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The function of PlcR in Bacillus anthracis vaccine strain A16R.
Yi Chuan 2015; 37(5):494-8YC

Abstract

Bacillus anthracis, B. thuringiensis and B. cereus are members of the B. cereus group. They share high genetic similarity. Whereas plcR (Phospholipase C regulator) usually encodes a functional pleiotropic activator protein in B. cereus and B. thuringiensis isolates, a characteristic nonsense mutation is found in all B. anthracis strains investigated, making the gene dysfunctional. To study the function of PlcR in B. anthracis, we used the B. cereus CMCC63301 genome as a template and constructed a recombinant expression plasmid pBE2A-plcR, and introduced it into the B. anthracis vaccine strain A16R, and then analyzed the activity of the hemolysin and sphingomyelinase. The results showed that transformation of B. anthracis with plasmid pBE2A-plcR carrying the native B. cereus plcR gene active the expression of sphingomyelinase gene, but did not activate expression of hemolysin genes of B. anthracis A16R.

Authors+Show Affiliations

1. Ministry of Education Key Laboratory for Tropical Biological Resources, Hainan University, Haikou 570228,China; 2. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.1. Ministry of Education Key Laboratory for Tropical Biological Resources, Hainan University, Haikou 570228,China; 2. Hainan Key Laboratory for Sustainable Utilization of Tropical Bio-resources-State Key Laboratory Incubation Base, Haikou 570228, China;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.Ministry of Education Key Laboratory for Tropical Biological Resources, Hainan University, Haikou 570228,China.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25998439

Citation

Jia, Xiao-lin, et al. "The Function of PlcR in Bacillus Anthracis Vaccine Strain A16R." Yi Chuan = Hereditas, vol. 37, no. 5, 2015, pp. 494-8.
Jia XL, Wang DS, Gao ZQ, et al. The function of PlcR in Bacillus anthracis vaccine strain A16R. Yi Chuan. 2015;37(5):494-8.
Jia, X. L., Wang, D. S., Gao, Z. Q., Feng, E. L., Zheng, J. P., Wang, H. L., ... Liu, X. K. (2015). The function of PlcR in Bacillus anthracis vaccine strain A16R. Yi Chuan = Hereditas, 37(5), pp. 494-8. doi:10.16288/j.yczz.15-040.
Jia XL, et al. The Function of PlcR in Bacillus Anthracis Vaccine Strain A16R. Yi Chuan. 2015;37(5):494-8. PubMed PMID: 25998439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The function of PlcR in Bacillus anthracis vaccine strain A16R. AU - Jia,Xiao-lin, AU - Wang,Dong-shu, AU - Gao,Zhi-qi, AU - Feng,Er-ling, AU - Zheng,Ji-ping, AU - Wang,Heng-liang, AU - Guo,Gui-ying, AU - Liu,Xian-kai, PY - 2015/5/23/entrez PY - 2015/5/23/pubmed PY - 2015/7/3/medline SP - 494 EP - 8 JF - Yi chuan = Hereditas JO - Yi Chuan VL - 37 IS - 5 N2 - Bacillus anthracis, B. thuringiensis and B. cereus are members of the B. cereus group. They share high genetic similarity. Whereas plcR (Phospholipase C regulator) usually encodes a functional pleiotropic activator protein in B. cereus and B. thuringiensis isolates, a characteristic nonsense mutation is found in all B. anthracis strains investigated, making the gene dysfunctional. To study the function of PlcR in B. anthracis, we used the B. cereus CMCC63301 genome as a template and constructed a recombinant expression plasmid pBE2A-plcR, and introduced it into the B. anthracis vaccine strain A16R, and then analyzed the activity of the hemolysin and sphingomyelinase. The results showed that transformation of B. anthracis with plasmid pBE2A-plcR carrying the native B. cereus plcR gene active the expression of sphingomyelinase gene, but did not activate expression of hemolysin genes of B. anthracis A16R. SN - 0253-9772 UR - https://www.unboundmedicine.com/medline/citation/25998439/The_function_of_PlcR_in_Bacillus_anthracis_vaccine_strain_A16R_ DB - PRIME DP - Unbound Medicine ER -