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Silver nanoparticles affect the neural development of zebrafish embryos.
J Appl Toxicol. 2015 Dec; 35(12):1481-92.JA

Abstract

Silver nanoparticles (AgNPs) have been widely used in commercial products. This study aims to understand the impact of AgNPs on the early developmental stages in zebrafish (Danio rerio) embryos. Embryos were exposed to two sizes of AgNPs at three dose levels, as well to free Ag(+) ions, for a range of 4-96 h post-fertilization (hpf). The acute exposure study showed that exposure to AgNPs affected the neurological development, and the exposed embryos exhibited anomalies such as small head with hypoplastic hindbrain, small eye and cardiac defects. At the molecular level, AgNPs altered the expression profiles of neural development-related genes (gfap, huC and ngn1), metal-sensitive metallothioneins and ABCC genes in exposed embryos. The expression of AhR2 and Cyp1A, which are usually considered to mediate polycyclic aromatic hydrocarbon toxicity, were also significantly changed. A size-dependent uptake of AgNPs was observed, whereby 4 nm AgNPs were more efficiently taken up compared with the 10 nm-sized particles. Importantly, the head area accumulated AgNPs more efficiently than the trunk area of exposed zebrafish embryos. No free Ag(+) ions, which can be potentially released from the AgNP solutions, were detected. This study suggests that AgNPs could affect the neural development of zebrafish embryos, and the toxicity of AgNPs may be partially attributed to the comparatively higher uptake in the head area. These results indicate the potential neurotoxicity of AgNPs and could be extended to other aquatic organisms.

Authors+Show Affiliations

State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, 200062, China. City University of Hong Kong Shenzhen Research Institute, Shenzhen, 518057, China.School of Biological, Biomedical & Environmental Sciences, University of Hull, Cottingham Road, Hull, HU6 7RX, UK.State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, 200062, China. City University of Hong Kong Shenzhen Research Institute, Shenzhen, 518057, China. Environmental Science Programs, School of Science, Hong Kong University of Science and Technology, Clear Water bay, Kowloon, Hong Kong, China.State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, 200062, China.State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, 200062, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25976698

Citation

Xin, Qi, et al. "Silver Nanoparticles Affect the Neural Development of Zebrafish Embryos." Journal of Applied Toxicology : JAT, vol. 35, no. 12, 2015, pp. 1481-92.
Xin Q, Rotchell JM, Cheng J, et al. Silver nanoparticles affect the neural development of zebrafish embryos. J Appl Toxicol. 2015;35(12):1481-92.
Xin, Q., Rotchell, J. M., Cheng, J., Yi, J., & Zhang, Q. (2015). Silver nanoparticles affect the neural development of zebrafish embryos. Journal of Applied Toxicology : JAT, 35(12), 1481-92. https://doi.org/10.1002/jat.3164
Xin Q, et al. Silver Nanoparticles Affect the Neural Development of Zebrafish Embryos. J Appl Toxicol. 2015;35(12):1481-92. PubMed PMID: 25976698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver nanoparticles affect the neural development of zebrafish embryos. AU - Xin,Qi, AU - Rotchell,Jeanette M, AU - Cheng,Jinping, AU - Yi,Jun, AU - Zhang,Qiang, Y1 - 2015/05/15/ PY - 2015/01/02/received PY - 2015/02/28/revised PY - 2015/03/18/accepted PY - 2015/5/16/entrez PY - 2015/5/16/pubmed PY - 2016/8/10/medline KW - cardiotoxicity KW - developmental toxicity KW - gene expression KW - nanotoxicity KW - neurotoxicity KW - silver nanoparticles KW - zebrafish embryos SP - 1481 EP - 92 JF - Journal of applied toxicology : JAT JO - J Appl Toxicol VL - 35 IS - 12 N2 - Silver nanoparticles (AgNPs) have been widely used in commercial products. This study aims to understand the impact of AgNPs on the early developmental stages in zebrafish (Danio rerio) embryos. Embryos were exposed to two sizes of AgNPs at three dose levels, as well to free Ag(+) ions, for a range of 4-96 h post-fertilization (hpf). The acute exposure study showed that exposure to AgNPs affected the neurological development, and the exposed embryos exhibited anomalies such as small head with hypoplastic hindbrain, small eye and cardiac defects. At the molecular level, AgNPs altered the expression profiles of neural development-related genes (gfap, huC and ngn1), metal-sensitive metallothioneins and ABCC genes in exposed embryos. The expression of AhR2 and Cyp1A, which are usually considered to mediate polycyclic aromatic hydrocarbon toxicity, were also significantly changed. A size-dependent uptake of AgNPs was observed, whereby 4 nm AgNPs were more efficiently taken up compared with the 10 nm-sized particles. Importantly, the head area accumulated AgNPs more efficiently than the trunk area of exposed zebrafish embryos. No free Ag(+) ions, which can be potentially released from the AgNP solutions, were detected. This study suggests that AgNPs could affect the neural development of zebrafish embryos, and the toxicity of AgNPs may be partially attributed to the comparatively higher uptake in the head area. These results indicate the potential neurotoxicity of AgNPs and could be extended to other aquatic organisms. SN - 1099-1263 UR - https://www.unboundmedicine.com/medline/citation/25976698/Silver_nanoparticles_affect_the_neural_development_of_zebrafish_embryos_ L2 - https://doi.org/10.1002/jat.3164 DB - PRIME DP - Unbound Medicine ER -