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Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites.
Talanta. 2015 Aug 15; 141:247-52.T

Abstract

A label-free and amplified electrochemical impedimetric aptasensor based on functionalized graphene nanocomposites (rGO-AuNPs) was developed for the detection of thrombin, which played a vital role in thrombosis and hemostasis. The thiolated aptamer and dithiothreitol (TBA15-DTT) were firstly immobilized on the gold electrode to capture the thrombin molecules, and then aptamer functionalized graphene nanocomposites (rGO-TBA29) were used to fabricate a sandwich sensing platform for amplifying the impedimetric signals. As numerous negative charges of TBA29 on the electrode repelled to the [Fe(CN)6](4-/3-) anions, resulting in an obvious amplified charge-transfer resistance (Rct) signal. The Rct increase was linearly proportional to the thrombin concentration from 0.3 to 50nM and a detection limit of 0.01nM thrombin was achieved. In addition, graphene could also be labeled with other probes via electrostatic or π-π stacking interactions to produce signals, therefore different detection methods expanding wide application could be used in this model.

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Authors+Show Affiliations

Wang Q
College of Chemistry, Jilin University, Changchun 130012, PR China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Zhou Z
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Zhai Y
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Zhang L
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Hong W
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Zhang Z
College of Chemistry, Jilin University, Changchun 130012, PR China. Electronic address: zzq@jlu.edu.cn.
Dong S
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China. Electronic address: dongsj@ciac.ac.cn.

MeSH

AnimalsAptamers, NucleotideBiosensing TechniquesCattleDielectric SpectroscopyElectrodesGraphiteLimit of DetectionNanocompositesNanoparticlesSerumThrombin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25966410

Citation

Wang, Qingqing, et al. "Label-free Aptamer Biosensor for Thrombin Detection Based On Functionalized Graphene Nanocomposites." Talanta, vol. 141, 2015, pp. 247-52.
Wang Q, Zhou Z, Zhai Y, et al. Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites. Talanta. 2015;141:247-52.
Wang, Q., Zhou, Z., Zhai, Y., Zhang, L., Hong, W., Zhang, Z., & Dong, S. (2015). Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites. Talanta, 141, 247-52. https://doi.org/10.1016/j.talanta.2015.04.012
Wang Q, et al. Label-free Aptamer Biosensor for Thrombin Detection Based On Functionalized Graphene Nanocomposites. Talanta. 2015 Aug 15;141:247-52. PubMed PMID: 25966410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites. AU - Wang,Qingqing, AU - Zhou,Zhixue, AU - Zhai,Yanling, AU - Zhang,Lingling, AU - Hong,Wei, AU - Zhang,Zhiquan, AU - Dong,Shaojun, Y1 - 2015/04/13/ PY - 2014/12/16/received PY - 2015/04/01/revised PY - 2015/04/05/accepted PY - 2015/5/13/entrez PY - 2015/5/13/pubmed PY - 2016/2/18/medline KW - Aptasensor KW - Graphene KW - Impedance spectroscopy KW - Nanocomposite KW - Thrombin SP - 247 EP - 52 JF - Talanta JO - Talanta VL - 141 N2 - A label-free and amplified electrochemical impedimetric aptasensor based on functionalized graphene nanocomposites (rGO-AuNPs) was developed for the detection of thrombin, which played a vital role in thrombosis and hemostasis. The thiolated aptamer and dithiothreitol (TBA15-DTT) were firstly immobilized on the gold electrode to capture the thrombin molecules, and then aptamer functionalized graphene nanocomposites (rGO-TBA29) were used to fabricate a sandwich sensing platform for amplifying the impedimetric signals. As numerous negative charges of TBA29 on the electrode repelled to the [Fe(CN)6](4-/3-) anions, resulting in an obvious amplified charge-transfer resistance (Rct) signal. The Rct increase was linearly proportional to the thrombin concentration from 0.3 to 50nM and a detection limit of 0.01nM thrombin was achieved. In addition, graphene could also be labeled with other probes via electrostatic or π-π stacking interactions to produce signals, therefore different detection methods expanding wide application could be used in this model. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/25966410/Label_free_aptamer_biosensor_for_thrombin_detection_based_on_functionalized_graphene_nanocomposites_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(15)00250-7 DB - PRIME DP - Unbound Medicine ER -