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Fast determination of biogenic amines in beverages by a core-shell particle column.
Food Chem. 2015 Nov 15; 187:555-62.FC

Abstract

A fast and reliable HPLC method for the determination of 11 biogenic amines in beverages has been performed. After pre-column derivatization with dansyl-chloride a Kinetex C18 core-shell particle column (100 mm × 4.6 mm, 2.6 μm particle size) has been employed and the biogenic amines were identified and quantified in a total run time of 13 min with ultraviolet (UV) or fluorescence detection (FLD). Chromatographic conditions such as column temperature (kept at 50 °C), gradient elution and flow rate have been optimized and the method has been tested on red wine and fruit nectar. The proposed method is enhanced in terms of reduced analysis time and eluent consumption with respect of classical HPLC method as to be comparable to UHPLC methods. Green and cost-effective, this method can be used as a quality-control tool for routine quantitative analysis of biogenic amines in beverages for the average laboratory.

Authors+Show Affiliations

Laboratory of Commodity Science, Department of Management, Sapienza University of Rome, via del Castro Laurenziano 9, 00161 Rome, Italy. Electronic address: raffaella.preti@uniroma1.it.Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00161 Rome, Italy.Laboratory of Commodity Science, Department of Management, Sapienza University of Rome, via del Castro Laurenziano 9, 00161 Rome, Italy.Laboratory of Commodity Science, Department of Management, Sapienza University of Rome, via del Castro Laurenziano 9, 00161 Rome, Italy.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

25977063

Citation

Preti, Raffaella, et al. "Fast Determination of Biogenic Amines in Beverages By a Core-shell Particle Column." Food Chemistry, vol. 187, 2015, pp. 555-62.
Preti R, Antonelli ML, Bernacchia R, et al. Fast determination of biogenic amines in beverages by a core-shell particle column. Food Chem. 2015;187:555-62.
Preti, R., Antonelli, M. L., Bernacchia, R., & Vinci, G. (2015). Fast determination of biogenic amines in beverages by a core-shell particle column. Food Chemistry, 187, 555-62. https://doi.org/10.1016/j.foodchem.2015.04.075
Preti R, et al. Fast Determination of Biogenic Amines in Beverages By a Core-shell Particle Column. Food Chem. 2015 Nov 15;187:555-62. PubMed PMID: 25977063.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast determination of biogenic amines in beverages by a core-shell particle column. AU - Preti,Raffaella, AU - Antonelli,Marta Letizia, AU - Bernacchia,Roberta, AU - Vinci,Giuliana, Y1 - 2015/04/24/ PY - 2014/07/01/received PY - 2014/12/22/revised PY - 2015/04/05/accepted PY - 2015/5/16/entrez PY - 2015/5/16/pubmed PY - 2016/1/12/medline KW - Agmatine (PubChem CID: 199) KW - Biogenic amines KW - Cadaverine (PubChem CID: 273) KW - Core–shell particle column KW - Dansyl chloride KW - Ethylamine (PubChem CID: 6341) KW - Fruit nectar KW - HPLC KW - Histamine (PubChem CID: 774) KW - Methylamine (PubChem CID: 6329) KW - Putrescine (PubChem CID: 1045) KW - Serotonin (PubChem CID: 5202) KW - Spermidine (PubChem CID: 1102) KW - Spermine (PubChem CID: 1103) KW - Tyramine (PubChem CID: 560) KW - Wine SP - 555 EP - 62 JF - Food chemistry JO - Food Chem VL - 187 N2 - A fast and reliable HPLC method for the determination of 11 biogenic amines in beverages has been performed. After pre-column derivatization with dansyl-chloride a Kinetex C18 core-shell particle column (100 mm × 4.6 mm, 2.6 μm particle size) has been employed and the biogenic amines were identified and quantified in a total run time of 13 min with ultraviolet (UV) or fluorescence detection (FLD). Chromatographic conditions such as column temperature (kept at 50 °C), gradient elution and flow rate have been optimized and the method has been tested on red wine and fruit nectar. The proposed method is enhanced in terms of reduced analysis time and eluent consumption with respect of classical HPLC method as to be comparable to UHPLC methods. Green and cost-effective, this method can be used as a quality-control tool for routine quantitative analysis of biogenic amines in beverages for the average laboratory. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/25977063/Fast_determination_of_biogenic_amines_in_beverages_by_a_core_shell_particle_column_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(15)00621-4 DB - PRIME DP - Unbound Medicine ER -