TY - JOUR T1 - Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes. AU - Konishi,Jin, AU - Fukuda,Akira, AU - Mutaguchi,Kozue, AU - Uemura,Takeshi, Y1 - 2015/05/21/ PY - 2015/03/10/received PY - 2015/04/11/accepted PY - 2015/5/22/entrez PY - 2015/5/23/pubmed PY - 2016/3/22/medline SP - 1623 EP - 30 JF - Biotechnology letters JO - Biotechnol Lett VL - 37 IS - 8 N2 - OBJECTIVES: To genetically engineer Saccharomyces cerevisiae for improved ethanol productivity from glucose/xylose mixtures. RESULTS: An endogenous gene cassette composed of aldose reductase (GRE3), sorbitol dehydrogenase (SOR1) and xylulose kinase (XKS1) with a PGK1 promoter and a terminator was introduced into two S. cerevisiae strains, a laboratory strain (CEN.PK2-1C) and an industrial strain (Kyokai No. 7). The engineered Kyokai No. 7 strain (K7-XYL) exhibited a higher sugar consumption rate (1.03 g l(-1) h(-1)) and ethanol yield (63.8 %) from a glucose and xylose mixture compared to the engineered CEN.PK2-1C strain. Furthermore, K7-XYL produced a larger amount of ethanol (39.6 g l(-1)) compared to K7-SsXYL (32 g l(-1)) with integrated xylose reductase and xylitol dehydrogenase from a xylose-assimilating yeast Scheffersomyces stipitis instead of GRE3 and SOR1. CONCLUSION: The created S. cerevisiae strain showed sufficient xylose-fermenting ability to be used for efficient ethanol production from glucose/xylose. SN - 1573-6776 UR - https://www.unboundmedicine.com/medline/citation/25994575/Xylose_fermentation_by_Saccharomyces_cerevisiae_using_endogenous_xylose_assimilating_genes_ DB - PRIME DP - Unbound Medicine ER -