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Dissection of the style's response to pollination using transcriptome profiling in self-compatible (Solanum pimpinellifolium) and self-incompatible (Solanum chilense) tomato species.
BMC Plant Biol 2015; 15:119BP

Abstract

BACKGROUND

Tomato (Solanum lycopersicum) self-compatibility (SC) is defined as self-pollen tubes that can penetrate their own stigma, elongate in the style and fertilize their own ovules. Self-incompatibility (SI) is defined as self-pollen tubes that are prevented from developing in the style. To determine the influence of gene expression on style self-pollination, a transcriptome-wide comparative analysis of SC and SI tomato unpollinated/pollinated styles was performed using RNA-sequencing (RNA-seq) data.

RESULTS

Transcriptome profiles of 24-h unpollination (UP) and self-pollination (P) styles from SC and SI tomato species were generated using high-throughput next generation sequencing. From the comparison of SC self-pollinated and unpollinated styles, 1341 differentially expressed genes (DEGs) were identified, of which 753 were downregulated and 588 were upregulated. From the comparison of SI self-pollinated and unpollinated styles, 804 DEGs were identified, of which 215 were downregulated and 589 were upregulated. Nine gene ontology (GO) terms were enriched significantly in SC and 78 GO terms were enriched significantly in SI. A total of 105 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were identified in SC and 80 enriched KEGG pathways were identified in SI, among which "Cysteine and methionine metabolism pathway" and "Plant hormone signal transduction pathway" were significantly enriched in SI.

CONCLUSIONS

This study is the first global transcriptome-wide comparative analysis of SC and SI tomato unpollinated/pollinated styles. Advanced bioinformatic analysis of DEGs uncovered the pathways of "Cysteine and methionine metabolism" and "Plant hormone signal transduction", which are likely to play important roles in the control of pollen tubes growth in SI species.

Authors+Show Affiliations

Joint Tomato Research Institute, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. shuainanhai81517@sohu.com. Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. shuainanhai81517@sohu.com.Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. zhangld@sjtu.edu.cn.Joint Tomato Research Institute, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. lxzhao@sjtu.edu.cn. Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. lxzhao@sjtu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25976872

Citation

Zhao, Panfeng, et al. "Dissection of the Style's Response to Pollination Using Transcriptome Profiling in Self-compatible (Solanum Pimpinellifolium) and Self-incompatible (Solanum Chilense) Tomato Species." BMC Plant Biology, vol. 15, 2015, p. 119.
Zhao P, Zhang L, Zhao L. Dissection of the style's response to pollination using transcriptome profiling in self-compatible (Solanum pimpinellifolium) and self-incompatible (Solanum chilense) tomato species. BMC Plant Biol. 2015;15:119.
Zhao, P., Zhang, L., & Zhao, L. (2015). Dissection of the style's response to pollination using transcriptome profiling in self-compatible (Solanum pimpinellifolium) and self-incompatible (Solanum chilense) tomato species. BMC Plant Biology, 15, p. 119. doi:10.1186/s12870-015-0492-7.
Zhao P, Zhang L, Zhao L. Dissection of the Style's Response to Pollination Using Transcriptome Profiling in Self-compatible (Solanum Pimpinellifolium) and Self-incompatible (Solanum Chilense) Tomato Species. BMC Plant Biol. 2015 May 15;15:119. PubMed PMID: 25976872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dissection of the style's response to pollination using transcriptome profiling in self-compatible (Solanum pimpinellifolium) and self-incompatible (Solanum chilense) tomato species. AU - Zhao,Panfeng, AU - Zhang,Lida, AU - Zhao,Lingxia, Y1 - 2015/05/15/ PY - 2015/02/07/received PY - 2015/04/10/accepted PY - 2015/5/16/entrez PY - 2015/5/16/pubmed PY - 2016/1/27/medline SP - 119 EP - 119 JF - BMC plant biology JO - BMC Plant Biol. VL - 15 N2 - BACKGROUND: Tomato (Solanum lycopersicum) self-compatibility (SC) is defined as self-pollen tubes that can penetrate their own stigma, elongate in the style and fertilize their own ovules. Self-incompatibility (SI) is defined as self-pollen tubes that are prevented from developing in the style. To determine the influence of gene expression on style self-pollination, a transcriptome-wide comparative analysis of SC and SI tomato unpollinated/pollinated styles was performed using RNA-sequencing (RNA-seq) data. RESULTS: Transcriptome profiles of 24-h unpollination (UP) and self-pollination (P) styles from SC and SI tomato species were generated using high-throughput next generation sequencing. From the comparison of SC self-pollinated and unpollinated styles, 1341 differentially expressed genes (DEGs) were identified, of which 753 were downregulated and 588 were upregulated. From the comparison of SI self-pollinated and unpollinated styles, 804 DEGs were identified, of which 215 were downregulated and 589 were upregulated. Nine gene ontology (GO) terms were enriched significantly in SC and 78 GO terms were enriched significantly in SI. A total of 105 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were identified in SC and 80 enriched KEGG pathways were identified in SI, among which "Cysteine and methionine metabolism pathway" and "Plant hormone signal transduction pathway" were significantly enriched in SI. CONCLUSIONS: This study is the first global transcriptome-wide comparative analysis of SC and SI tomato unpollinated/pollinated styles. Advanced bioinformatic analysis of DEGs uncovered the pathways of "Cysteine and methionine metabolism" and "Plant hormone signal transduction", which are likely to play important roles in the control of pollen tubes growth in SI species. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/25976872/Dissection_of_the_style's_response_to_pollination_using_transcriptome_profiling_in_self_compatible__Solanum_pimpinellifolium__and_self_incompatible__Solanum_chilense__tomato_species_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-015-0492-7 DB - PRIME DP - Unbound Medicine ER -