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Transgenic poplar expressing Arabidopsis YUCCA6 exhibits auxin-overproduction phenotypes and increased tolerance to abiotic stress.
Plant Physiol Biochem. 2015 Sep; 94:19-27.PP

Abstract

YUCCA6, a member of the YUCCA family of flavin monooxygenase-like proteins, is involved in the tryptophan-dependent IAA biosynthesis pathway and responses to environmental cues in Arabidopsis. However, little is known about the role of the YUCCA pathway in auxin biosynthesis in poplar. Here, we generated transgenic poplar (Populus alba × P. glandulosa) expressing the Arabidopsis YUCCA6 gene under the control of the oxidative stress-inducible SWPA2 promoter (referred to as SY plants). Three SY lines (SY7, SY12 and SY20) were selected based on the levels of AtYUCCA6 transcript. SY plants displayed auxin-overproduction morphological phenotypes, such as rapid shoot growth and retarded main root development with increased root hair formation. In addition, SY plants had higher levels of free IAA and early auxin-response gene transcripts. SY plants exhibited tolerance to drought stress, which was associated with reduced levels of reactive oxygen species. Furthermore, SY plants showed delayed hormone- and dark-induced senescence in detached leaves due to higher photosystem II efficiency and less membrane permeability. These results suggest that the conserved IAA biosynthesis pathway mediated by YUCCA family members exists in poplar.

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Authors+Show Affiliations

Ke Q
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, South Korea; Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology, Daejeon 305-350, South Korea.
Wang Z
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Science and Ministry of Water Resources, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Ji CY
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, South Korea; Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology, Daejeon 305-350, South Korea.
Jeong JC
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, South Korea; Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology, Daejeon 305-350, South Korea.
Lee HS
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, South Korea; Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology, Daejeon 305-350, South Korea.
Li H
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Science and Ministry of Water Resources, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Xu B
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Science and Ministry of Water Resources, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Deng X
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Science and Ministry of Water Resources, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Kwak SS
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, South Korea; Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology, Daejeon 305-350, South Korea. Electronic address: sskwak@kribb.re.kr.

MeSH

ArabidopsisArabidopsis ProteinsIndoleacetic AcidsMixed Function OxygenasesPlants, Genetically ModifiedPopulusStress, Physiological

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25980973

Citation

Ke, Qingbo, et al. "Transgenic Poplar Expressing Arabidopsis YUCCA6 Exhibits Auxin-overproduction Phenotypes and Increased Tolerance to Abiotic Stress." Plant Physiology and Biochemistry : PPB, vol. 94, 2015, pp. 19-27.
Ke Q, Wang Z, Ji CY, et al. Transgenic poplar expressing Arabidopsis YUCCA6 exhibits auxin-overproduction phenotypes and increased tolerance to abiotic stress. Plant Physiol Biochem. 2015;94:19-27.
Ke, Q., Wang, Z., Ji, C. Y., Jeong, J. C., Lee, H. S., Li, H., Xu, B., Deng, X., & Kwak, S. S. (2015). Transgenic poplar expressing Arabidopsis YUCCA6 exhibits auxin-overproduction phenotypes and increased tolerance to abiotic stress. Plant Physiology and Biochemistry : PPB, 94, 19-27. https://doi.org/10.1016/j.plaphy.2015.05.003
Ke Q, et al. Transgenic Poplar Expressing Arabidopsis YUCCA6 Exhibits Auxin-overproduction Phenotypes and Increased Tolerance to Abiotic Stress. Plant Physiol Biochem. 2015;94:19-27. PubMed PMID: 25980973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transgenic poplar expressing Arabidopsis YUCCA6 exhibits auxin-overproduction phenotypes and increased tolerance to abiotic stress. AU - Ke,Qingbo, AU - Wang,Zhi, AU - Ji,Chang Yoon, AU - Jeong,Jae Cheol, AU - Lee,Haeng-Soon, AU - Li,Hongbing, AU - Xu,Bingcheng, AU - Deng,Xiping, AU - Kwak,Sang-Soo, Y1 - 2015/05/09/ PY - 2015/04/02/received PY - 2015/05/04/revised PY - 2015/05/08/accepted PY - 2015/5/19/entrez PY - 2015/5/20/pubmed PY - 2016/4/19/medline KW - Abiotic stress KW - AtYUCCA6 KW - Auxin KW - ROS KW - Transgenic poplar SP - 19 EP - 27 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 94 N2 - YUCCA6, a member of the YUCCA family of flavin monooxygenase-like proteins, is involved in the tryptophan-dependent IAA biosynthesis pathway and responses to environmental cues in Arabidopsis. However, little is known about the role of the YUCCA pathway in auxin biosynthesis in poplar. Here, we generated transgenic poplar (Populus alba × P. glandulosa) expressing the Arabidopsis YUCCA6 gene under the control of the oxidative stress-inducible SWPA2 promoter (referred to as SY plants). Three SY lines (SY7, SY12 and SY20) were selected based on the levels of AtYUCCA6 transcript. SY plants displayed auxin-overproduction morphological phenotypes, such as rapid shoot growth and retarded main root development with increased root hair formation. In addition, SY plants had higher levels of free IAA and early auxin-response gene transcripts. SY plants exhibited tolerance to drought stress, which was associated with reduced levels of reactive oxygen species. Furthermore, SY plants showed delayed hormone- and dark-induced senescence in detached leaves due to higher photosystem II efficiency and less membrane permeability. These results suggest that the conserved IAA biosynthesis pathway mediated by YUCCA family members exists in poplar. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/25980973/Transgenic_poplar_expressing_Arabidopsis_YUCCA6_exhibits_auxin_overproduction_phenotypes_and_increased_tolerance_to_abiotic_stress_ DB - PRIME DP - Unbound Medicine ER -