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Usage of Leptospira spp. local strains as antigens increases the sensitivity of the serodiagnosis of bovine leptospirosis.
Acta Trop 2015; 149:163-7AT

Abstract

Leptospirosis is a zoonotic disease that occurs worldwide, particularly in tropical countries. In livestock the agent is responsible for reproductive problems such as infertility and abortion. Serogroup Sejroe, particularly serovar Hardjo, prevails in cattle in several regions. The microscopic agglutination test (MAT) is the current method for diagnosing leptospirosis. It has been proposed that the inclusion of local strains could detect a larger set of seroreactive animals. In that context, the aim of the present study was to evaluate if the usage of local strains as antigens increases the sensitivity of the serodiagnosis of bovine leptospirosis. Blood and urine samples were collected from 314 bovines from several herds randomly selected in a slaughterhouse in Rio de Janeiro, Brazil. Serological diagnosis was made with MAT using a 21 reference-strains panel (MAT21). Additionally, 12 local strains (MAT33) were included as antigens. PCR was performed with the urine samples and it was positive on 71 out of 222 samples (31.9%). MAT21 identified as seroreactive 173 (55.1%) out of the 314 animals studied, with Sejroe the most common (38.1%). In MAT33, 204 (65.0%) animals were seroreactive with a significant increase on seroreactivity (9.9%). In conclusion, MAT presented with a significant increase of sensitivity when local strains were used as antigens. Among the local strains, 2013_U152 (KP263062) (serogroup Shermani) and 2013_U280 (KP263069) (serogroup Grippotyphosa) showed to be more antigenic.

Authors+Show Affiliations

Fluminense Federal University, Laboratory of Veterinary Bacteriology, Biomedical Institute, 101 Prof. Hernani Mello Street, Niterói, Rio de Janeiro, Brazil.Fluminense Federal University, Laboratory of Veterinary Bacteriology, Biomedical Institute, 101 Prof. Hernani Mello Street, Niterói, Rio de Janeiro, Brazil.Fluminense Federal University, Laboratory of Veterinary Bacteriology, Biomedical Institute, 101 Prof. Hernani Mello Street, Niterói, Rio de Janeiro, Brazil.Fluminense Federal University, Laboratory of Veterinary Bacteriology, Biomedical Institute, 101 Prof. Hernani Mello Street, Niterói, Rio de Janeiro, Brazil. Electronic address: mipwalt@vm.uff.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25997883

Citation

Pinto, Priscila S., et al. "Usage of Leptospira Spp. Local Strains as Antigens Increases the Sensitivity of the Serodiagnosis of Bovine Leptospirosis." Acta Tropica, vol. 149, 2015, pp. 163-7.
Pinto PS, Loureiro AP, Penna B, et al. Usage of Leptospira spp. local strains as antigens increases the sensitivity of the serodiagnosis of bovine leptospirosis. Acta Trop. 2015;149:163-7.
Pinto, P. S., Loureiro, A. P., Penna, B., & Lilenbaum, W. (2015). Usage of Leptospira spp. local strains as antigens increases the sensitivity of the serodiagnosis of bovine leptospirosis. Acta Tropica, 149, pp. 163-7. doi:10.1016/j.actatropica.2015.05.008.
Pinto PS, et al. Usage of Leptospira Spp. Local Strains as Antigens Increases the Sensitivity of the Serodiagnosis of Bovine Leptospirosis. Acta Trop. 2015;149:163-7. PubMed PMID: 25997883.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Usage of Leptospira spp. local strains as antigens increases the sensitivity of the serodiagnosis of bovine leptospirosis. AU - Pinto,Priscila S, AU - Loureiro,Ana P, AU - Penna,Bruno, AU - Lilenbaum,Walter, Y1 - 2015/05/19/ PY - 2014/10/31/received PY - 2015/04/29/revised PY - 2015/05/12/accepted PY - 2015/5/23/entrez PY - 2015/5/23/pubmed PY - 2016/3/10/medline KW - Leptospirosis KW - Local strains KW - MAT SP - 163 EP - 7 JF - Acta tropica JO - Acta Trop. VL - 149 N2 - Leptospirosis is a zoonotic disease that occurs worldwide, particularly in tropical countries. In livestock the agent is responsible for reproductive problems such as infertility and abortion. Serogroup Sejroe, particularly serovar Hardjo, prevails in cattle in several regions. The microscopic agglutination test (MAT) is the current method for diagnosing leptospirosis. It has been proposed that the inclusion of local strains could detect a larger set of seroreactive animals. In that context, the aim of the present study was to evaluate if the usage of local strains as antigens increases the sensitivity of the serodiagnosis of bovine leptospirosis. Blood and urine samples were collected from 314 bovines from several herds randomly selected in a slaughterhouse in Rio de Janeiro, Brazil. Serological diagnosis was made with MAT using a 21 reference-strains panel (MAT21). Additionally, 12 local strains (MAT33) were included as antigens. PCR was performed with the urine samples and it was positive on 71 out of 222 samples (31.9%). MAT21 identified as seroreactive 173 (55.1%) out of the 314 animals studied, with Sejroe the most common (38.1%). In MAT33, 204 (65.0%) animals were seroreactive with a significant increase on seroreactivity (9.9%). In conclusion, MAT presented with a significant increase of sensitivity when local strains were used as antigens. Among the local strains, 2013_U152 (KP263062) (serogroup Shermani) and 2013_U280 (KP263069) (serogroup Grippotyphosa) showed to be more antigenic. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/25997883/Usage_of_Leptospira_spp__local_strains_as_antigens_increases_the_sensitivity_of_the_serodiagnosis_of_bovine_leptospirosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(15)30001-2 DB - PRIME DP - Unbound Medicine ER -