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Omega 3 (n-3) fatty acids down-regulate nuclear factor-kappa B (NF-κB) gene and blood cell adhesion molecule expression in patients with homozygous sickle cell disease.
Blood Cells Mol Dis. 2015 Jun; 55(1):48-55.BC

Abstract

Chronic inflammation and reduced blood levels of omega-3 fatty acids (n-3) are known characteristics of sickle cell disease (SCD).The anti-inflammatory properties of n-3 fatty acids are well recognized. Omega-3 treated (n = 24), hydroxyurea (HU) treated (n = 18), and n-3 untreated (n=21) homozygous SCD patients (HbSS) and healthy (HbAA) controls (n = 25) matched for age (5-16 years), gender and socioeconomic status were studied. According to age (5-10) or (11-16) years, two or three capsules containing 277.8 mg docosahexaenoic (DHA) and 39.0mg eicosapentaenoic (EPA) or high oleic acid placebo (41%) were assigned to n-3 treated and n-3 untreated groups, respectively. Hydroxyurea treated group was on dosage more than 20 mg/kg/day. The effect of supplementation on systemic and blood cell markers of inflammation was investigated. The n-3 treated group had higher levels of DHA and EPA (p < 0.001) and lower white blood cell count and monocyte integrin (p < 0.05) compared with the n-3 untreated. No difference was detected between the two groups regarding C-reactive protein, granulocytes integrin and selectin, plasma tumour necrosis factor-α and interleukin-10. The n-3 treated group had lowered nuclear factor-kappa B (NF-κB) gene expression compared to n-3 untreated and HU treated groups (p < 0.05). This study provides evidence that supplementation with n-3 fatty acids may ameliorate inflammation and blood cell adhesion in patients with SCD.

Authors+Show Affiliations

Lipidomics and Nutrition Research Center, Faculty of Life Sciences and Computing, London Metropolitan University, London, UK; Department of Medical Biochemistry, Faculty of Medicine, University of Khartoum, Sudan; Harvard School of Public Health, Harvard University, USA. Electronic address: ahmed.malik@meduofk.net.School of Pharmacy and Biomedical Sciences, Faculty of Science, University of Portsmouth, UK.Department of Medical Biochemistry, Faculty of Medicine, University of Khartoum, Sudan.Lipidomics and Nutrition Research Center, Faculty of Life Sciences and Computing, London Metropolitan University, London, UK.School of Pharmacy and Biomedical Sciences, Faculty of Science, University of Portsmouth, UK.School of Pharmacy and Biomedical Sciences, Faculty of Science, University of Portsmouth, UK.Department of Medical Biochemistry, Faculty of Medicine, University of Khartoum, Sudan.Lipidomics and Nutrition Research Center, Faculty of Life Sciences and Computing, London Metropolitan University, London, UK.

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25976467

Citation

Daak, Ahmed A., et al. "Omega 3 (n-3) Fatty Acids Down-regulate Nuclear Factor-kappa B (NF-κB) Gene and Blood Cell Adhesion Molecule Expression in Patients With Homozygous Sickle Cell Disease." Blood Cells, Molecules & Diseases, vol. 55, no. 1, 2015, pp. 48-55.
Daak AA, Elderdery AY, Elbashir LM, et al. Omega 3 (n-3) fatty acids down-regulate nuclear factor-kappa B (NF-κB) gene and blood cell adhesion molecule expression in patients with homozygous sickle cell disease. Blood Cells Mol Dis. 2015;55(1):48-55.
Daak, A. A., Elderdery, A. Y., Elbashir, L. M., Mariniello, K., Mills, J., Scarlett, G., Elbashir, M. I., & Ghebremeskel, K. (2015). Omega 3 (n-3) fatty acids down-regulate nuclear factor-kappa B (NF-κB) gene and blood cell adhesion molecule expression in patients with homozygous sickle cell disease. Blood Cells, Molecules & Diseases, 55(1), 48-55. https://doi.org/10.1016/j.bcmd.2015.03.014
Daak AA, et al. Omega 3 (n-3) Fatty Acids Down-regulate Nuclear Factor-kappa B (NF-κB) Gene and Blood Cell Adhesion Molecule Expression in Patients With Homozygous Sickle Cell Disease. Blood Cells Mol Dis. 2015;55(1):48-55. PubMed PMID: 25976467.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Omega 3 (n-3) fatty acids down-regulate nuclear factor-kappa B (NF-κB) gene and blood cell adhesion molecule expression in patients with homozygous sickle cell disease. AU - Daak,Ahmed A, AU - Elderdery,Abozer Y, AU - Elbashir,Leana M, AU - Mariniello,Katia, AU - Mills,Jeremy, AU - Scarlett,Garry, AU - Elbashir,Mustafa I, AU - Ghebremeskel,Kebreab, Y1 - 2015/03/31/ PY - 2015/01/19/received PY - 2015/03/29/revised PY - 2015/03/29/accepted PY - 2015/5/16/entrez PY - 2015/5/16/pubmed PY - 2016/2/4/medline KW - Blood cell adhesion KW - Inflammation KW - Nuclear factor-kappa B (NF-κB) KW - Omega-3 and omega-6 fatty acids KW - Sickle cell disease SP - 48 EP - 55 JF - Blood cells, molecules & diseases JO - Blood Cells Mol Dis VL - 55 IS - 1 N2 - Chronic inflammation and reduced blood levels of omega-3 fatty acids (n-3) are known characteristics of sickle cell disease (SCD).The anti-inflammatory properties of n-3 fatty acids are well recognized. Omega-3 treated (n = 24), hydroxyurea (HU) treated (n = 18), and n-3 untreated (n=21) homozygous SCD patients (HbSS) and healthy (HbAA) controls (n = 25) matched for age (5-16 years), gender and socioeconomic status were studied. According to age (5-10) or (11-16) years, two or three capsules containing 277.8 mg docosahexaenoic (DHA) and 39.0mg eicosapentaenoic (EPA) or high oleic acid placebo (41%) were assigned to n-3 treated and n-3 untreated groups, respectively. Hydroxyurea treated group was on dosage more than 20 mg/kg/day. The effect of supplementation on systemic and blood cell markers of inflammation was investigated. The n-3 treated group had higher levels of DHA and EPA (p < 0.001) and lower white blood cell count and monocyte integrin (p < 0.05) compared with the n-3 untreated. No difference was detected between the two groups regarding C-reactive protein, granulocytes integrin and selectin, plasma tumour necrosis factor-α and interleukin-10. The n-3 treated group had lowered nuclear factor-kappa B (NF-κB) gene expression compared to n-3 untreated and HU treated groups (p < 0.05). This study provides evidence that supplementation with n-3 fatty acids may ameliorate inflammation and blood cell adhesion in patients with SCD. SN - 1096-0961 UR - https://www.unboundmedicine.com/medline/citation/25976467/Omega_3__n_3__fatty_acids_down_regulate_nuclear_factor_kappa_B__NF_κB__gene_and_blood_cell_adhesion_molecule_expression_in_patients_with_homozygous_sickle_cell_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1079-9796(15)00074-1 DB - PRIME DP - Unbound Medicine ER -