Tags

Type your tag names separated by a space and hit enter

Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes.
Biotechnol Lett. 2015 Aug; 37(8):1623-30.BL

Abstract

OBJECTIVES

To genetically engineer Saccharomyces cerevisiae for improved ethanol productivity from glucose/xylose mixtures.

RESULTS

An endogenous gene cassette composed of aldose reductase (GRE3), sorbitol dehydrogenase (SOR1) and xylulose kinase (XKS1) with a PGK1 promoter and a terminator was introduced into two S. cerevisiae strains, a laboratory strain (CEN.PK2-1C) and an industrial strain (Kyokai No. 7). The engineered Kyokai No. 7 strain (K7-XYL) exhibited a higher sugar consumption rate (1.03 g l(-1) h(-1)) and ethanol yield (63.8 %) from a glucose and xylose mixture compared to the engineered CEN.PK2-1C strain. Furthermore, K7-XYL produced a larger amount of ethanol (39.6 g l(-1)) compared to K7-SsXYL (32 g l(-1)) with integrated xylose reductase and xylitol dehydrogenase from a xylose-assimilating yeast Scheffersomyces stipitis instead of GRE3 and SOR1.

CONCLUSION

The created S. cerevisiae strain showed sufficient xylose-fermenting ability to be used for efficient ethanol production from glucose/xylose.

Authors+Show Affiliations

Bio Fuels & Chemicals R&D Group, Frontier Research Laboratory, Central Technical Research Laboratory, JX Nippon Oil & Energy Corporation, 8, Chidori-cho, Naka-Ku, Yokohama, 231-0815, Japan, jin.konishi@noe.jx-group.co.jp.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25994575

Citation

Konishi, Jin, et al. "Xylose Fermentation By Saccharomyces Cerevisiae Using Endogenous Xylose-assimilating Genes." Biotechnology Letters, vol. 37, no. 8, 2015, pp. 1623-30.
Konishi J, Fukuda A, Mutaguchi K, et al. Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes. Biotechnol Lett. 2015;37(8):1623-30.
Konishi, J., Fukuda, A., Mutaguchi, K., & Uemura, T. (2015). Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes. Biotechnology Letters, 37(8), 1623-30. https://doi.org/10.1007/s10529-015-1840-2
Konishi J, et al. Xylose Fermentation By Saccharomyces Cerevisiae Using Endogenous Xylose-assimilating Genes. Biotechnol Lett. 2015;37(8):1623-30. PubMed PMID: 25994575.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes. AU - Konishi,Jin, AU - Fukuda,Akira, AU - Mutaguchi,Kozue, AU - Uemura,Takeshi, Y1 - 2015/05/21/ PY - 2015/03/10/received PY - 2015/04/11/accepted PY - 2015/5/22/entrez PY - 2015/5/23/pubmed PY - 2016/3/22/medline SP - 1623 EP - 30 JF - Biotechnology letters JO - Biotechnol Lett VL - 37 IS - 8 N2 - OBJECTIVES: To genetically engineer Saccharomyces cerevisiae for improved ethanol productivity from glucose/xylose mixtures. RESULTS: An endogenous gene cassette composed of aldose reductase (GRE3), sorbitol dehydrogenase (SOR1) and xylulose kinase (XKS1) with a PGK1 promoter and a terminator was introduced into two S. cerevisiae strains, a laboratory strain (CEN.PK2-1C) and an industrial strain (Kyokai No. 7). The engineered Kyokai No. 7 strain (K7-XYL) exhibited a higher sugar consumption rate (1.03 g l(-1) h(-1)) and ethanol yield (63.8 %) from a glucose and xylose mixture compared to the engineered CEN.PK2-1C strain. Furthermore, K7-XYL produced a larger amount of ethanol (39.6 g l(-1)) compared to K7-SsXYL (32 g l(-1)) with integrated xylose reductase and xylitol dehydrogenase from a xylose-assimilating yeast Scheffersomyces stipitis instead of GRE3 and SOR1. CONCLUSION: The created S. cerevisiae strain showed sufficient xylose-fermenting ability to be used for efficient ethanol production from glucose/xylose. SN - 1573-6776 UR - https://www.unboundmedicine.com/medline/citation/25994575/Xylose_fermentation_by_Saccharomyces_cerevisiae_using_endogenous_xylose_assimilating_genes_ L2 - https://doi.org/10.1007/s10529-015-1840-2 DB - PRIME DP - Unbound Medicine ER -