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mTOR is necessary for proper satellite cell activity and skeletal muscle regeneration.
Biochem Biophys Res Commun. 2015 Jul 17-24; 463(1-2):102-8.BB

Abstract

The serine/threonine kinase mammalian target of rapamycin (mTOR) is a key regulator of protein synthesis, cell proliferation and energy metabolism. As constitutive deletion of Mtor gene results in embryonic lethality, the function of mTOR in muscle stem cells (satellite cells) and skeletal muscle regeneration remains to be determined. In this study, we established a satellite cell specific Mtor conditional knockout (cKO) mouse model by crossing Pax7(CreER) and Mtor(flox/flox) mice. Skeletal muscle regeneration after injury was severely compromised in the absence of Mtor, indicated by increased number of necrotic myofibers infiltrated by Evans blue dye, and reduced number and size of regenerated myofibers in the Mtor cKO mice compared to wild type (WT) littermates. To dissect the cellular mechanism, we analyzed satellite cell-derived primary myoblasts grown on single myofibers or adhered to culture plates. The Mtor cKO myoblasts exhibited defective proliferation and differentiation kinetics when compared to myoblasts derived from WT littermates. At the mRNA and protein levels, the Mtor cKO myoblasts expressed lower levels of key myogenic determinant genes Pax7, Myf5, Myod, Myog than did the WT myoblasts. These results suggest that mTOR is essential for satellite cell function and skeletal muscle regeneration through controlling the expression of myogenic genes.

Authors+Show Affiliations

Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: zhengrong@mail.hzau.edu.cn.Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA. Electronic address: skuang@purdue.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25998386

Citation

Zhang, Pengpeng, et al. "MTOR Is Necessary for Proper Satellite Cell Activity and Skeletal Muscle Regeneration." Biochemical and Biophysical Research Communications, vol. 463, no. 1-2, 2015, pp. 102-8.
Zhang P, Liang X, Shan T, et al. MTOR is necessary for proper satellite cell activity and skeletal muscle regeneration. Biochem Biophys Res Commun. 2015;463(1-2):102-8.
Zhang, P., Liang, X., Shan, T., Jiang, Q., Deng, C., Zheng, R., & Kuang, S. (2015). MTOR is necessary for proper satellite cell activity and skeletal muscle regeneration. Biochemical and Biophysical Research Communications, 463(1-2), 102-8. https://doi.org/10.1016/j.bbrc.2015.05.032
Zhang P, et al. MTOR Is Necessary for Proper Satellite Cell Activity and Skeletal Muscle Regeneration. Biochem Biophys Res Commun. 2015 Jul 17-24;463(1-2):102-8. PubMed PMID: 25998386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - mTOR is necessary for proper satellite cell activity and skeletal muscle regeneration. AU - Zhang,Pengpeng, AU - Liang,Xinrong, AU - Shan,Tizhong, AU - Jiang,Qinyang, AU - Deng,Changyan, AU - Zheng,Rong, AU - Kuang,Shihuan, Y1 - 2015/05/18/ PY - 2015/05/05/received PY - 2015/05/09/accepted PY - 2015/5/23/entrez PY - 2015/5/23/pubmed PY - 2015/8/25/medline KW - Muscle regeneration KW - Satellite cells KW - Skeletal muscle KW - mTOR SP - 102 EP - 8 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 463 IS - 1-2 N2 - The serine/threonine kinase mammalian target of rapamycin (mTOR) is a key regulator of protein synthesis, cell proliferation and energy metabolism. As constitutive deletion of Mtor gene results in embryonic lethality, the function of mTOR in muscle stem cells (satellite cells) and skeletal muscle regeneration remains to be determined. In this study, we established a satellite cell specific Mtor conditional knockout (cKO) mouse model by crossing Pax7(CreER) and Mtor(flox/flox) mice. Skeletal muscle regeneration after injury was severely compromised in the absence of Mtor, indicated by increased number of necrotic myofibers infiltrated by Evans blue dye, and reduced number and size of regenerated myofibers in the Mtor cKO mice compared to wild type (WT) littermates. To dissect the cellular mechanism, we analyzed satellite cell-derived primary myoblasts grown on single myofibers or adhered to culture plates. The Mtor cKO myoblasts exhibited defective proliferation and differentiation kinetics when compared to myoblasts derived from WT littermates. At the mRNA and protein levels, the Mtor cKO myoblasts expressed lower levels of key myogenic determinant genes Pax7, Myf5, Myod, Myog than did the WT myoblasts. These results suggest that mTOR is essential for satellite cell function and skeletal muscle regeneration through controlling the expression of myogenic genes. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/25998386/mTOR_is_necessary_for_proper_satellite_cell_activity_and_skeletal_muscle_regeneration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(15)00946-8 DB - PRIME DP - Unbound Medicine ER -