an obvious amplified charge-transfer resistance (Rct) signal. The Rct increase was linearly proportional to the thrombin concentration from 0.3 to 50nM and a detection limit of 0.01nM thrombin was achieved. In addition, graphene could also be labeled with other probes via electrostatic or π-π stacking interactions to produce signals, therefore different detection methods expanding wide application could be used in this model. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/25966410/Label_free_aptamer_biosensor_for_thrombin_detection_based_on_functionalized_graphene_nanocomposites_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(15)00250-7 DB - PRIME DP - Unbound Medicine ER -