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Molecular characterization of Staphylococcus aureus isolated from humans related to a livestock farm in Spain, with detection of MRSA-CC130 carrying mecC gene: A zoonotic case?
Enferm Infecc Microbiol Clin. 2016 May; 34(5):280-5.EI

Abstract

OBJECTIVES

To conduct a study of Staphylococcus aureus carriage in members of a livestock-farmer's family with different degrees of animal contact, and to characterize the recovered isolates.

METHODS

Nasal samples from 11 members of the family were taken in three sampling periods (every six months) (n=31), and 9 skin samples from superficial lesions were also obtained in 5 of them. Samples were analyzed for S. aureus susceptible (MSSA) and resistant to methicillin (MRSA). S. aureus isolates were tested for antibiotic-resistance phenotype and genotype and for the detection of virulence and IEC-system genes. Molecular typing of isolates was also performed (spa- and multilocus-sequence typing).

RESULTS

Eighteen S. aureus isolates were recovered (1 MRSA and 17 MSSA) in the 40 samples analyzed. S. aureus was detected in nasal and skin samples of 7/11 and 4/5 of tested humans, respectively. The MRSA strain was detected in the skin lesion of a farmer with high animal contact, and carried the mecC gene, and was typed as ST130-CC130-t843. The 17 MSSA isolates were ascribed to 9 different spa-types and sequence types included in the clonal complexes CC22, CC30, CC45, CC121, and in the livestock-associated lineages CC9 and CC133. Six strains harbored eta or tsst-1 genes. Three of 18 strains lacked the immune-evasion-cluster (IEC) genes (MRSA-ST130, MSSA-ST1333, and MSSA-ST133), and the remaining isolates were ascribed to IEC type-A or -B.

CONCLUSIONS

Animal-associated S. aureus lineages were detected in samples of the farmer's family, highlighting the detection of MSSA-CC133 and mecC-MRSA-ST130.

Authors+Show Affiliations

Área Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain.Área Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain.Unidad de Microbiología. Hospital Royo Villanova, Zaragoza, Spain.Área Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain.Área Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain.Área Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain. Electronic address: carmen.torres@unirioja.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25976751

Citation

Benito, Daniel, et al. "Molecular Characterization of Staphylococcus Aureus Isolated From Humans Related to a Livestock Farm in Spain, With Detection of MRSA-CC130 Carrying mecC Gene: a Zoonotic Case?" Enfermedades Infecciosas Y Microbiologia Clinica, vol. 34, no. 5, 2016, pp. 280-5.
Benito D, Gómez P, Aspiroz C, et al. Molecular characterization of Staphylococcus aureus isolated from humans related to a livestock farm in Spain, with detection of MRSA-CC130 carrying mecC gene: A zoonotic case? Enferm Infecc Microbiol Clin. 2016;34(5):280-5.
Benito, D., Gómez, P., Aspiroz, C., Zarazaga, M., Lozano, C., & Torres, C. (2016). Molecular characterization of Staphylococcus aureus isolated from humans related to a livestock farm in Spain, with detection of MRSA-CC130 carrying mecC gene: A zoonotic case? Enfermedades Infecciosas Y Microbiologia Clinica, 34(5), 280-5. https://doi.org/10.1016/j.eimc.2015.03.008
Benito D, et al. Molecular Characterization of Staphylococcus Aureus Isolated From Humans Related to a Livestock Farm in Spain, With Detection of MRSA-CC130 Carrying mecC Gene: a Zoonotic Case. Enferm Infecc Microbiol Clin. 2016;34(5):280-5. PubMed PMID: 25976751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of Staphylococcus aureus isolated from humans related to a livestock farm in Spain, with detection of MRSA-CC130 carrying mecC gene: A zoonotic case? AU - Benito,Daniel, AU - Gómez,Paula, AU - Aspiroz,Carmen, AU - Zarazaga,Myriam, AU - Lozano,Carmen, AU - Torres,Carmen, Y1 - 2015/05/11/ PY - 2015/02/09/received PY - 2015/03/16/revised PY - 2015/03/17/accepted PY - 2015/5/16/entrez PY - 2015/5/16/pubmed PY - 2017/10/24/medline KW - CC130 KW - CC133 KW - Farmers KW - Granjeros KW - S. aureus KW - mecC SP - 280 EP - 5 JF - Enfermedades infecciosas y microbiologia clinica JO - Enferm Infecc Microbiol Clin VL - 34 IS - 5 N2 - OBJECTIVES: To conduct a study of Staphylococcus aureus carriage in members of a livestock-farmer's family with different degrees of animal contact, and to characterize the recovered isolates. METHODS: Nasal samples from 11 members of the family were taken in three sampling periods (every six months) (n=31), and 9 skin samples from superficial lesions were also obtained in 5 of them. Samples were analyzed for S. aureus susceptible (MSSA) and resistant to methicillin (MRSA). S. aureus isolates were tested for antibiotic-resistance phenotype and genotype and for the detection of virulence and IEC-system genes. Molecular typing of isolates was also performed (spa- and multilocus-sequence typing). RESULTS: Eighteen S. aureus isolates were recovered (1 MRSA and 17 MSSA) in the 40 samples analyzed. S. aureus was detected in nasal and skin samples of 7/11 and 4/5 of tested humans, respectively. The MRSA strain was detected in the skin lesion of a farmer with high animal contact, and carried the mecC gene, and was typed as ST130-CC130-t843. The 17 MSSA isolates were ascribed to 9 different spa-types and sequence types included in the clonal complexes CC22, CC30, CC45, CC121, and in the livestock-associated lineages CC9 and CC133. Six strains harbored eta or tsst-1 genes. Three of 18 strains lacked the immune-evasion-cluster (IEC) genes (MRSA-ST130, MSSA-ST1333, and MSSA-ST133), and the remaining isolates were ascribed to IEC type-A or -B. CONCLUSIONS: Animal-associated S. aureus lineages were detected in samples of the farmer's family, highlighting the detection of MSSA-CC133 and mecC-MRSA-ST130. SN - 1578-1852 UR - https://www.unboundmedicine.com/medline/citation/25976751/Molecular_characterization_of_Staphylococcus_aureus_isolated_from_humans_related_to_a_livestock_farm_in_Spain_with_detection_of_MRSA_CC130_carrying_mecC_gene:_A_zoonotic_case L2 - https://linkinghub.elsevier.com/retrieve/pii/S0213-005X(15)00118-4 DB - PRIME DP - Unbound Medicine ER -