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Baculoviral p94 homologs encoded in Cotesia plutellae bracovirus suppress both immunity and development of the diamondback moth, Plutellae xylostella.
Insect Sci. 2016 Apr; 23(2):235-44.IS

Abstract

Polydnaviruses (PDVs) are a group of insect DNA viruses, which exhibit a mutual symbiotic relationship with their specific host wasps. Moreover, most encapsidated genes identified so far in PDVs share homologies with insect-originated genes, but not with virus-originated genes. In the meantime, PDVs associated with 2 wasp genera Cotesia and Glytapanteles encode some genes presumably originated from other viruses. Cotesia plutellae bracovirus (CpBV) encodes 4 genes homologous to baculoviral p94: CpBV-E94k1, CpBV-E94k2, CpBV-E94k3, and CpBV-E94k4. This study was conducted to predict the origin of CpBV-E94ks by comparing their sequences with those of baculoviral orthologs and to determine the physiological functions by their transient expressions in nonparasitized larvae and subsequent specific RNA interference. Our phylogenetic analysis indicated that CpBV-E94ks were clustered with other E94ks originated from different PDVs and shared high similarity with betabaculoviral p94s. These 4 CpBV genes were expressed during most developmental stages of the larvae of Plutella xylostella parasitized by C. plutellae. Expression of these 4 E94ks was mainly detected in hemocytes and fat body. Subsequent functional analysis by in vivo transient expression showed that all 4 viral genes significantly inhibited both host immune and developmental processes. These results suggest that CpBV-E94ks share an origin with betabaculoviral p94s and play parasitic roles in suppressing host immune and developmental processes.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kim Y
Department of Bioresource Sciences, Andong National University, Andong, 760-749, Korea.
Hepat R
Department of Bioresource Sciences, Andong National University, Andong, 760-749, Korea.

MeSH

AnimalsBiological EvolutionFemaleGenes, ViralImmunity, InnateLarvaMolecular Sequence DataMothsPhylogenyPolydnaviridaeRNA InterferenceWasps

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25973570

Citation

Kim, Yonggyun, and Rahul Hepat. "Baculoviral P94 Homologs Encoded in Cotesia Plutellae Bracovirus Suppress Both Immunity and Development of the Diamondback Moth, Plutellae Xylostella." Insect Science, vol. 23, no. 2, 2016, pp. 235-44.
Kim Y, Hepat R. Baculoviral p94 homologs encoded in Cotesia plutellae bracovirus suppress both immunity and development of the diamondback moth, Plutellae xylostella. Insect Sci. 2016;23(2):235-44.
Kim, Y., & Hepat, R. (2016). Baculoviral p94 homologs encoded in Cotesia plutellae bracovirus suppress both immunity and development of the diamondback moth, Plutellae xylostella. Insect Science, 23(2), 235-44. https://doi.org/10.1111/1744-7917.12237
Kim Y, Hepat R. Baculoviral P94 Homologs Encoded in Cotesia Plutellae Bracovirus Suppress Both Immunity and Development of the Diamondback Moth, Plutellae Xylostella. Insect Sci. 2016;23(2):235-44. PubMed PMID: 25973570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Baculoviral p94 homologs encoded in Cotesia plutellae bracovirus suppress both immunity and development of the diamondback moth, Plutellae xylostella. AU - Kim,Yonggyun, AU - Hepat,Rahul, Y1 - 2015/07/15/ PY - 2015/04/22/accepted PY - 2015/5/15/entrez PY - 2015/5/15/pubmed PY - 2016/12/15/medline KW - Cotesia plutellae KW - CpBV-E94k KW - Plutella xylostella KW - baculovirus KW - polydnavirus SP - 235 EP - 44 JF - Insect science JO - Insect Sci VL - 23 IS - 2 N2 - Polydnaviruses (PDVs) are a group of insect DNA viruses, which exhibit a mutual symbiotic relationship with their specific host wasps. Moreover, most encapsidated genes identified so far in PDVs share homologies with insect-originated genes, but not with virus-originated genes. In the meantime, PDVs associated with 2 wasp genera Cotesia and Glytapanteles encode some genes presumably originated from other viruses. Cotesia plutellae bracovirus (CpBV) encodes 4 genes homologous to baculoviral p94: CpBV-E94k1, CpBV-E94k2, CpBV-E94k3, and CpBV-E94k4. This study was conducted to predict the origin of CpBV-E94ks by comparing their sequences with those of baculoviral orthologs and to determine the physiological functions by their transient expressions in nonparasitized larvae and subsequent specific RNA interference. Our phylogenetic analysis indicated that CpBV-E94ks were clustered with other E94ks originated from different PDVs and shared high similarity with betabaculoviral p94s. These 4 CpBV genes were expressed during most developmental stages of the larvae of Plutella xylostella parasitized by C. plutellae. Expression of these 4 E94ks was mainly detected in hemocytes and fat body. Subsequent functional analysis by in vivo transient expression showed that all 4 viral genes significantly inhibited both host immune and developmental processes. These results suggest that CpBV-E94ks share an origin with betabaculoviral p94s and play parasitic roles in suppressing host immune and developmental processes. SN - 1744-7917 UR - https://www.unboundmedicine.com/medline/citation/25973570/Baculoviral_p94_homologs_encoded_in_Cotesia_plutellae_bracovirus_suppress_both_immunity_and_development_of_the_diamondback_moth_Plutellae_xylostella_ DB - PRIME DP - Unbound Medicine ER -