Tags

Type your tag names separated by a space and hit enter

A QTL with major effect on reducing leaf rust severity on the short arm of chromosome 1A of wheat detected across different genetic backgrounds and diverse environments.
Theor Appl Genet. 2015 Aug; 128(8):1579-94.TA

Abstract

KEY MESSAGE

Selection for QLr.cau - 1AS (a major QTL detected in wheat for reducing leaf rust severity) based on the DNA marker gpw2246 was as effective as selection for Lr34 based on cssfr5. Leaf rust is an important disease of wheat worldwide. Utilization of slow-rusting resistance constitutes a strategy to sustainably control this disease. The American wheat cultivar Luke exhibits slow leaf-rusting resistance at the adult plant stage. The objectives of this study were to detect and validate QTL for the resistance in Luke. Three winter wheat populations were used, namely, 149 recombinant inbred lines (RILs) derived from the cross Luke × Aquileja, 307 RILs from Luke × AQ24788-83, and 80 F2:3 families selected from Lingxing66 × KA298. Aquileja and Lingxing66 are highly susceptible to leaf rust. AQ24788-83 shows high (susceptible) infection type but contains the slow-rusting gene Lr34 as diagnosed by the gene-specific marker cssfr5. KA298, an F9 RIL selected from Luke × AQ24788-83, contains Lr34 and QLr.cau-1AS (a major QTL originated from Luke, this study). These wheats were evaluated for leaf rust in 12 field and greenhouse environments involving four locations and five seasons. Genotyping was done using simple sequence repeat (SSR) and diversity arrays technology markers. Of the detected QTLs, QLr.cau-1AS was significant consistently across all the genetic backgrounds, test environments, and likely a wide range of pathogen races. QLr.cau-1AS explained 22.3-55.2% of leaf rust phenotypic variation, being comparable to Lr34 in effect size. A co-dominant SSR marker (gpw2246, http://wheat.pw.usda.gov/GG2/index.shtml) was identified to be tightly linked to QLr.cau-1AS. Selection based on gpw2246 for QLr.cau-1AS was as effective as the selection based on cssfr5 for Lr34. QLr.cau-1AS will be helpful for increasing the genetic diversity of slow leaf-rusting resistance in wheat breeding programs.

Authors+Show Affiliations

Department of Plant Pathology, China Agricultural University, Beijing, 100193, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25982130

Citation

Du, Ziyi, et al. "A QTL With Major Effect On Reducing Leaf Rust Severity On the Short Arm of Chromosome 1A of Wheat Detected Across Different Genetic Backgrounds and Diverse Environments." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 128, no. 8, 2015, pp. 1579-94.
Du Z, Che M, Li G, et al. A QTL with major effect on reducing leaf rust severity on the short arm of chromosome 1A of wheat detected across different genetic backgrounds and diverse environments. Theor Appl Genet. 2015;128(8):1579-94.
Du, Z., Che, M., Li, G., Chen, J., Quan, W., Guo, Y., Wang, Z., Ren, J., Zhang, H., & Zhang, Z. (2015). A QTL with major effect on reducing leaf rust severity on the short arm of chromosome 1A of wheat detected across different genetic backgrounds and diverse environments. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 128(8), 1579-94. https://doi.org/10.1007/s00122-015-2533-x
Du Z, et al. A QTL With Major Effect On Reducing Leaf Rust Severity On the Short Arm of Chromosome 1A of Wheat Detected Across Different Genetic Backgrounds and Diverse Environments. Theor Appl Genet. 2015;128(8):1579-94. PubMed PMID: 25982130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A QTL with major effect on reducing leaf rust severity on the short arm of chromosome 1A of wheat detected across different genetic backgrounds and diverse environments. AU - Du,Ziyi, AU - Che,Mingzhe, AU - Li,Guohui, AU - Chen,Jiang, AU - Quan,Wei, AU - Guo,Yan, AU - Wang,Zhen, AU - Ren,Junda, AU - Zhang,Huyan, AU - Zhang,Zhongjun, Y1 - 2015/05/16/ PY - 2015/01/15/received PY - 2015/05/02/accepted PY - 2015/5/19/entrez PY - 2015/5/20/pubmed PY - 2015/9/19/medline SP - 1579 EP - 94 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 128 IS - 8 N2 - KEY MESSAGE: Selection for QLr.cau - 1AS (a major QTL detected in wheat for reducing leaf rust severity) based on the DNA marker gpw2246 was as effective as selection for Lr34 based on cssfr5. Leaf rust is an important disease of wheat worldwide. Utilization of slow-rusting resistance constitutes a strategy to sustainably control this disease. The American wheat cultivar Luke exhibits slow leaf-rusting resistance at the adult plant stage. The objectives of this study were to detect and validate QTL for the resistance in Luke. Three winter wheat populations were used, namely, 149 recombinant inbred lines (RILs) derived from the cross Luke × Aquileja, 307 RILs from Luke × AQ24788-83, and 80 F2:3 families selected from Lingxing66 × KA298. Aquileja and Lingxing66 are highly susceptible to leaf rust. AQ24788-83 shows high (susceptible) infection type but contains the slow-rusting gene Lr34 as diagnosed by the gene-specific marker cssfr5. KA298, an F9 RIL selected from Luke × AQ24788-83, contains Lr34 and QLr.cau-1AS (a major QTL originated from Luke, this study). These wheats were evaluated for leaf rust in 12 field and greenhouse environments involving four locations and five seasons. Genotyping was done using simple sequence repeat (SSR) and diversity arrays technology markers. Of the detected QTLs, QLr.cau-1AS was significant consistently across all the genetic backgrounds, test environments, and likely a wide range of pathogen races. QLr.cau-1AS explained 22.3-55.2% of leaf rust phenotypic variation, being comparable to Lr34 in effect size. A co-dominant SSR marker (gpw2246, http://wheat.pw.usda.gov/GG2/index.shtml) was identified to be tightly linked to QLr.cau-1AS. Selection based on gpw2246 for QLr.cau-1AS was as effective as the selection based on cssfr5 for Lr34. QLr.cau-1AS will be helpful for increasing the genetic diversity of slow leaf-rusting resistance in wheat breeding programs. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/25982130/A_QTL_with_major_effect_on_reducing_leaf_rust_severity_on_the_short_arm_of_chromosome_1A_of_wheat_detected_across_different_genetic_backgrounds_and_diverse_environments_ L2 - https://dx.doi.org/10.1007/s00122-015-2533-x DB - PRIME DP - Unbound Medicine ER -