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Quality evaluation and pattern recognition analyses of bioactive marker compounds from Farfarae Flos using HPLC/PDA.
Chem Pharm Bull (Tokyo). 2015; 63(7):546-53.CP

Abstract

The flower bud of Tussilago farfara L., called Farfarae Flos, has traditionally been used in Oriental medicine for the treatment of bronchitis and asthma. To establish a standard for quality control as well as the reliable identification of Farfarae Flos, the contents of five standards, rutin (1), isoquercetin (2), 3,5-dicaffeoylquinic acid (3), tussilagone (4), and tussilagonone (5), were determined by quantitative high-performance liquid chromatography (HPLC)/photodiode array (PDA) analysis. The five standards were separated on a YoungJinBioChrom Aegispak C18-L (250-mm×4.6-mm, 5-µm) column by gradient elution using 0.03% trifluoroacetic acid in water (A), with acetonitrile (B) as the mobile phase. The flow rate was 1.0 mL/min, and the UV detector wavelength was set at 220 nm. The method was successfully used in the analysis of Farfarae Flos from different geographic origins with relatively simple conditions and procedures, and the results demonstrated satisfactory linearity, recovery, precision, accuracy, stability, and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of 62 Farfarae Flos samples. This result indicated that the established HPLC/PDA method is suitable for quantitation and pattern recognition analyses for the quality evaluation of Farfarae Flos.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Seo UM
College of Pharmacy, Catholic University of Daegu.
Zhao BT
No affiliation info available
Kim WI
No affiliation info available
Seo EK
No affiliation info available
Lee JH
No affiliation info available
Min BS
No affiliation info available
Shin BS
No affiliation info available
Son JK
No affiliation info available
Woo MH
No affiliation info available

MeSH

Chromatography, High Pressure LiquidCluster AnalysisFlowersPlant ExtractsQuality ControlTussilago

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25971744

Citation

Seo, U Min, et al. "Quality Evaluation and Pattern Recognition Analyses of Bioactive Marker Compounds From Farfarae Flos Using HPLC/PDA." Chemical & Pharmaceutical Bulletin, vol. 63, no. 7, 2015, pp. 546-53.
Seo UM, Zhao BT, Kim WI, et al. Quality evaluation and pattern recognition analyses of bioactive marker compounds from Farfarae Flos using HPLC/PDA. Chem Pharm Bull (Tokyo). 2015;63(7):546-53.
Seo, U. M., Zhao, B. T., Kim, W. I., Seo, E. K., Lee, J. H., Min, B. S., Shin, B. S., Son, J. K., & Woo, M. H. (2015). Quality evaluation and pattern recognition analyses of bioactive marker compounds from Farfarae Flos using HPLC/PDA. Chemical & Pharmaceutical Bulletin, 63(7), 546-53. https://doi.org/10.1248/cpb.c15-00204
Seo UM, et al. Quality Evaluation and Pattern Recognition Analyses of Bioactive Marker Compounds From Farfarae Flos Using HPLC/PDA. Chem Pharm Bull (Tokyo). 2015;63(7):546-53. PubMed PMID: 25971744.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quality evaluation and pattern recognition analyses of bioactive marker compounds from Farfarae Flos using HPLC/PDA. AU - Seo,U Min, AU - Zhao,Bing Tian, AU - Kim,Won Il, AU - Seo,Eun Kyoung, AU - Lee,Jae Hyun, AU - Min,Byung Sun, AU - Shin,Beom Soo, AU - Son,Jong Keun, AU - Woo,Mi Hee, Y1 - 2015/05/14/ PY - 2015/5/15/entrez PY - 2015/5/15/pubmed PY - 2016/4/9/medline SP - 546 EP - 53 JF - Chemical & pharmaceutical bulletin JO - Chem Pharm Bull (Tokyo) VL - 63 IS - 7 N2 - The flower bud of Tussilago farfara L., called Farfarae Flos, has traditionally been used in Oriental medicine for the treatment of bronchitis and asthma. To establish a standard for quality control as well as the reliable identification of Farfarae Flos, the contents of five standards, rutin (1), isoquercetin (2), 3,5-dicaffeoylquinic acid (3), tussilagone (4), and tussilagonone (5), were determined by quantitative high-performance liquid chromatography (HPLC)/photodiode array (PDA) analysis. The five standards were separated on a YoungJinBioChrom Aegispak C18-L (250-mm×4.6-mm, 5-µm) column by gradient elution using 0.03% trifluoroacetic acid in water (A), with acetonitrile (B) as the mobile phase. The flow rate was 1.0 mL/min, and the UV detector wavelength was set at 220 nm. The method was successfully used in the analysis of Farfarae Flos from different geographic origins with relatively simple conditions and procedures, and the results demonstrated satisfactory linearity, recovery, precision, accuracy, stability, and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of 62 Farfarae Flos samples. This result indicated that the established HPLC/PDA method is suitable for quantitation and pattern recognition analyses for the quality evaluation of Farfarae Flos. SN - 1347-5223 UR - https://www.unboundmedicine.com/medline/citation/25971744/Quality_evaluation_and_pattern_recognition_analyses_of_bioactive_marker_compounds_from_Farfarae_Flos_using_HPLC/PDA_ DB - PRIME DP - Unbound Medicine ER -