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Measurement of carboxyhaemoglobin by spectrophotometry and gas chromatography.
Clin Physiol. 1989 Dec; 9(6):581-6.CP

Abstract

The purpose of this study was to evaluate state-of-the-art spectrophotometry for measurement of carboxyhaemoglobin (COHb). We measured the fractional concentration of COHb in 109 blood samples from patients under investigation of anaemia or with exposure to carbon monoxide (smokers) with the OSM3 Hemoximeter and by gas chromatography. Duplicate measurements were made with both methods in 42 samples. We found only a trivial systematic difference between the two methods. There was, however, a considerable scatter of the measurements, the limits of agreement (95% confidence limits for the difference between the two methods) being -0.98 and 0.86% COHb. The poor agreement between the methods was largely explained by a large random scatter in duplicate spectrophotometric measurements, whereas the method based on gas chromatography was highly reproducible. We conclude that the low accuracy of spectrophotometric measurements of COHb precludes its use for assessment of the endogenous production of CO, but that it may be useful for assessment of exposure to exogenous CO.

Authors+Show Affiliations

Johansson MB
Department of Clinical Physiology, University of Lund, Sweden.
Wollmer P
No affiliation info available

MeSH

AnemiaCarbon MonoxideCarboxyhemoglobinChromatography, GasHumansReference StandardsReproducibility of ResultsSmokingSpectrophotometry

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2598616

Citation

Johansson, M B., and P Wollmer. "Measurement of Carboxyhaemoglobin By Spectrophotometry and Gas Chromatography." Clinical Physiology (Oxford, England), vol. 9, no. 6, 1989, pp. 581-6.
Johansson MB, Wollmer P. Measurement of carboxyhaemoglobin by spectrophotometry and gas chromatography. Clin Physiol. 1989;9(6):581-6.
Johansson, M. B., & Wollmer, P. (1989). Measurement of carboxyhaemoglobin by spectrophotometry and gas chromatography. Clinical Physiology (Oxford, England), 9(6), 581-6.
Johansson MB, Wollmer P. Measurement of Carboxyhaemoglobin By Spectrophotometry and Gas Chromatography. Clin Physiol. 1989;9(6):581-6. PubMed PMID: 2598616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Measurement of carboxyhaemoglobin by spectrophotometry and gas chromatography. AU - Johansson,M B, AU - Wollmer,P, PY - 1989/12/1/pubmed PY - 1989/12/1/medline PY - 1989/12/1/entrez SP - 581 EP - 6 JF - Clinical physiology (Oxford, England) JO - Clin Physiol VL - 9 IS - 6 N2 - The purpose of this study was to evaluate state-of-the-art spectrophotometry for measurement of carboxyhaemoglobin (COHb). We measured the fractional concentration of COHb in 109 blood samples from patients under investigation of anaemia or with exposure to carbon monoxide (smokers) with the OSM3 Hemoximeter and by gas chromatography. Duplicate measurements were made with both methods in 42 samples. We found only a trivial systematic difference between the two methods. There was, however, a considerable scatter of the measurements, the limits of agreement (95% confidence limits for the difference between the two methods) being -0.98 and 0.86% COHb. The poor agreement between the methods was largely explained by a large random scatter in duplicate spectrophotometric measurements, whereas the method based on gas chromatography was highly reproducible. We conclude that the low accuracy of spectrophotometric measurements of COHb precludes its use for assessment of the endogenous production of CO, but that it may be useful for assessment of exposure to exogenous CO. SN - 0144-5979 UR - https://www.unboundmedicine.com/medline/citation/2598616/Measurement_of_carboxyhaemoglobin_by_spectrophotometry_and_gas_chromatography_ DB - PRIME DP - Unbound Medicine ER -