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Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes.
Sci Rep. 2015 May 21; 5:10552.SR

Abstract

Genomic in situ hybridization (GISH) has been widely used to detect rye (Secale cereale L.) chromosomes in wheat (Triticum aestivum L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of chromosomes and probes. In this study, new oligonucleotide probes Oligo-1162, Oligo-pSc200 and Oligo-pSc250 were developed. The three new probes can be used for non-denaturing fluorescence in situ hybridization (ND-FISH) assays and replace genomic DNA of rye as a probe to discriminate rye chromosomes in wheat backgrounds. In addition, previously developed oligonucleotide probes Oligo-pSc119.2-1, Oligo-pSc119.2-2, Oligo-pTa535-1, Oligo-pTa535-2, Oligo-pTa71-2, Oligo-pAWRC.1 and Oligo-CCS1 can also be used for ND-FISH of wheat and rye. These probes have provided an easier, faster and more cost-effective method for the FISH analysis of wheat and hybrids derived from wheat × rye.

Authors+Show Affiliations

1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, People's Republic of China.1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.1] Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China [2] Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25994088

Citation

Fu, Shulan, et al. "Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes." Scientific Reports, vol. 5, 2015, p. 10552.
Fu S, Chen L, Wang Y, et al. Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes. Sci Rep. 2015;5:10552.
Fu, S., Chen, L., Wang, Y., Li, M., Yang, Z., Qiu, L., Yan, B., Ren, Z., & Tang, Z. (2015). Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes. Scientific Reports, 5, 10552. https://doi.org/10.1038/srep10552
Fu S, et al. Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes. Sci Rep. 2015 May 21;5:10552. PubMed PMID: 25994088.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes. AU - Fu,Shulan, AU - Chen,Lei, AU - Wang,Yangyang, AU - Li,Meng, AU - Yang,Zujun, AU - Qiu,Ling, AU - Yan,Benju, AU - Ren,Zhenglong, AU - Tang,Zongxiang, Y1 - 2015/05/21/ PY - 2014/11/28/received PY - 2015/04/17/accepted PY - 2015/5/22/entrez PY - 2015/5/23/pubmed PY - 2016/3/29/medline SP - 10552 EP - 10552 JF - Scientific reports JO - Sci Rep VL - 5 N2 - Genomic in situ hybridization (GISH) has been widely used to detect rye (Secale cereale L.) chromosomes in wheat (Triticum aestivum L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of chromosomes and probes. In this study, new oligonucleotide probes Oligo-1162, Oligo-pSc200 and Oligo-pSc250 were developed. The three new probes can be used for non-denaturing fluorescence in situ hybridization (ND-FISH) assays and replace genomic DNA of rye as a probe to discriminate rye chromosomes in wheat backgrounds. In addition, previously developed oligonucleotide probes Oligo-pSc119.2-1, Oligo-pSc119.2-2, Oligo-pTa535-1, Oligo-pTa535-2, Oligo-pTa71-2, Oligo-pAWRC.1 and Oligo-CCS1 can also be used for ND-FISH of wheat and rye. These probes have provided an easier, faster and more cost-effective method for the FISH analysis of wheat and hybrids derived from wheat × rye. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/25994088/Oligonucleotide_Probes_for_ND_FISH_Analysis_to_Identify_Rye_and_Wheat_Chromosomes_ L2 - https://doi.org/10.1038/srep10552 DB - PRIME DP - Unbound Medicine ER -