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Gli1 promotes transforming growth factor-beta1- and epidermal growth factor-induced epithelial to mesenchymal transition in pancreatic cancer cells.
Surgery. 2015 Jul; 158(1):211-24.S

Abstract

BACKGROUND

The Hedgehog signaling pathway and its key target effector Gli1 are linked closely to the development of the epithelial to mesenchymal transition (EMT) in many cancers. The definite function of Gli1 in regulating the EMT of pancreatic cancer (PC), however, is still unclear.

METHODS

At the cell and tissue levels, we investigated the role of Gli1 in the initiation of EMT in PC with and without external stimulus treatments.

RESULTS

The immunohistochemistry results showed that Gli1 was associated positively with MMP9 but not with E-cad or Vimentin. Gli1 expression was associated positively with tumor T (P = .025) and Union for International Cancer Control stage (P = .032), whereas MMP9 expression was associated positively with lymph node metastasis (P = .017) and Union for International Cancer Control stage (P = .006). Furthermore, patients with Gli1 and MMP9 coexpression had poor overall survival (P = .015). Silencing of Gli1 alone without external stimulus had no effect on EMT but inhibited transforming growth factor-beta1 (TGFβ1)- and epidermal growth factor (EGF)-induced EMT in PANC-1, AsPC-1, and BxPC-3 PC cell lines, along with the inhibition of TGFβ1- and EGF-induced EMT-like cell morphology and invasion, down-regulation of E-cad, and up-regulation of MMP9 and Vimentin in those 3 cell lines, respectively.

CONCLUSION

Gli1 silencing alone has no effect on EMT initiation; however, it exerts a protumor role in the aggressive invasion of PC cells by promoting TGFβ1- and EGF-induced EMT.

Authors+Show Affiliations

Department of General Surgery, Gastrointestinal Surgery, the First Hospital, China Medical University, Shenyang, China; Department of General Surgery, the People's Hospital of Liaoning Province, Shenyang, China.Department of General Surgery, Gastrointestinal Surgery, the First Hospital, China Medical University, Shenyang, China.Department of General Surgery, Gastrointestinal Surgery, the First Hospital, China Medical University, Shenyang, China. Electronic address: cmumingdong@sohu.com.Department of Surgical Oncology, the First Hospital, China Medical University, Shenyang, China.Department of General Surgery, the People's Hospital of Liaoning Province, Shenyang, China.Department of Cell Biology, China Medical University, Shenyang, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25979438

Citation

Liu, Qingfeng, et al. "Gli1 Promotes Transforming Growth Factor-beta1- and Epidermal Growth Factor-induced Epithelial to Mesenchymal Transition in Pancreatic Cancer Cells." Surgery, vol. 158, no. 1, 2015, pp. 211-24.
Liu Q, Sheng W, Dong M, et al. Gli1 promotes transforming growth factor-beta1- and epidermal growth factor-induced epithelial to mesenchymal transition in pancreatic cancer cells. Surgery. 2015;158(1):211-24.
Liu, Q., Sheng, W., Dong, M., Dong, X., Dong, Q., & Li, F. (2015). Gli1 promotes transforming growth factor-beta1- and epidermal growth factor-induced epithelial to mesenchymal transition in pancreatic cancer cells. Surgery, 158(1), 211-24. https://doi.org/10.1016/j.surg.2015.03.016
Liu Q, et al. Gli1 Promotes Transforming Growth Factor-beta1- and Epidermal Growth Factor-induced Epithelial to Mesenchymal Transition in Pancreatic Cancer Cells. Surgery. 2015;158(1):211-24. PubMed PMID: 25979438.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gli1 promotes transforming growth factor-beta1- and epidermal growth factor-induced epithelial to mesenchymal transition in pancreatic cancer cells. AU - Liu,Qingfeng, AU - Sheng,Weiwei, AU - Dong,Ming, AU - Dong,Xiaoshen, AU - Dong,Qi, AU - Li,Feng, PY - 2014/12/30/received PY - 2015/03/21/revised PY - 2015/03/24/accepted PY - 2015/5/17/entrez PY - 2015/5/17/pubmed PY - 2015/8/8/medline SP - 211 EP - 24 JF - Surgery JO - Surgery VL - 158 IS - 1 N2 - BACKGROUND: The Hedgehog signaling pathway and its key target effector Gli1 are linked closely to the development of the epithelial to mesenchymal transition (EMT) in many cancers. The definite function of Gli1 in regulating the EMT of pancreatic cancer (PC), however, is still unclear. METHODS: At the cell and tissue levels, we investigated the role of Gli1 in the initiation of EMT in PC with and without external stimulus treatments. RESULTS: The immunohistochemistry results showed that Gli1 was associated positively with MMP9 but not with E-cad or Vimentin. Gli1 expression was associated positively with tumor T (P = .025) and Union for International Cancer Control stage (P = .032), whereas MMP9 expression was associated positively with lymph node metastasis (P = .017) and Union for International Cancer Control stage (P = .006). Furthermore, patients with Gli1 and MMP9 coexpression had poor overall survival (P = .015). Silencing of Gli1 alone without external stimulus had no effect on EMT but inhibited transforming growth factor-beta1 (TGFβ1)- and epidermal growth factor (EGF)-induced EMT in PANC-1, AsPC-1, and BxPC-3 PC cell lines, along with the inhibition of TGFβ1- and EGF-induced EMT-like cell morphology and invasion, down-regulation of E-cad, and up-regulation of MMP9 and Vimentin in those 3 cell lines, respectively. CONCLUSION: Gli1 silencing alone has no effect on EMT initiation; however, it exerts a protumor role in the aggressive invasion of PC cells by promoting TGFβ1- and EGF-induced EMT. SN - 1532-7361 UR - https://www.unboundmedicine.com/medline/citation/25979438/Gli1_promotes_transforming_growth_factor_beta1__and_epidermal_growth_factor_induced_epithelial_to_mesenchymal_transition_in_pancreatic_cancer_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-6060(15)00208-1 DB - PRIME DP - Unbound Medicine ER -