Tags

Type your tag names separated by a space and hit enter

The Autotransporter BpaB Contributes to the Virulence of Burkholderia mallei in an Aerosol Model of Infection.
PLoS One. 2015; 10(5):e0126437.Plos

Abstract

Burkholderia mallei is a highly pathogenic bacterium that causes the zoonosis glanders. Previous studies indicated that the genome of the organism contains eight genes specifying autotransporter proteins, which are important virulence factors of Gram-negative bacteria. In the present study, we report the characterization of one of these autotransporters, BpaB. Database searches identified the bpaB gene in ten B. mallei isolates and the predicted proteins were 99-100% identical. Comparative sequence analyses indicate that the gene product is a trimeric autotransporter of 1,090 amino acids with a predicted molecular weight of 105-kDa. Consistent with this finding, we discovered that recombinant bacteria expressing bpaB produce a protein of ≥ 300-kDa on their surface that is reactive with a BpaB-specific monoclonal antibody. Analysis of sera from mice infected with B. mallei indicated that animals produce antibodies against BpaB during the course of disease, thus establishing production of the autotransporter in vivo. To gain insight on its role in virulence, we inactivated the bpaB gene of B. mallei strain ATCC 23344 and determined the median lethal dose of the mutant in a mouse model of aerosol infection. These experiments revealed that the bpaB mutation attenuates virulence 8-14 fold. Using a crystal violet-based assay, we also discovered that constitutive production of BpaB on the surface of B. mallei promotes biofilm formation. To our knowledge, this is the first report of a biofilm factor for this organism.

Authors+Show Affiliations

Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, Georgia, United States of America.Department of Veterinary Biosciences and Diagnostic Imaging, University of Georgia College of Veterinary Medicine, Athens, GA, United States of America.Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, Georgia, United States of America; Department of Veterinary Biosciences and Diagnostic Imaging, University of Georgia College of Veterinary Medicine, Athens, GA, United States of America.Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, Georgia, United States of America.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

25993100

Citation

Zimmerman, Shawn M., et al. "The Autotransporter BpaB Contributes to the Virulence of Burkholderia Mallei in an Aerosol Model of Infection." PloS One, vol. 10, no. 5, 2015, pp. e0126437.
Zimmerman SM, Michel F, Hogan RJ, et al. The Autotransporter BpaB Contributes to the Virulence of Burkholderia mallei in an Aerosol Model of Infection. PLoS One. 2015;10(5):e0126437.
Zimmerman, S. M., Michel, F., Hogan, R. J., & Lafontaine, E. R. (2015). The Autotransporter BpaB Contributes to the Virulence of Burkholderia mallei in an Aerosol Model of Infection. PloS One, 10(5), e0126437. https://doi.org/10.1371/journal.pone.0126437
Zimmerman SM, et al. The Autotransporter BpaB Contributes to the Virulence of Burkholderia Mallei in an Aerosol Model of Infection. PLoS One. 2015;10(5):e0126437. PubMed PMID: 25993100.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Autotransporter BpaB Contributes to the Virulence of Burkholderia mallei in an Aerosol Model of Infection. AU - Zimmerman,Shawn M, AU - Michel,Frank, AU - Hogan,Robert J, AU - Lafontaine,Eric R, Y1 - 2015/05/20/ PY - 2015/02/03/received PY - 2015/04/02/accepted PY - 2015/5/21/entrez PY - 2015/5/21/pubmed PY - 2016/4/16/medline SP - e0126437 EP - e0126437 JF - PloS one JO - PLoS One VL - 10 IS - 5 N2 - Burkholderia mallei is a highly pathogenic bacterium that causes the zoonosis glanders. Previous studies indicated that the genome of the organism contains eight genes specifying autotransporter proteins, which are important virulence factors of Gram-negative bacteria. In the present study, we report the characterization of one of these autotransporters, BpaB. Database searches identified the bpaB gene in ten B. mallei isolates and the predicted proteins were 99-100% identical. Comparative sequence analyses indicate that the gene product is a trimeric autotransporter of 1,090 amino acids with a predicted molecular weight of 105-kDa. Consistent with this finding, we discovered that recombinant bacteria expressing bpaB produce a protein of ≥ 300-kDa on their surface that is reactive with a BpaB-specific monoclonal antibody. Analysis of sera from mice infected with B. mallei indicated that animals produce antibodies against BpaB during the course of disease, thus establishing production of the autotransporter in vivo. To gain insight on its role in virulence, we inactivated the bpaB gene of B. mallei strain ATCC 23344 and determined the median lethal dose of the mutant in a mouse model of aerosol infection. These experiments revealed that the bpaB mutation attenuates virulence 8-14 fold. Using a crystal violet-based assay, we also discovered that constitutive production of BpaB on the surface of B. mallei promotes biofilm formation. To our knowledge, this is the first report of a biofilm factor for this organism. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25993100/The_Autotransporter_BpaB_Contributes_to_the_Virulence_of_Burkholderia_mallei_in_an_Aerosol_Model_of_Infection_ L2 - https://dx.plos.org/10.1371/journal.pone.0126437 DB - PRIME DP - Unbound Medicine ER -