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A simultaneous electrochemical multianalyte immunoassay of high sensitivity C-reactive protein and soluble CD40 ligand based on reduced graphene oxide-tetraethylene pentamine that directly adsorb metal ions as labels.
Biosens Bioelectron. 2015 Oct 15; 72:237-46.BB

Abstract

A simplified electrochemical multianalyte immunosensor for the simultaneous detection of high sensitivity C-reactive protein (hsCRP) and soluble CD40 ligand (sCD40L) that uses reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) that directly adsorbs metal ions as labels is reported. rGO-TEPA contains a large number of amino groups and has excellent conductivity, making it an ideal template for the loading of Pb(2+) and Cu(2+), which greatly amplifies the detection signals. The signals could be directly detected in a single run through differential pulse voltammetry (DPV), and each biorecognition event produces a distinct voltammetric peak. The position and size of each peak reflects the identity and the level of the corresponding antigen. Primarily designed for an application in a sandwich-type immunoassay based on Pb(2+) and Cu(2+) labels, two main challenges are accomplished with the herein presented nanosheets: fabrication of the template and the amination process for Pb(2+) and Cu(2+) adsorption. To further improve the analytical performance of the immunosensor, Au@bovine serum albumin (BSA) nanospheres synthesized through a "green" synthesis route were used as a sensor platform, which not only provides a biocompatible microenvironment for the immobilization of antibodies but also amplifies the electrochemical signals. Under optimal conditions, hsCRP and sCD40L could be assayed in the range of 0.05 to 100 ng mL(-1) with detection limits of 16.7 and 13.1 pg mL(-1) (S/N=3), respectively. The assay results on clinical serum samples with the proposed immunosensor were in acceptable agreement with those using the standard single-analyte test of the enzyme-linked immunosorbent assay (ELISA). This novel immunosensing system provides a simple, sensitive and low-cost approach for a multianalyte immunoassay.

Authors+Show Affiliations

Institute of Life Science and School of Public Health, Chongqing Medical University, Chongqing 400016, PR China.Institute of Life Science and School of Public Health, Chongqing Medical University, Chongqing 400016, PR China.Institute of Life Science and School of Public Health, Chongqing Medical University, Chongqing 400016, PR China.Institute of Life Science and School of Public Health, Chongqing Medical University, Chongqing 400016, PR China.Institute of Life Science and School of Public Health, Chongqing Medical University, Chongqing 400016, PR China.Institute of Life Science and School of Public Health, Chongqing Medical University, Chongqing 400016, PR China.Institute of Life Science and School of Public Health, Chongqing Medical University, Chongqing 400016, PR China. Electronic address: hejunlin_11@aliyun.com.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25985199

Citation

Yuan, Guolin, et al. "A Simultaneous Electrochemical Multianalyte Immunoassay of High Sensitivity C-reactive Protein and Soluble CD40 Ligand Based On Reduced Graphene Oxide-tetraethylene Pentamine That Directly Adsorb Metal Ions as Labels." Biosensors & Bioelectronics, vol. 72, 2015, pp. 237-46.
Yuan G, Yu C, Xia C, et al. A simultaneous electrochemical multianalyte immunoassay of high sensitivity C-reactive protein and soluble CD40 ligand based on reduced graphene oxide-tetraethylene pentamine that directly adsorb metal ions as labels. Biosens Bioelectron. 2015;72:237-46.
Yuan, G., Yu, C., Xia, C., Gao, L., Xu, W., Li, W., & He, J. (2015). A simultaneous electrochemical multianalyte immunoassay of high sensitivity C-reactive protein and soluble CD40 ligand based on reduced graphene oxide-tetraethylene pentamine that directly adsorb metal ions as labels. Biosensors & Bioelectronics, 72, 237-46. https://doi.org/10.1016/j.bios.2015.04.088
Yuan G, et al. A Simultaneous Electrochemical Multianalyte Immunoassay of High Sensitivity C-reactive Protein and Soluble CD40 Ligand Based On Reduced Graphene Oxide-tetraethylene Pentamine That Directly Adsorb Metal Ions as Labels. Biosens Bioelectron. 2015 Oct 15;72:237-46. PubMed PMID: 25985199.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A simultaneous electrochemical multianalyte immunoassay of high sensitivity C-reactive protein and soluble CD40 ligand based on reduced graphene oxide-tetraethylene pentamine that directly adsorb metal ions as labels. AU - Yuan,Guolin, AU - Yu,Chao, AU - Xia,Chunyong, AU - Gao,Liuliu, AU - Xu,Wailan, AU - Li,Wenjuan, AU - He,Junlin, Y1 - 2015/04/30/ PY - 2015/02/28/received PY - 2015/04/09/revised PY - 2015/04/27/accepted PY - 2015/5/19/entrez PY - 2015/5/20/pubmed PY - 2016/3/2/medline KW - High sensitive C-reactive protein KW - Metal ion labels KW - Reduced graphene oxide-tetraethylene pentamine KW - Simultaneous detection KW - Soluble CD40 ligand SP - 237 EP - 46 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 72 N2 - A simplified electrochemical multianalyte immunosensor for the simultaneous detection of high sensitivity C-reactive protein (hsCRP) and soluble CD40 ligand (sCD40L) that uses reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) that directly adsorbs metal ions as labels is reported. rGO-TEPA contains a large number of amino groups and has excellent conductivity, making it an ideal template for the loading of Pb(2+) and Cu(2+), which greatly amplifies the detection signals. The signals could be directly detected in a single run through differential pulse voltammetry (DPV), and each biorecognition event produces a distinct voltammetric peak. The position and size of each peak reflects the identity and the level of the corresponding antigen. Primarily designed for an application in a sandwich-type immunoassay based on Pb(2+) and Cu(2+) labels, two main challenges are accomplished with the herein presented nanosheets: fabrication of the template and the amination process for Pb(2+) and Cu(2+) adsorption. To further improve the analytical performance of the immunosensor, Au@bovine serum albumin (BSA) nanospheres synthesized through a "green" synthesis route were used as a sensor platform, which not only provides a biocompatible microenvironment for the immobilization of antibodies but also amplifies the electrochemical signals. Under optimal conditions, hsCRP and sCD40L could be assayed in the range of 0.05 to 100 ng mL(-1) with detection limits of 16.7 and 13.1 pg mL(-1) (S/N=3), respectively. The assay results on clinical serum samples with the proposed immunosensor were in acceptable agreement with those using the standard single-analyte test of the enzyme-linked immunosorbent assay (ELISA). This novel immunosensing system provides a simple, sensitive and low-cost approach for a multianalyte immunoassay. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/25985199/A_simultaneous_electrochemical_multianalyte_immunoassay_of_high_sensitivity_C_reactive_protein_and_soluble_CD40_ligand_based_on_reduced_graphene_oxide_tetraethylene_pentamine_that_directly_adsorb_metal_ions_as_labels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(15)30091-9 DB - PRIME DP - Unbound Medicine ER -