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A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles.
Ecotoxicol Environ Saf. 2015 Sep; 119:66-73.EE

Abstract

We examined the short-term toxicity of AgNPs and AgNO3 to Daphnia magna at sublethal levels using (1)H NMR-based metabolomics. Two sizes of polyvinylpyrrolidone-coated AgNPs (10 and 40nm) were synthesized and characterized and their Ag(+) release was studied using centrifugal ultrafiltration and inductively coupled plasma mass spectrometry. Multivariate statistical analysis of the (1)H NMR spectra showed significant changes in the D. magna metabolic profiles following 48h exposure to both AgNP particle sizes and Ag(+) exposure. Most of the metabolic biomarkers for AgNP exposure, including 3-hydroxybutyrate, arginine, lysine and phosphocholine, were identical to those of the Ag(+)-exposed groups, suggesting that the dominant effects of both AgNPs were due to released Ag(+). The observed metabolic changes implied that the released Ag(+) induced disturbance in energy metabolism and oxidative stress, a proposed mechanism of AgNP toxicity. Elevated levels of lactate in all AgNP-treated but not in Ag(+)-treated groups provided evidence for Ag-NP enhanced anaerobic metabolism. These findings show that (1)H NMR-based metabolomics provides a sensitive measure of D. magna response to AgNPs and that further targeted assays are needed to elucidate mechanisms of action of nanoparticle-induced toxicity.

Authors+Show Affiliations

Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, no.17, Chunhui Road, Yantai, Shandong 264003, PR China.Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, no.17, Chunhui Road, Yantai, Shandong 264003, PR China. Electronic address: hfwu@yic.ac.cn.Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, no.17, Chunhui Road, Yantai, Shandong 264003, PR China.Department of Ecological Science, Faculty of Earth and Life Sciences, VU University, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.Department of Civil and Environmental Engineering, University of Delaware, Newark, DE 19716, USA.National Institute of Public Health and the Environment, Center for Safety of Substances and Products, P.O. Box 1, 3720 BA Bilthoven, The Netherlands; Institute of Environmental Sciences (CML), Leiden University, Leiden, The Netherlands.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

25978415

Citation

Li, LianZhen, et al. "A Metabolomic Study On the Responses of Daphnia Magna Exposed to Silver Nitrate and Coated Silver Nanoparticles." Ecotoxicology and Environmental Safety, vol. 119, 2015, pp. 66-73.
Li L, Wu H, Ji C, et al. A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles. Ecotoxicol Environ Saf. 2015;119:66-73.
Li, L., Wu, H., Ji, C., van Gestel, C. A., Allen, H. E., & Peijnenburg, W. J. (2015). A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles. Ecotoxicology and Environmental Safety, 119, 66-73. https://doi.org/10.1016/j.ecoenv.2015.05.005
Li L, et al. A Metabolomic Study On the Responses of Daphnia Magna Exposed to Silver Nitrate and Coated Silver Nanoparticles. Ecotoxicol Environ Saf. 2015;119:66-73. PubMed PMID: 25978415.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles. AU - Li,LianZhen, AU - Wu,Huifeng, AU - Ji,Chenglong, AU - van Gestel,Cornelis A M, AU - Allen,Herbert E, AU - Peijnenburg,Willie J G M, Y1 - 2015/05/14/ PY - 2014/12/30/received PY - 2015/05/05/revised PY - 2015/05/06/accepted PY - 2015/5/16/entrez PY - 2015/5/16/pubmed PY - 2016/2/5/medline KW - Daphnia magna KW - Metabolomics KW - Silver ion KW - Silver nanoparticle KW - Sublethal toxicity SP - 66 EP - 73 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 119 N2 - We examined the short-term toxicity of AgNPs and AgNO3 to Daphnia magna at sublethal levels using (1)H NMR-based metabolomics. Two sizes of polyvinylpyrrolidone-coated AgNPs (10 and 40nm) were synthesized and characterized and their Ag(+) release was studied using centrifugal ultrafiltration and inductively coupled plasma mass spectrometry. Multivariate statistical analysis of the (1)H NMR spectra showed significant changes in the D. magna metabolic profiles following 48h exposure to both AgNP particle sizes and Ag(+) exposure. Most of the metabolic biomarkers for AgNP exposure, including 3-hydroxybutyrate, arginine, lysine and phosphocholine, were identical to those of the Ag(+)-exposed groups, suggesting that the dominant effects of both AgNPs were due to released Ag(+). The observed metabolic changes implied that the released Ag(+) induced disturbance in energy metabolism and oxidative stress, a proposed mechanism of AgNP toxicity. Elevated levels of lactate in all AgNP-treated but not in Ag(+)-treated groups provided evidence for Ag-NP enhanced anaerobic metabolism. These findings show that (1)H NMR-based metabolomics provides a sensitive measure of D. magna response to AgNPs and that further targeted assays are needed to elucidate mechanisms of action of nanoparticle-induced toxicity. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/25978415/A_metabolomic_study_on_the_responses_of_daphnia_magna_exposed_to_silver_nitrate_and_coated_silver_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(15)00228-6 DB - PRIME DP - Unbound Medicine ER -